Results 101 to 110 of about 476,060 (306)
BMC Genomics, 2020 Background There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA ...
Jin Sung Jang +7 more
doaj +1 more source
Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks
Advanced Functional Materials, EarlyView.This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson +12 more
wiley +1 more source
Ramsey goodness of bounded degree trees [PDF]
arXiv, 2016 Given a pair of graphs $G$ and $H$, the Ramsey number $R(G,H)$ is the smallest $N$ such that every red-blue coloring of the edges of the complete graph $K_N$ contains a red copy of $G$ or a blue copy of $H$. If a graph $G$ is connected, it is well known and easy to show that $R(G,H) \geq (|G|-1)(\chi(H)-1)+\sigma(H)$, where $\chi(H)$ is the chromatic ...
arxiv
Lipid Nanoparticle‐Mediated CRISPR‐Cas13a Delivery for the Control of Bacterial Infection
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.New formulations of lipid nanoparticles (LNPs) that can deliver nucleic acids to Gram‐negative bacteria are proposed to combat bacterial infection. The delivery of nucleic acids by LNPs is aided by LNP‐helpers which weaken the bacterial outer membrane. LNPs encapsulating the Cas13a/gRNA expression vector achieve an antibacterial effect in both in vivo ...
Bookun Kim +9 more
wiley +1 more source
An inverse scattering problem for the Schrödinger equation in a semiclassical process [PDF]
arXiv, 2005 We study an inverse scattering problem for a pair of Hamiltonians $(H(h), H\_0 (h))$ on $L^2 (\r^n)$, where $H\_0 (h) = -h^2 \Delta$ and $H (h)= H\_0 (h) +V$, $V$ is a short-range potential with a regular behaviour at infinity and $h$ is the semiclassical parameter. We show that, in dimension $n \geq 3$, the knowledge of the scattering operators $S(h)$,
arxiv
Advanced Healthcare Materials, Volume 14, Issue 7, March 14, 2025.The top‐performing lipid nanoparticle, incorporating a novel ionizable lipid derived from vitamin B5, demonstrates high mRNA transfection efficiency, low toxicity, favorable stability, targeted delivery to lymphoid tissues, and high immunogenicity, showing promise as a carrier for mRNA vaccines for infectious diseases and cancer.
Soyeon Yoo +11 more
wiley +1 more source
On the irregularity of uniform hypergraphs [PDF]
arXiv, 2018 Let $H$ be an $r$-uniform hypergraph on $n$ vertices and $m$ edges, and let $d_i$ be the degree of $i\in V(H)$. Denote by $\varepsilon(H)$ the difference of the spectral radius of $H$ and the average degree of $H$. Also, denote \[ s(H)=\sum_{i\in V(H)}\left|d_i-\frac{rm}{n}\right|,~ v(H)=\frac{1}{n}\sum_{i\in V(H)}d_i^{\frac{r}{r-1}}-\left(\frac{rm}{n}\
arxiv
Advanced Healthcare Materials, EarlyView.Most in vitro cancer models lack cellular diversity, functional complexity, and clinical relevance. This work highlights the generation of an innovative dynamic tetraculture with autologous patient‐derived cells within a vascularized tumor‐on‐chip, as well as the recapitulation of endothelial anergy features induced by the tumor microenvironment ...
Christine Lansche +16 more
wiley +1 more source
Full-Length cDNA Synthesis for Long-Distance RT-PCR of Large mRNA Transcripts
BioTechniques, 2003 Reverse transcription of mRNA often leads to the synthesis of partial, non-fulllength cDNAs. Methods to facilitate reverse transcription across RNA regions of secondary structure, as well as enzyme modifications to eliminate RNase H activities inherent ...
Phillip R. Hawkins, Pei Jin, Glenn K. Fu
doaj +1 more source
Studies on reverse transcriptase of RNA tumor viruses. I. Localization of thermolabile DNA polymerase and RNase H activities on one polypeptide [PDF]
, 1975 Inder M. Verma
openalex +1 more source