Results 31 to 40 of about 1,193,020 (266)

ArfB can displace mRNA to rescue stalled ribosomes

Nature Communications, 2020
Alternative rescue factor B (ArfB) is an enzyme that releases peptides from stalled ribosomes to allow ribosome recycling. Here the authors carry-out cryo-EM analyses of 70S ribosomes complexed with ArfB on either a short or longer mRNA to reveal ...
Christine E. Carbone, Gabriel Demo, Rohini Madireddy, Egor Svidritskiy, Andrei A. Korostelev   +4 more
doaj   +1 more source

HDAC1 SUMOylation promotes Argonaute-directed transcriptional silencing in C. elegans

eLife, 2021
Eukaryotic cells use guided search to coordinately control dispersed genetic elements. Argonaute proteins and their small RNA cofactors engage nascent RNAs and chromatin-associated proteins to direct transcriptional silencing.
Heesun Kim, Yue-He Ding, Gangming Zhang, Yong-Hong Yan, Darryl Conte Jr, Meng-Qiu Dong, Craig C Mello   +6 more
doaj   +1 more source

Nucleic Acid Therapeutics for Neurological Diseases

, 2020
Oligonucleotide therapeutics are emerging as a promising class of therapeutics in various areas of human health. In the long run, oligonucleotides are likely to become a major class of therapeutics, on par with small molecules and biologics.
Watts, Jonathan K, Khvorova, Anastasia, Brown, Robert H. Jr.   +2 more
core   +1 more source

Understanding and repurposing CRISPR-mediated alternative splicing

Genome Biology, 2018
Two new studies refine our understanding of CRISPR-associated exon skipping and redefine its utility in engineering alternative splicing.
Jordan L. Smith, Haiwei Mou, Wen Xue
doaj   +1 more source

Chemical engineering of therapeutic siRNAs for allele-specific gene silencing in Huntington’s disease models

Nature Communications, 2022
Chemically modified siRNAs distinguish between mutant and normal huntingtin based on a single nucleotide difference and lower mutant huntingtin specifically in patient derived cells and in a mouse model of Huntington’s disease.
Faith Conroy, Rachael Miller, Julia F. Alterman, Matthew R. Hassler, Dimas Echeverria, Bruno M. D. C. Godinho, Emily G. Knox, Ellen Sapp, Jaquelyn Sousa, Ken Yamada, Farah Mahmood, Adel Boudi, Kimberly Kegel-Gleason, Marian DiFiglia, Neil Aronin, Anastasia Khvorova, Edith L. Pfister   +16 more
doaj   +1 more source

Heavily and fully modified RNAs guide efficient SpyCas9-mediated genome editing

Nature Communications, 2018
Resistance of gRNA to ubiquitous ribonucleases is required for CRISPR-Cas9-based therapeutics. Here, the authors explore chemical modifications at all positions of the crRNA guide and tracrRNA cofactor, and identify modified versions that are more potent
Aamir Mir, Julia F. Alterman, Matthew R. Hassler, Alexandre J. Debacker, Edward Hudgens, Dimas Echeverria, Michael H. Brodsky, Anastasia Khvorova, Jonathan K. Watts, Erik J. Sontheimer   +9 more
doaj   +1 more source

PIE-1 SUMOylation promotes germline fates and piRNA-dependent silencing in C. elegans

eLife, 2021
Germlines shape and balance heredity, integrating and regulating information from both parental and foreign sources. Insights into how germlines handle information have come from the study of factors that specify or maintain the germline fate.
Heesun Kim, Yue-He Ding, Shan Lu, Mei-Qing Zuo, Wendy Tan, Darryl Conte Jr, Meng-Qiu Dong, Craig C Mello   +7 more
doaj   +1 more source

ARID5B regulates fatty acid metabolism and proliferation at the Pre-B cell stage during B cell development

Frontiers in Immunology, 2023
During B cell development in bone marrow, large precursor B cells (large Pre-B cells) proliferate rapidly, exit the cell cycle, and differentiate into non-proliferative (quiescent) small Pre-B cells.
Jaya Prakash Chalise, Ali Ehsani, Mengistu Lemecha, Yu-Wen Hung, Guoxiang Zhang, Garrett P. Larson, Keiichi Itakura   +6 more
doaj   +1 more source

Intradermal delivery of lipophilic siRNAs enables prolonged skin retention and sustained gene silencing in a porcine model

Nature Communications
Small interfering RNAs (siRNAs) offer significant therapeutic potential; however, extrahepatic applications, particularly to the skin, remain a challenge.
Hassan H. Fakih, Mohammad Zain UI Abideen, Mohamad Omar Rachid, Katherine Y. Gross, Thomas J. R. Ormsby, Vella M. Ross, Rosemary Gagnon, Christopher Dahlke, Raymond C. Furgal, Clemens Lochmann, Ashley Summers, Hanadi F. Sleiman, Sylvia M. Fürst, Shanté N. Jackson, Juliana O. Gordilho, Claire Bouix-Peter, Thibaud Portal, Qi Tang, John E. Harris, Anastasia Khvorova, Carine Blanchard, Julia F. Alterman   +21 more
doaj   +1 more source

Ovarian Sex Cord Stromal Tumors in Children and Adolescents—The European Standard Clinical Practice Recommendations

Pediatric Blood &Cancer, EarlyView.
ABSTRACT As part of the European Cooperative Study Group for Paediatric Rare Tumours initiative, we developed standard clinical practice guidelines for ovarian sex cord stromal tumors, based on comprehensive national and international cohort analyses, literature review, and a final expert consensus conference.
Dominik T. Schneider, Kris‐Ann Schultz, Nora Schnitzler, Michael Abele, Tal Ben‐Ami, Ewa Bien, Gianni Bisogno, Ines B. Brecht, Maja Cesen, Andrea Ferrari, Michaela Kuhlen, Malgorzata A. Krawczyk, Ricardo Lopez‐Almaraz, Yves Reguerre, Calogero Virgone, Daniel Orbach   +15 more
wiley   +1 more source