Results 131 to 140 of about 14,336,830 (357)

RNA Sequencing of Formalin-Fixed, Paraffin-Embedded Specimens for Gene Expression Quantification and Data Mining

International Journal of Genomics, 2016
Background. Proper rRNA depletion is crucial for the successful utilization of FFPE specimens when studying gene expression. We performed a study to evaluate two major rRNA depletion methods: Ribo-Zero and RNase H.
Yan Guo, Jie Wu, Shilin Zhao, Fei Ye, Yinghao Su, Travis Clark, Quanhu Sheng, Brian Lehmann, Xiao-ou Shu, Qiuyin Cai   +9 more
doaj   +1 more source

Synthetic Strategy for mRNA Encapsulation and Gene Delivery with Nanoscale Metal‐Organic Frameworks

Advanced Functional Materials, EarlyView.
This research utilizes the ZIF‐8 for the encapsulation and intracellular delivery of nucleic acids, specifically mRNA, for applications in gene delivery. Integrating PEI addresses the issue of mRNA leakage from ZIF‐8, resulting in the delivery and expression of green fluorescent protein (GFP) in vitro and firefly luciferase in vivo.
Harrison Douglas Lawson, Huy Hoang Nguyen, Keng‐Jung Lee, Nattarat Wongsuwan, Ayesha Tupe, Mengrou Lu, Mariah Lynn Arral, Anne Behre, Zihan Ling, Kathryn Ann Whitehead, Adam Walter Feinberg, Xi Ren, Si‐Yang Zheng   +12 more
wiley   +1 more source

Complexities associated with expression of Epstein-Barr virus (EBV) lytic origins of DNA replication. [PDF]

, 2007
EBV has two lytic origins (oriLyt) of DNA replication lying at divergent sites on the viral genome within a duplicated sequence (DS). The latter contains potential hairpin loops, ‘hinge’ elements and the promoters for transcripts from viral genes BHLF1 ...
Griffin, BE, Xue, SA
core   +2 more sources

Endocytic Programming via Porous Silicon Nanoparticles Enhances TLR4 Nanoagonist Potency for Macrophage‐Mediated Immunotherapy

Advanced Functional Materials, EarlyView.
Porous silicon nanoparticles (PSiNPs) reprogram macrophage endocytosis of manganese@albumin‐based TLR4 nanoagonists, driving TRIF‐biased TLR4 signaling, eliciting robust proinflammatory responses, and potentiating macrophage‐mediated immunotherapeutic effects against NSCLC.
Xiaomei Zhang, Huiying Li, Shuodan Huang, Linxuan Zhang, Yan Gao, Ruoran Wang, Xiaoyu Wang, Hélder A. Santos, Zhenyu Yin, Bing Xia   +9 more
wiley   +1 more source

DNA-based S-genotyping of Japanese plum and pluot cultivars to clarify incompatibility relationships [PDF]

, 2007
Diploid japanese plum (Prunus salicina Lindl.) cultivars are commonly self-incompatible. To date, 14 incompatibility alleles (S-alleles) have been identified and labeled with alphabetical (S-a-S-n) and 5 with numeric codes (S-1, S-3-S-6).
Halász, Júlia, Hegedűs, Attila, Pedryc, Andrzej, Szabó, Zoltán   +3 more
core   +1 more source

Cholesterol in mRNA‐Lipid Nanoparticles can be Replaced with the Synthetic Mycobacterial Monomycoloyl Glycerol Analogue MMG‐1

Advanced Functional Materials, EarlyView.
This study demonstrates that cholesterol in messenger RNA‐lipid nanoparticles (mRNA‐LNPs) can be completely replaced with an immunopotentiating lipid, i.e., a synthetic analogue of the C‐type lectin receptor agonist monomycoloyl glycerol (MMG‐1), without compromising physicochemical properties, in vivo transfection efficiency, and immunogenicity of the
Abhijeet G. Lokras, Saahil S. Baghel, Rune F. Jensen, Aneesh Thakur, Henrik Franzyk, Oskar Thofte, Marco Herrera‐Barrera, Madeleine Landry, Melike Ongun, Ahmad Tami, Marlene P. Thanthrige, Eva Callens, Corinne Beinat, Thomas Rades, Søren R. Paludan, Kristian Riesbeck, Ida Rosenkrands, Dennis Christensen, Gabriel K. Pedersen, Camilla Foged   +19 more
wiley   +1 more source

Broad role for YBX1 in defining the small noncoding RNA composition of exosomes. [PDF]

, 2017
RNA is secreted from cells enclosed within extracellular vesicles (EVs). Defining the RNA composition of EVs is challenging due to their coisolation with contaminants, lack of knowledge of the mechanisms of RNA sorting into EVs, and limitations of ...
Lambowitz, Alan M, Nottingham, Ryan M, Qin, Yidan, Schekman, Randy, Shurtleff, Matthew J, Temoche-Diaz, Morayma M, Yao, Jun   +6 more
core   +1 more source

Metal Nanoclusters for Cancer Imaging and Treatment

Advanced Functional Materials, EarlyView.
This review aims to provide a comprehensive summary and discussion of the core–shell design capabilities of metal nanoclusters (NCs) at the atomic level for cancer imaging and treatment. It offers essential insights into the design principles of metal NCs while also encouraging the exploration of other nanomaterials and their potential theranostic ...
Haiguang Zhu, Ziping Wang, Qiaofeng Yao, Ping Zhang, Jianping Xie, Xun Yuan   +5 more
wiley   +1 more source

RNase Y mediates posttranscriptional control of the virulence-associated CncR1 small-RNA in Helicobacter pylori

iScience
Summary: Ribonucleases are involved in several biological processes, including the turnover of structural and messenger RNAs and the specific processing of the cellular transcriptome. Here, we characterized the RNase Y from Helicobacter pylori.
Federico D’Agostino, Eva Pinatel, Alexandra Meynhardt, Vincenzo Scarlato, Andrea Vannini, Davide Roncarati   +5 more
doaj   +1 more source

Comparative evaluation for the globin gene depletion methods for mRNA sequencing using the whole blood-derived total RNAs

BMC Genomics, 2020
Background There are challenges in generating mRNA-Seq data from whole-blood derived RNA as globin gene and rRNA are frequent contaminants. Given the abundance of erythrocytes in whole blood, globin genes comprise some 80% or more of the total RNA ...
Jin Sung Jang, Brianna Berg, Eileen Holicky, Bruce Eckloff, Mark Mutawe, Minerva M. Carrasquillo, Nilüfer Ertekin-Taner, Julie M. Cuninngham   +7 more
doaj   +1 more source