Results 361 to 370 of about 323,847 (388)
Some of the next articles are maybe not open access.
2014
Typically, genes are delivered to a cell to increase the expression of a protein. RNA interference (RNAi), however, is used to lower or eliminate the production of a protein. In a gene delivery sense, the technology is very similar to transfection or transduction.
openaire +2 more sources
Typically, genes are delivered to a cell to increase the expression of a protein. RNA interference (RNAi), however, is used to lower or eliminate the production of a protein. In a gene delivery sense, the technology is very similar to transfection or transduction.
openaire +2 more sources
Methods, 2011
RNA interference (RNAi)-mediated gene knockdown has developed into a routine method to assess gene function in cultured mammalian cells in a fast and easy manner. For the use of RNAi in mice, short hairpin (sh) RNAs expressed stably from the genome are a fast alternative to conventional knockout approaches.
Ralf Kühn +3 more
openaire +2 more sources
RNA interference (RNAi)-mediated gene knockdown has developed into a routine method to assess gene function in cultured mammalian cells in a fast and easy manner. For the use of RNAi in mice, short hairpin (sh) RNAs expressed stably from the genome are a fast alternative to conventional knockout approaches.
Ralf Kühn +3 more
openaire +2 more sources
RNAi-based therapeutics and tumor targeted delivery in cancer.
Advanced Drug Delivery Reviews, 2022Goknur Kara, G. Calin, B. Ozpolat
semanticscholar +1 more source
Current Opinion in Chemical Biology, 2001
In many species, double-stranded RNA can specifically and effectively silence genes. This newly discovered biological phenomenon, called RNA interference (RNAi), has practical implications for functional genomics. As shown by two recent reports, RNAi provides a rapid method to test the function of genes in the nematode Caenorhabditis elegans; most of ...
openaire +3 more sources
In many species, double-stranded RNA can specifically and effectively silence genes. This newly discovered biological phenomenon, called RNA interference (RNAi), has practical implications for functional genomics. As shown by two recent reports, RNAi provides a rapid method to test the function of genes in the nematode Caenorhabditis elegans; most of ...
openaire +3 more sources
Nature, 2004
The term RNAi — short for RNA interference — crops up again and again in biology research these days. This is in part because of its power as a laboratory tool, and in part because it is a widespread natural phenomenon.
Carl D. Novina, Phillip A. Sharp
openaire +2 more sources
The term RNAi — short for RNA interference — crops up again and again in biology research these days. This is in part because of its power as a laboratory tool, and in part because it is a widespread natural phenomenon.
Carl D. Novina, Phillip A. Sharp
openaire +2 more sources
Current Protocols in Molecular Biology, 2005
AbstractRNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus‐vectors.
Joanne Chory +2 more
openaire +3 more sources
AbstractRNA silencing in plants is a rapid and facile approach for assignment of gene function and virus resistance. It allows selective RNA degradation through a mechanism that is given specificity by short interfering RNAs. The RNA silencer sequences are normally delivered as transgenes or a part of virus‐vectors.
Joanne Chory +2 more
openaire +3 more sources
Trends in Biotechnology, 2004
Synthetic small interfering RNA (siRNA) has become a valuable tool for investigating gene function in cell culture. This success has led to high expectations for siRNA as a tool for in vivo investigation and as a platform for therapeutic development.
Zain Paroo, David R. Corey
openaire +3 more sources
Synthetic small interfering RNA (siRNA) has become a valuable tool for investigating gene function in cell culture. This success has led to high expectations for siRNA as a tool for in vivo investigation and as a platform for therapeutic development.
Zain Paroo, David R. Corey
openaire +3 more sources
2011
In the last decade, much progress has been made towards a basic understanding of RNA silencing mechanisms in plants, like in animals and other eukaryotes. Many events that were already known, such as pathogen-derived resistance, posttranscriptional gene silencing, and microRNA (miRNA)-mediated regulation, were found to share a fundamentally similar ...
openaire +2 more sources
In the last decade, much progress has been made towards a basic understanding of RNA silencing mechanisms in plants, like in animals and other eukaryotes. Many events that were already known, such as pathogen-derived resistance, posttranscriptional gene silencing, and microRNA (miRNA)-mediated regulation, were found to share a fundamentally similar ...
openaire +2 more sources
A genome-wide transgenic RNAi library for conditional gene inactivation in Drosophila
Nature, 2007G. Dietzl +13 more
semanticscholar +1 more source
Trends in Genetics, 2003
Just a couple of years ago, only biologists working with plants or Caenorhabditis elegans could use RNA-mediated interference (RNAi) technology to gain insight into gene function. However, the recent groundbreaking discovery that in vitro synthesized, 21- to 23-nucleotide, double-stranded RNAs can act as small interfering RNAs (siRNAs) to elicit gene ...
openaire +3 more sources
Just a couple of years ago, only biologists working with plants or Caenorhabditis elegans could use RNA-mediated interference (RNAi) technology to gain insight into gene function. However, the recent groundbreaking discovery that in vitro synthesized, 21- to 23-nucleotide, double-stranded RNAs can act as small interfering RNAs (siRNAs) to elicit gene ...
openaire +3 more sources