Formamide significantly enhances the efficiency of chemical adenylation of RNA sequencing ligation adaptors [PDF]
, 2023 Preadenylated single-stranded DNA ligation adaptors are essential reagents in many next generation RNA sequencing library preparation protocols. These oligonucleotides can be adenylated enzymatically or chemically.
Watts, Jonathan K +4 more
core +1 more source
A cytoplasmic complex mediates specific mRNA recognition and localization in yeast [PDF]
, 2011 In eukaryotes, hundreds of mRNAs are localized by specialized transport complexes. For localization, transcripts are recognized by RNA-binding proteins and incorporated into motor-containing messenger ribonucleoprotein particles (mRNPs).
Henning Urlaub +41 more
core +2 more sources
Sensitive extraction-free SARS-CoV-2 RNA virus detection using a chelating resin
iScience, 2021 Summary: Current conventional detection of SARS-CoV-2 involves collection of a patient’s sample with a nasopharyngeal swab, storage of the swab during transport in a viral transport medium, extraction of RNA, and quantitative reverse transcription PCR ...
Bin Guan +6 more
doaj +1 more source
A New Cell-to-Cell Transport Model for Potexviruses
Molecular Plant-Microbe Interactions, 2005 In the last five years, we have gained significant insight into the role of the Potexvirus proteins in virus movement and RNA silencing. Potexviruses require three movement proteins, named triple gene block (TGB)p1, TGBp2, andTGBp3, and the viral coat ...
Jeanmarie Verchot-Lubicz
doaj +1 more source
Detection of genome-scale ordered RNA structure (GORS) in genomes of positive-stranded RNA viruses:Implications for virus evolution and host persistence [PDF]
, 2004 Discrete RNA secondary and higher-order structures, typically local in extent, play a fundamental role in RNA virus replication. Using new bioinformatics analysis methods, we have identified genome-scale ordered RNA structure (GORS) in many genera and ...
Evans, David J +5 more
core +1 more source
, 2021 Robust methods are critical for testing the in vivo regulatory mechanism of RNA binding proteins. Here we report improvement of a protein–mRNA tethering assay to probe the function of an RNA binding protein in its natural context within the C.
Kimble, Judith +3 more
core +1 more source
Transport outlook 2009: Globalisation, crisis and transport [PDF]
, 2009 This second ITF Transport Outlook continues building towards a full-fledged Transport Outlook, building upon the first Outlook (JTRC, 20081). The 2008 Outlook investigated the relation between expected GDP evolution and the demand for road transport ...
International Transport Forum, OECD
core +1 more source
Domain orientation in the RNA helicase YxiN and the role of conformational changes for RNA unwinding [PDF]
, 2010 The RNA helicase YxiN from Bacillus subtilis is a member of the family of DEAD box proteins. YxiN is able to unwind RNA double strands in an ATP-dependent manner. The ability to catalyse RNA rearrangement is in vivo presumably necessary for the bacterial
Karow, Anne R.
core +1 more source
eLife, 2015 Microsatellite expansions are the leading cause of numerous neurodegenerative disorders. Here we demonstrate that GGGGCC and CAG microsatellite repeat RNAs associated with C9orf72 in amyotrophic lateral sclerosis/frontotemporal dementia and with ...
Alondra Schweizer Burguete +5 more
doaj +1 more source
RNA-dependent association with myosin IIA promotes F-actin-guided trafficking of the ELAV-like protein HuR to polysomes [PDF]
, 2013 The role of the mRNA-binding protein human antigen R (HuR) in stabilization and translation of AU-rich elements (ARE) containing mRNAs is well established.
Eberhardt, Wolfgang +3 more
core +1 more source