Results 111 to 120 of about 303,152 (296)
RNase H activity of HIV reverse transcriptases is confined exclusively to the dimeric forms
, 1992 A method for the rapid preparation of a defined substrate to monitor RNase H activity has been developed. Using this substrate, we have investigated the RNase H activities of the different forms of recombinant HIV−1 and HIV−2 reverse, transcriptase (RT ...
Goody, R.S. +9 more
core +1 more source
Advanced Materials, EarlyView.Inspired by viral entry mechanisms, the FUSION assay enables autonomous detection of respiratory viruses via membrane fusion–triggered CRISPR‐Cas13a activation. VEACON selectively fuses with fusion‐competent viruses, triggering fluorescence within confined vesicles.
Jae Chul Park +15 more
wiley +1 more source
Advanced Materials, EarlyView.The intimate interaction between negatively charged RNA molecules and conjugated polymers in organic electrochemical transistors (OECTs) in aqueous electrolytes is investigated. It demonstrates that RNA binding reduces the volumetric capacitance of the polymer channel, enabling the development of an ultrasensitive biosensing platform capable of ...
Hong Liu +7 more
wiley +1 more source
Full-Length cDNA Synthesis for Long-Distance RT-PCR of Large mRNA Transcripts
BioTechniques, 2003 Reverse transcription of mRNA often leads to the synthesis of partial, non-fulllength cDNAs. Methods to facilitate reverse transcription across RNA regions of secondary structure, as well as enzyme modifications to eliminate RNase H activities inherent ...
Phillip R. Hawkins, Pei Jin, Glenn K. Fu
doaj +1 more source
QBP1 Peptide as a Potential Anti‐Amyloidogenic Therapy for Type 2 Diabetes: An In Vitro Study
Advanced Science, EarlyView.The anti‐amyloidogenic peptide QBP1 effectively halts human islet amyloid polypeptide (hIAPP) aggregation, preventing the formation of toxic β‐structured intermediates. Through a combination of biophysical assays, molecular dynamics, and cell‐based studies, QBP1 is shown to preserve β‐cell viability and metabolic homeostasis, positioning it as a ...
María M. Tejero‐Ojeda +8 more
wiley +1 more source
Detection of Protein–Protein Interactions in Escherichia coli With Single Molecule Sensitivity
Advanced Science, EarlyView.This article describes a regulatory circuit in Escherichia coli able to detect protein–protein interactions with exquisite sensitivity. The interaction between two hybrid proteins fused to Bordetella pertussis adenylate cyclase and its activator calmodulin triggers a potent cyclic AMP signaling cascade.
Marilyne Davi, Daniel Ladant
wiley +1 more source
Expression of an Mg 2+ -Dependent HIV-1 RNase H Construct for Drug Screening
, 2011 A single polypeptide of the HIV-1 reverse transcriptase that reconstituted Mg 2+ -dependent RNase H activity has been made. Using molecular modeling, the construct was designed to encode the p51 subunit joined by a linker to the ...
Monica J. Roth +6 more
core +1 more source
Advanced Science, EarlyView.We report a new thiolate‐reactive α,α‐gem‐dibromo lactam warhead that activates transcription factor Nrf2 and demonstrates anti‐inflammatory activities, which have implications in cancer, neurodegeneration, and cardiovascular diseases. RNA‐seq illuminated detailed transcriptional profiles, and chemical reactions with cysteine‐containing compounds ...
Beau R. Brummel +16 more
wiley +1 more source
C-terminal RNase H Domain of Hepatitis B Virus Polymerase Is Essential for HBV DNA synthesis
, 2014 Multi-functional polymerase (P) protein of hepatitis B virus (HBV) functions in pregenomic RNA (pgRNA) encapsidation, minus-strand DNA initiation, and DNA synthesis.
Jung, Jaesung +2 more
core
, 2015 Cellular RNase H domains used here are identical to those in [34]. Numbers at nodes indicate bootstrap values of 100 replicates; only values over 50 are shown. We show bootstrap values and aLRT values at 5 nodes. The RNase H domains of Utopia are colored
Jerzy Jurka (9176) +1 more
core +1 more source