Results 81 to 90 of about 303,152 (296)

Yeast RNase H(35) is the counterpart of the mammalian RNase HI, and is evolutionarily related to prokaryotic RNase HII1We dedicate this work to the memory of our colleague Robert Karwan (1959–1997).1

, 1998
We cloned the Saccharomyces cerevisiae homologue of mammalian RNase HI, which itself is related to the prokaryotic RNase HII, an enzyme of unknown function and previously described as having minor activity in Escherichia coli.
Braunshofer-Reiter, Christa, Peter Frank, Christa Braunshofer-Reiter, Frank, Peter, Wintersberger, Ulrike, Ulrike Wintersberger   +5 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

The Dual Role of Histidine as General Base and Recruiter of a Third Metal Ion in HIV-1 RNase H

, 2019
RNase H is a prototypical example for two metal ion catalysis in enzymes. An RNase H activity is present in the HIV-1 reverse transcriptase but also in many other nucleases such as Homo sapiens (Hs) or Escherichia coli (Ec) RNase H.
Yihan, Shao, Pablo G., Jambrina, Olga, Bohuszewicz, Reynier, Suardiaz, Christine, Peter, Edina, Rosta, Simon L., Dürr   +6 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Identification of HIV-1 Reverse Transcriptase-Associated Ribonuclease H Inhibitors Based on 2-Hydroxy-1,4-naphthoquinone Mannich Bases

Molecules
There is a strong demand for new and efficient antiviral compounds. A series of 2-hydroxy-1,4-naphthoquinone Mannich bases were screened for their HIV-1-RNase H inhibitory activity.
Nhat Quang Tu, Clémence Richetta, Federica Putzu, Olivier Delelis, Khursheed Ahmed, Vijay H. Masand, Rainer Schobert, Enzo Tramontano, Angela Corona, Bernhard Biersack   +9 more
doaj   +1 more source

Fluorinated Nucleotide Modifications Modulate Allele Selectivity of SNP-Targeting Antisense Oligonucleotides

Molecular Therapy: Nucleic Acids, 2017
Antisense oligonucleotides (ASOs) have the potential to discriminate between subtle RNA mismatches such as SNPs. Certain mismatches, however, allow ASOs to bind at physiological conditions and result in RNA cleavage mediated by RNase H.
Michael E. Østergaard, Josh Nichols, Timothy A. Dwight, Walt Lima, Michael E. Jung, Eric E. Swayze, Punit P. Seth   +6 more
doaj   +1 more source

Expression, Purification and Characterization of a Soluble and Active RNAse H from the Hepatitis B Virus

, 2007
The HBV RNAse H has been cloned into the PET43a vector, which contains the NusA protein which works as a solubilizing fusion protein. The fusion NUS-RNAse H protein was cleaved by enterokinase; the cleaved RNAse H is about 17 Kda which remains soluble ...
Saavedra, Mario Alejandro
core   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

N-Hydroxypiridinedione: A Privileged Heterocycle for Targeting the HBV RNase H [PDF]

Molecules
Hepatitis B virus (HBV) remains a global health threat. Ribonuclease H (RNase H), part of the virus polymerase protein, cleaves the pgRNA template during viral genome replication.
Moianos D, Makri M, Prifti G, Chiotellis A, Pappas A, Woodson M, Tajwar R, Tavis J, Zoidis G.   +8 more
europepmc   +1 more source

Selective inhibition of RNase H by dextran.

Journal of Biological Chemistry, 1981
Ordinarily, ribonuclease H hydrolyzes poly(rA) . poly(dT) and phiX174DNA-RNA at equal rates. Here we show that in the presence of dextran, the degradation of poly(rA) . poly(dT) is inhibited, while that of phi 174DNA-RNA is not. A similar inhibition by sucrose is found to be due to trace contamination of dextran in the sucrose. Ribose, deoxyribose, and
M L, Dirksen, R J, Crouch
openaire   +2 more sources