Results 71 to 80 of about 303,152 (296)

Identification of highly conserved residues involved in inhibition of HIV-1 RNase H function by Diketo acid derivatives [PDF]

, 2014
HIV-1 reverse transcriptase (RT)-associated RNase H activity is an essential function in viral genome retrotranscription. RNase H is a promising drug target for which no inhibitor is available for therapy.
Di Leva F. S, Corona A, Rigogliuso G, Delelis O, Thierry S, Novellino E, TRAMONTANO, ENZO, ESPOSITO, FRANCESCA, Subra F, Cosconati S, CORONA, ANGELA, Costi R, Esposito F, Pescatori L, Di Leva FS, Di Santo R, Tramontano E., COSCONATI, Sandro, Cuzzucoli Crucitti G   +18 more
core   +2 more sources

Evolution and thermodynamics of the slow unfolding of hyperstable monomeric proteins

BMC Evolutionary Biology, 2010
Background The unfolding speed of some hyperthermophilic proteins is dramatically lower than that of their mesostable homologs. Ribonuclease HII from the hyperthermophilic archaeon Thermococcus kodakaraensis (Tk-RNase HII) is stabilized by its remarkably
Koga Yuichi, Chon Hyongi, You Dong-Ju, Tadokoro Takashi, Mukaiyama Atsushi, Okamoto Tomohiro, Okada Jun, Takano Kazufumi, Kanaya Shigenori   +8 more
doaj   +1 more source

Antisense oligonucleotides capable of promoting specific target mRNA reduction via competing RNase H1-dependent and independent mechanisms. [PDF]

PLoS ONE, 2014
Antisense oligonucleotides (ASOs) are most commonly designed to reduce targeted RNA via RNase H1-dependent degradation. In this paper we demonstrate that cellular proteins can compete for sites targeted by RNase H1-dependent ASOs.
Timothy A Vickers, Stanley T Crooke
doaj   +1 more source

Improvement of Reverse Transcription PCR by RNase H

Bioscience, Biotechnology, and Biochemistry, 2003
An improvement in the method of the Reverse Transcription PCR (RT-PCR) using RNase H is proposed here. We succeeded in RT-PCR amplification against the full sequence of the coding region (8.9 kb) of the Insulin-like growth factor II receptor gene which has the area called the GC-block of about 90% GC contents at the 5' terminal.
KITABAYASHI, Masao, ESAKA, Muneharu
openaire   +2 more sources

MITF maintains genome stability in nonmelanocyte lineages

Molecular Oncology, EarlyView.
MITF is essential for melanocyte survival and acts as an oncogene in 10%–20% of melanomas. We show that MITF depletion causes genome instability in nonmelanocytic cells, leading to LATS2‐mediated P53 activation, cell cycle arrest, and apoptosis. This study highlights the role of MITF as a genome maintenance factor beyond the melanocyte lineage. Created
Drifa H. Gudmundsdottir, Adrián López García de Lomana, Thejus B. Venkatesh, Kritika Kirty, Snaevar Sigurdsson, Linda Vidarsdottir, Ramile Dilshat, Erla Sveinbjornsdottir, Snaedis Ragnarsdottir, Daniel H Magnusson, Maria R. Bustos, Eirikur Steingrimsson, Thorkell Gudjonsson, Stefan Sigurdsson   +13 more
wiley   +1 more source

Structure and function of an RNase H domain at the heart of the spliceosome [PDF]

The EMBO Journal, 2008
Precursor-messenger RNA (pre-mRNA) splicing encompasses two sequential transesterification reactions in distinct active sites of the spliceosome that are transiently established by the interplay of small nuclear (sn) RNAs and spliceosomal proteins.
Pena, V., Rozov, A., Fabrizio, P., Luehrmann, R., Wahl, M.   +4 more
openaire   +4 more sources

Properties of structurally characterized RNase H homologs.

, 2013
Properties of the RNase H proteins and the crystal structures used to initiate the trajectories described herein. RMSD and percent sequence identity are relative to ecRNH.and, not determined.
Paul Robustelli (466139), Kate A. Stafford (466138), Arthur G. Palmer III (466140)   +2 more
core   +1 more source

Preparation and characterization of the RNase H domain of Moloney murine leukemia virus reverse transcriptase. [PDF]

, 2015
Moloney murine leukemia virus reverse transcriptase (MMLV RT) contains fingers, palm, thumb, and connection subdomains as well as an RNase H domain. The DNA polymerase active site resides in the palm subdomain, and the RNase H active site is located in ...
Nishimura, Kosaku, Hisayoshi, Tetsuro, Yokokawa, Kanta, Kojima, Kenji, Fukatsu, Kosuke, Mikami, Bunzo, Konishi, Atsushi, Kuze, Ikumi, Yasukawa, Kiyoshi   +8 more
core   +1 more source

Liquid biopsy‐based diagnostic evaluation of hypermethylated CpG sites for ovarian cancer diagnosis

Molecular Oncology, EarlyView.
This schematic outlines the workflow from biomarker identification to duplex MethyLight assay validation for epithelial ovarian cancer diagnosis using cfDNA‐based liquid biopsy. Initial screening of hypermethylated CpG candidates (cg02957270, cg10061138 cg00480298, COL2A1) was performed in tissue using ARMS‐PCR, COBRA, qPCR and image analysis. Selected
Deepa Bisht, Sameer Gupta, Amrita Chaurasia, Manisha Sachan   +3 more
wiley   +1 more source

RNase H and replication of ColE1 DNA in Escherichia coli [PDF]

Journal of Bacteriology, 1986
Amber mutations within the rnh (RNase H) gene of Escherichia coli K-12 were isolated by selecting for bacteria capable of replicating in a sup+ background replication-defective cer-6 mutant of the ColE1 replicon. The cer-6 mutation is an alteration of one base pair located 160 nucleotides upstream of the unique replication origin of this plasmid ...
S, Naito, H, Uchida
openaire   +2 more sources