Results 251 to 260 of about 303,152 (296)

An RNase H-powered DNA walking machine for sensitive detection of RNase H and the screening of related inhibitors

Nanoscale, 2020
A signal amplification strategy based on an RNase H-powered DNA walking machine for RNase H activity detection.
Yafang Wang, Namin Hu, Chang Liu, Cunpeng Nie, Manman He, Juan Zhang, Qiaoqin Yu, Chuan Zhao, Tingting Chen, Xia Chu   +9 more
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The RNase H Mechanism

2007
Antisense oligonucleotides (ASOs) are designed to modulate the information transfer from gene to protein-in essence to alter mRNA intermediary metabolism. mRNA intermediary metabolism is extremely complex beginning with transcription and concluding with degradation usually after translation.
Hongjiang Wu, Stanley Crooke, Walt Lima
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Function of RNase H in DNA replication revealed by RNase H defective mutants of Escherichia coli

Molecular and General Genetics MGG, 1984
Escherichia coli rnh mutants were isolated using localized mutagenesis and selective measurements of RNase H activity in mutagenized cell extracts with [3H]poly(rC) X poly(dG) as substrate. RNase H activity in extracts of one mutant, ON152 (rnh-91), was undetectable (less than 0.05% of that of wild-type cells).
T, Ogawa, T, Okazaki
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Stereochemical Course of Escherichia coli RNase H.

ChemInform, 2002
A new enzymatic method has allowed the assignment of the stereochemistry of E. coli RNase-H-assisted hydrolysis of RNA labelled within the scissile bond with (R(p))-phosphorothioate. This method is based on a stereospecific, two-step enzymatic conversion of cytidine 5'-[(18)O]phosphorothioate into the corresponding 5'-alpha-[(18)O]thiotriphosphate ...
Agnieszka, Krakowiak, Alina, Owczarek, Maria, Koziołkiewicz, Wojciech J, Stec   +3 more
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Discrimination between mammalian RNases H-1 and H-2

Analytical Biochemistry, 1991
The two principal RNases H in mammalian cells, H-1 and H-2, differ in their responses to sale, divalent metal, and sulfhydryl inhibition. Specific reaction conditions that provide unambiguous discrimination between RNases H-1 and H-2 with only two assays are described. The assays were used for identification in a new purification procedure for RNases H-
M, Goulian, C J, Heard
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Pyridopyrimidinone inhibitors of HIV-1 RNase H

European Journal of Medicinal Chemistry, 2014
Using a structure based pharmacophore design, a weak inhibitor of RNase H, identified from a small library of two metal binding HIV-1 integrase inhibitors, was optimized for potency and physicochemical properties. This manuscript describes the SAR and in vivo DMPK for the pyridopyrimidinone class of inhibitors.
Emile J. Velthuisen, Brian A. Johns, Peter Gerondelis, Yan Chen, Ming Li, Ke Mou, Wenwen Zhang, John W. Seal, Kendra E. Hightower, Sonia R. Miranda, Kevin Brown, Lisa Leesnitzer   +11 more
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Boranophosphates Support the RNase H Cleavage of Polyribonucleotides

Antisense and Nucleic Acid Drug Development, 1999
Modification of the phosphodiester linkages in DNA by replacing one of the nonbridging oxygens with borane, BH3, produces an isoelectronic mimic of DNA called boranophosphates. Nonstereoregular oligodeoxyribonucleoside all-boranophosphates are shown here for the first time to elicit the RNase H hydrolysis of polyribonucleotides. We compared the ability
V K, Rait, B R, Shaw
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RNase H Activation by Stereoregular Boranophosphate Oligonucleotide

ChemInform, 2003
AbstractFor Abstract see ChemInform Abstract in Full Text.
Xin, Wang, Mikhail, Dobrikov, Dmitri, Sergueev, Barbara Ramsay, Shaw   +3 more
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RNase H-defective mutants of Escherichia coli: A possible discriminatory role of RNase H in initiation of DNA replication

Molecular and General Genetics MGG, 1984
Mutants of Escherichia coli completely deficient in RNase H activity were isolated by inserting transposon Tn3 into the structural gene for RNase H, rnh, and its promoter. These rnh- mutants exhibited the following phenotypes; (1) the mutants grew fairly normally, (2) rnh- cells could be transformed with ColE1 derivative plasmids, pBR322 and pML21 ...
T, Horiuchi, H, Maki, M, Sekiguchi
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A large deletion in the connection subdomain of murine leukemia virus reverse transcriptase or replacement of the RNase H domain with Escherichia coli RNase H results in altered polymerase and RNase H activities

Biochemistry, 1993
The functional relationship between the polymerase and RNase H domains of reverse transcriptase (RT) was investigated by studying the activities of AKR murine leukemia virus (MuLV) enzymes. In addition to the wild type, an RNase H-minus RT missing the entire RNase H domain and two other mutants having abnormal polymerase:RNase H ratios were expressed ...
K, Post, J, Guo, E, Kalman, T, Uchida, R J, Crouch, J G, Levin   +5 more
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