Results 61 to 70 of about 11,506,565 (250)

The Smc5/6 complex regulates the yeast Mph1 helicase at RNA-DNA hybrid-mediated DNA damage. [PDF]

PLoS Genetics, 2017
RNA-DNA hybrids are naturally occurring obstacles that must be overcome by the DNA replication machinery. In the absence of RNase H enzymes, RNA-DNA hybrids accumulate, resulting in replication stress, DNA damage and compromised genomic integrity.
Juan Lafuente-Barquero, Sarah Luke-Glaser, Marco Graf, Sonia Silva, Belén Gómez-González, Arianna Lockhart, Michael Lisby, Andrés Aguilera, Brian Luke   +8 more
doaj   +1 more source

The Dual Role of Histidine as General Base and Recruiter of a Third Metal Ion in HIV-1 RNase H [PDF]

, 2019
RNase H is a prototypical example for two metal ion catalysis in enzymes. An RNase H activity is present in the HIV-1 reverse transcriptase but also in many other nucleases such as Homo sapiens (Hs) or Escherichia coli (Ec) RNase H.
Yihan, Shao, Pablo G., Jambrina, Olga, Bohuszewicz, Reynier, Suardiaz, Christine, Peter, Edina, Rosta, Simon L., Dürr   +6 more
core   +2 more sources

KDAC6 alters cell morphology and motility through positive and negative modulation of F‐actin distribution

FEBS Open Bio, EarlyView.
KDAC6 has been associated with cell motility and actin structures, but specific domain contributions are not established. The two catalytic domains have differential effects on motility and F‐actin regulation, affecting cortical F‐actin density, motility, stress fibers, and cell spreading.
Taylor V. Joseph, Andrea B. Pham, Kiara E. Bornes, Razan H. Hammad, Chelsea A. Rousseve‐Ross, Thomas M. Huckaba, Terry J. Watt, Tasha B. Toro   +7 more
wiley   +1 more source

Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins

BMC Evolutionary Biology, 2010
Background The unfolding speed of some hyperthermophilic proteins is dramatically lower than that of their mesostable homologs. Ribonuclease HII from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-RNase HII) is stabilized by its remarkably
Koga Yuichi, Chon Hyongi, You Dong-Ju, Tadokoro Takashi, Mukaiyama Atsushi, Okamoto Tomohiro, Okada Jun, Takano Kazufumi, Kanaya Shigenori   +8 more
doaj   +1 more source

Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. [PDF]

PLoS ONE, 2014
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs.
Timothy A Vickers, Stanley T Crooke
doaj   +1 more source

1',5'-anhydrohexitol oligonucleotides: Hybridisation and strand displacement with oligoribonucleotides, interaction with RNase H and HIV reverse transcriptase

, 1997
Hexitol nucleic acids (HNAs) with four natural bases form stable and sequence-selective duplexes with RNA. This was investigated by T-m determinations and gel shift experiments. The CD spectra of an HNA-RNA duplex show similarities with the CD spectra of
Chris Hendrix, Herdewijn, Piet, Seela, F, Herdewijn, P., Van Aerschot, Arthur, Helmut Rosemeyer, Rosemeyer, H., Seela, Frank, Piet Herdewijn, Rosemeyer, H, Arthur Van Aerschot, Vanaerschot, A., Hendrix, Christel, Bart De Bouvere, DeBouvere, B, Frank Seela, De Bouvere, B., Hendrix, C   +17 more
core   +1 more source

Accurate and noninvasive prostate cancer detection using plasma‐derived extracellular vesicle RNA

FEBS Open Bio, EarlyView.
Plasma extracellular vesicles were captured with WGA‐conjugated magnetic beads and profiled for RNA biomarkers. A three‐RNA panel (NM_024955, NR_047469, and NR_002564) distinguished prostate cancer from healthy controls and benign prostatic hyperplasia, supporting a simple, noninvasive approach to improve prostate cancer detection.
Hanping Wei, Haoran Wu, Wei Feng
wiley   +1 more source

Enzymatically Inactive Virion-Incorporated HA-A3G Is Activated by Viral RNase H

, 2013
(A) Recombinant RTs containing either a WT or mutant (E478Q) RNase H catalytic domain were assessed for RNase H activity in vitro in the absence or presence of the RNase H inhibitor Compound I (final concentration of 1, 10, or 100 μM).
Warner C Greene (67961), Vanessa B Soros (68560), Wes Yonemoto (68561)   +2 more
core   +1 more source

Identification of (20R)‐protopanaxadiol from Panax ginseng as a novel anti‐SARS‐CoV‐2 compound

FEBS Open Bio, EarlyView.
We established a noninfectious BAC‐based SARS‐CoV‐2 replicon that enables antiviral screening under BSL‐2 conditions. Using this platform, we screened 373 food‐derived compounds and identified (20R)‐protopanaxadiol from Panax ginseng as a novel inhibitor of SARS‐CoV‐2 RNA replication, highlighting the value of safe replicon systems for antiviral ...
Midori Takeda, Natsue Maezono, Rina Uchikoshi, Nobuyuki Kato, Masanori Ikeda   +4 more
wiley   +1 more source

2′β-Fluoro-Tricyclo Nucleic Acids (2′F-tc-ANA): Thermal Duplex Stability, Structural Studies, and RNase H Activation [PDF]

, 2017
We describe the synthesis, thermal stability, structural and RNase H activation properties of 2′β-fluoro-tricyclo nucleic acids (2′F-tc-ANA). Three 2′F-tc-ANA nucleosides (T, 5MeC and A) were synthesized starting from a previously described fluorinated ...
Adam Katolik, Istrate, Andrei, Leumann, Christian, Katolik, Adam, Alena Istrate, Istrate, Alena, Andrei Istrate, Christian J. Leumann   +7 more
core   +4 more sources