Results 11 to 20 of about 1,193,020 (266)

Formamide significantly enhances the efficiency of chemical adenylation of RNA sequencing ligation adaptors [PDF]

, 2023
Preadenylated single-stranded DNA ligation adaptors are essential reagents in many next generation RNA sequencing library preparation protocols. These oligonucleotides can be adenylated enzymatically or chemically.
Watts, Jonathan K, Hildebrand, Samuel R, Hedger, Adam K, Khvorova, Anastasia, Yamada, Ken   +4 more
core   +1 more source

Antisense RNA Design, Delivery, and Analysis [PDF]

, 2022
This open access volume gathers a variety of models, delivery systems, and approaches that can be used to assess RNA technology for exploiting antisense as a therapeutic intervention.

core   +2 more sources

Novel endoribonucleases as central players in various pathways of eukaryotic RNA metabolism [PDF]

, 2010
For a long time it has been assumed that the decay of RNA in eukaryotes is mainly carried out by exoribonucleases, which is in contrast to bacteria, where endoribonucleases are well documented to initiate RNA degradation.
Tomecki, Rafał, Dziembowski, Andrzej
core   +3 more sources

Gene editing with CRISPR-Cas12a guides possessing ribose-modified pseudoknot handles [PDF]

, 2021
CRISPR-Cas12a is a leading technology for development of model organisms, therapeutics, and diagnostics. These applications could benefit from chemical modifications that stabilize or tune enzyme properties.
Damha, Masad J, Abdullahu, Leonora, O'Reilly, Daniel, Jana, Sunit, Jensik, Philip J, Ageely, Eman A, Chilamkurthy, Ramadevi, Gagnon, Keith T   +7 more
core   +1 more source

The RNA phosphatase PIR-1 regulates endogenous small RNA pathways in C. elegans [preprint] [PDF]

, 2020
Eukaryotic cells regulate 5' triphosphorylated (ppp-) RNAs to promote cellular functions and prevent recognition by antiviral RNA sensors. For example, RNA capping enzymes possess triphosphatase domains that remove the γ phosphates of ppp-RNAs during RNA
Li, Lichao, Moresco, James J., Yates, John R. III, Mello, Craig C., Gu, Weifeng, Dai, Hui, Chaves, Daniel A., Conte, Darryl Jr., Eun Oh, Myung   +8 more
core   +1 more source

RNA Interference by Single- and Double-stranded siRNA With a DNA Extension Containing a 3′ Nuclease-resistant Mini-hairpin Structure [PDF]

, 2013
Selective gene silencing by RNA interference (RNAi) involves double-stranded small interfering RNA (ds siRNA) composed of single-stranded (ss) guide and passenger RNAs.
Jo Milner, Simon J Allison, Milner, J., Allison, Simon J., Milner, Jo   +4 more
core   +1 more source

An automated Bayesian pipeline for rapid analysis of single-molecule binding data

Nature Communications, 2019
Analysis of single-molecule binding assays still requires substantial manual user intervention. Here, the authors present a pipeline for rapid, automated analysis of co-localization single-molecule spectroscopy images, with a modular user interface that ...
Carlas S. Smith, Karina Jouravleva, Maximiliaan Huisman, Samson M. Jolly, Phillip D. Zamore, David Grunwald   +5 more
doaj   +1 more source

Trim25 is an RNA-specific activator of Lin28a/TuT4-mediated uridylation [PDF]

, 2014
RNA binding proteins have thousands of cellular RNA targets and often exhibit opposite or passive molecular functions. Lin28a is a conserved RNA binding protein involved in pluripotency and tumorigenesis that was previously shown to trigger TuT4-mediated
Nowak, Jakub S., Roy Choudhury, Nila, Rappsilber, Juri, Spoel, Steven H, Nila Roy Choudhury, Nowak, Jakub S, Zuo, Juan, Michlewski, Gracjan, Juan Zuo, Juri Rappsilber, Choudhury, Nila Roy, Steven H. Spoel, Gracjan Michlewski, Jakub S. Nowak, Spoel, Steven H.   +14 more
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Polymeric carriers for delivery of RNA cancer therapeutics [PDF]

, 2022
As research uncovers the underpinnings of cancer biology, new targeted therapies have been de-veloped. Many of these therapies are small molecules such as kinase inhibitors that target specific proteins, however only 1% of the genome encodes for proteins
Frampton, Adam E., Theoni K. Georgiou, Lythgoe, Mark P, Clark, J, Mark P. Lythgoe, Joana S. Correia, Ottaviani, S, Clark, James, Mirón-Barroso, Sofía, Krel, J, Alexandra Porter, Laura Tookman, Frampton, AE, Salvado Correia, J, Castellano, L, Lythgoe, M, Tookman, L, Georgiou, Theoni K., Frampton, Adam E, Theoni Georgiou, Porter, Alexandra E, James Clark, Tookman, Laura, Alexandra E. Porter, Mirón-Barroso, S, Castellano, Leandro, Georgiou, T, Correia, Joana S., Silvia Ottaviani, Krell, Jonathan, Sofía Mirón-Barroso, Correia, Joana S, Leandro Castellano, Georgiou, Theoni K, Joana Correia, Ottaviani, Silvia, Jonathan Krell, Adam Frampton, Mark Lythgoe, Porter, Alexandra E., Porter, A, Lythgoe, Mark P., Adam E. Frampton   +42 more
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Self-inactivating, all-in-one AAV vectors for precision Cas9 genome editing via homology-directed repair in vivo

Nature Communications, 2021
Long-term expression of Cas9 following precision genome editing in vivo may lead to undesirable consequences. Here we show that a single-vector, self-inactivating AAV system containing Cas9 nuclease, guide, and DNA donor can use homology-directed repair ...
Raed Ibraheim, Phillip W. L. Tai, Aamir Mir, Nida Javeed, Jiaming Wang, Tomás C. Rodríguez, Suk Namkung, Samantha Nelson, Eraj Shafiq Khokhar, Esther Mintzer, Stacy Maitland, Zexiang Chen, Yueying Cao, Emmanouela Tsagkaraki, Scot A. Wolfe, Dan Wang, Athma A. Pai, Wen Xue, Guangping Gao, Erik J. Sontheimer   +19 more
doaj   +1 more source