Results 131 to 140 of about 303,152 (296)

PRMT1‐Mediated LDHA Methylation Drives STAT3 Lactylation to Orchestrate Intestinal Inflammation and Tumorigenesis

Advanced Science, EarlyView.
This study identifies an immunometabolic axis wherein SAM‐driven PRMT1 methylates LDHA, enhancing its activity. The resultant lactate induces STAT3 K709 lactylation, which stabilizes an active conformation to promote STAT3 phosphorylation and IL‐10 expression.
Hui Wang, Mengyu Zhang, Weipeng Gong, Jiaxuan Wu, Junping Zhang, Wenxiu Zhang, Yue Liu, Kui Wang, Canhua Huang, Jun Zhou, Sijin Wu, Yan Li, Tianliang Li   +12 more
wiley   +1 more source

RNA Helicase DDX21 Controls CD4+ T Cell Proliferation and Promotes Inflammatory Bowel Disease via Translational Control

Advanced Science, EarlyView.
ABSTRACT Inflammatory bowel disease (IBD) is characterized by dysregulated T cell responses. RNA helicases, including DExD‐box helicase 21 (DDX21), are pivotal in RNA metabolism, but their role in T cell‐mediated pathology during IBD remains unclear. Here, we demonstrate that DDX21 expression in CD4+ T cells correlates with cell cycle and translation ...
Yujuan Zhang, Chen Kan, Xinhui Yang, Yajuan Hao, Xuemin Cai, Mei Yang, Qianqian Zhang, Zhengting Wang, Ning Jiang, Lei Wang, Hua‐Bing Li, Jing Zhou   +11 more
wiley   +1 more source

Expression of thermostable MMLV reverse transcriptase in Escherichia coli by directed mutation

AMB Express
The functionality of Moloney murine leukemia virus reverse transcriptase (MMLV RT) will increase with the improvement of its solubility and thermal stability.
Marzieh Divbandi, Ahad Yamchi, Hadi Razavi Nikoo, Abdolvahab Moradi, Alijan Tabarraei   +4 more
doaj   +1 more source

RNase H genes cause distinct impacts on RNA:DNA hybrid formation and mutagenesis genome wide. [PDF]

Sci Adv, 2023
Schroeder JW, Hurto RL, Randall JR, Wozniak KJ, Timko TA, Nye TM, Wang JD, Freddolino L, Simmons LA.   +8 more
europepmc   +1 more source

Determining the enzyme activity of Endo H-P through mobility shift assay of RNase B.

, 2015
M protein molecular weight markers (the size of each band was indicated on the left); Lane 1 denatured RNase B (negative control); Lane 2 denatured RNase B treated with 1 U of commercial Endo H from NEB, USA (positive control); Lane 3–8 denatured RNase B
Xiaolan Yu (703277), Lixin Ma (18620), Xiaojuan Wang (105141), Chao Zhai (324088), Yunyun Liu (538796), Lin Fu (320968), Fei Wang (19534)   +6 more
core   +1 more source

Canagliflozin Alleviates Diabetic Glomerular Endothelial Injury via Melibiose in a Microbiota‐Dependent Manner

Advanced Science, EarlyView.
Canagliflozin treatment reshapes the gut microbiota in DKD and elevates levels of melibiose, a metabolite derived from Roseburia intestinalis. Melibiose directly binds to and enhances the enzymatic activity of glyoxalase 1, leading to decreased methylglyoxal accumulation.
Wei Zhang, Yi Song, Changkun Li, Yuanyuan Luo, Mingwei Shao, Feng Guo, Fangyi Wei, Xunjie Fan, Wenwen Guo, Fengmei Xu, Yanhong Sang, Dongming Zhang, Yanhong Zhou, Lianwei Wang, Zhiqiang Kang, Yingjun Yang, Chunhua Song, Yanxia Liu, Xiaojun Ma, Jiao Wang, Chong Li, Shengnan Ma, Lin Zhao, Zhi Qin, Guolan Xing, Qiubo Zhao, Jun Li, Shumin Song, Dan Zhao, Ting Huang, Qingzhu Wang, Yanyan Zhao, Guijun Qin   +32 more
wiley   +1 more source

RNase H-based analysis of synthetic mRNA 5' cap incorporation. [PDF]

RNA, 2022
Chan SH, Whipple JM, Dai N, Kelley TM, Withers K, Tzertzinis G, Corrêa IR, Robb GB.   +7 more
europepmc   +1 more source

HIV-1 RNase H: Recent Progress in an Exciting, yet Little Explored, Drug Target

, 2006
HIV-1 reverse transcriptase-associated ribonuclease H (RNase H) is an attractive non traditional target for drug development which has been, so far, little explored.
TRAMONTANO, ENZO
core   +1 more source

A Radioresistant‐Tumor‐Targeted Nanoparticle for X‐Ray‐Controlled Nitric Oxide Release to Potentiate Radiotherapy

Advanced Science, EarlyView.
The PBTN targets GRP78 and, upon X‐ray irradiation, generates ONOO− to induce immunogenic cell death, thereby activating CD8+ T cells and establishing a systemic antitumor immune response. ABSTRACT Nitric oxide (NO) treated radioresistant tumors by relieving hypoxia and blocking DNA repair, but its nonselective toxicity has precluded therapeutic use ...
Wanze Zhang, Xiaoyan Yin, Ting Wang, Hongfu Zhao, Zhipeng Zhao, Jinbao Wang, Cheng Tao, Xuanchu Ge, Yanze Li, Linlin Liu, Fuxin Xue   +10 more
wiley   +1 more source

RNase H Active Site Inhibitors of Human Immunodeficiency Virus Type 1 Reverse Transcriptase: Design, Biochemical Activity, and Structural Information

, 2016
Pyrimidinol carboxylic acids were designed as inhibitors of HIV-1 RNase H function. These molecules can coordinate to two divalent metal ions in the RNase H active site.
Katherine M. Brendza (158359), John Mak (2345713), Neil H. Squires (2345719), Tiffany Barnes (549800), Xiaowu Chen (212229), Nilima Kutty (2296144), William J. Watkins (508002), Mini Balakrishnan (456383), Michael Wang (265591), Eugene J. Eisenberg (2160961), Xiaohong Liu (50816), Jason K. Perry (2345716), Stephanie Leavitt (385640), Albert Liclican (2345725), Thorsten A. Kirschberg (2345710), Qi Liu (33068), Eric B. Lansdon (2296147), Weili Jin (2345722)   +17 more
core   +1 more source