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MagPiezo enables wireless activation of endogenous Piezo1 channels without genetic modification using 19 nm magnetic nanoparticles and low‐intensity magnetic fields. It generates torque forces at the piconewton scale to trigger mechanotransduction in endothelial cells, standing as a novel platform to interrogate and manipulate Piezo1 activity in vitro.
Susel Del Sol‐Fernández +7 more
wiley +1 more source
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The Metabolome Weakens RNA Thermodynamic Stability and Strengthens RNA Chemical Stability
Biochemistry, 2022We examined the complex network of interactions among RNA, the metabolome, and divalent Mg2+ under conditions that mimic the Escherichia coli cytoplasm. We determined Mg2+ binding constants for the top 15 E. coli metabolites, comprising 80% of the metabolome by concentration at physiological pH and monovalent ion concentrations. These data were used to
Jacob P. Sieg +4 more
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Control of RNA Stability in Immunity
Annual Review of Immunology, 2021Posttranscriptional control of mRNA regulates various biological processes, including inflammatory and immune responses. RNA-binding proteins (RBPs) bind cis-regulatory elements in the 3′ untranslated regions (UTRs) of mRNA and regulate mRNA turnover and translation.
Shizuo, Akira, Kazuhiko, Maeda
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Thermodynamic Studies of RNA Stability
Journal of Biomolecular Structure and Dynamics, 1984Enthalpies and entropies of helix stabilization due to addition of 3' terminal unpaired nucleotides to a CCGG or GGCC core double helix are derived from UV melting studies. The results suggest stacking provides a significant fraction of the free energy of a terminal base pair.
S M, Freier +3 more
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RNA stabilization in hyperthermophilic archaea
Annals of the New York Academy of Sciences, 2019AbstractAnalyses of the RNA metabolism of hyperthermophilic archaea highlight the efficiency of regulatory RNAs and RNA‐guided processes at extreme temperatures. These organisms must overcome the intrinsic thermolability of RNAs. Elevated levels of RNA modifications and structured GC‐rich regions are observed for many universal noncoding RNA families ...
Gomes-Filho, J., Randau, L.
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Chloroplast RNA processing and stability
Photosynthesis Research, 2004Primary chloroplast transcripts are processed in a number of ways, including intron splicing, internal cleavage of polycistronic RNAs, and endonucleolytic or exonucleolytic cleavages at the transcript termini. All chloroplast RNAs are also subject to degradation, although a curious feature of many chloroplast mRNAs is their relative longevity.
David L, Herrin, Jöerg, Nickelsen
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Regulation of RNA Stability Through RNA Modification
2021As intracellular RNA levels are determined by the balance between RNA synthesis and decay, RNA stability is a critical factor in the quantity of intracellular RNA. Recent advances in epitranscriptomic measuring techniques have shown that RNA modifications, such as methylation and acetylation, affect RNA stability, including those of messenger RNA (mRNA)
Kentaro Kawata, Nobuyoshi Akimitsu
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Seminars in Virology, 1997
Abstract RNA molecules fold into specific base-paired conformations that contain single-stranded regions, A-form double helices, hairpin loops, internal loops, bulges, junctions, pseudoknots, kissing hairpins, and so forth. These structural motifs are recognized by proteins, other RNAs, and other parts of the same RNA.
Jacek Nowakowski, Ignacio Tinoco
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Abstract RNA molecules fold into specific base-paired conformations that contain single-stranded regions, A-form double helices, hairpin loops, internal loops, bulges, junctions, pseudoknots, kissing hairpins, and so forth. These structural motifs are recognized by proteins, other RNAs, and other parts of the same RNA.
Jacek Nowakowski, Ignacio Tinoco
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Gene expression regulated by RNA stability
Science, 2020The factor responsible for autoregulation of tubulin RNA stability is ...
Ofer, Shoshani, Don W, Cleveland
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