Results 111 to 120 of about 321,082 (269)

OTIMIZAÇÃO DA TÉCNICA DA PCR PARA A DETECÇÃO DO POLIMORFISMO CYP3A4*1B E FREQUÊNCIA ALÉLICA ENTRE 60 MORADORES DE SÃO LUÍS/MA

Cadernos de Pesquisa, 2013
Os objetivos deste trabalho foram adequar a técnica de PCR para o polimorfismo CYP3A4*1B, reduzir as amplificações inespecíficas observadas e verificar a frequência alélica do polimorfismo na população de São Luís/MA. A reação de PCR foi padronizada para
Bruna Aparecida Penha, Israel Higino de Sousa, Wandson Rodrigues Sousa, Jomar Diogo Nunes, Marcelo dos Santos, Sally Cristina Moutinho Monteiro   +5 more
doaj   +1 more source

Hfq binding changes the structure of Escherichia coli small noncoding RNAs OxyS and RprA, which are involved in the riboregulation of rpoS [PDF]

, 2013
OxyS and RprA are two small noncoding RNAs (sRNAs) that modulate the expression of rpoS, encoding an alternative sigma factor that activates transcription of multiple Escherichia coli stress-response genes. While RprA activates rpoS for translation, OxyS
Callaghan, Anastasia, Cary, Peter, Casamento, A., Gowers, Darren, Henderson, Charlotte, Phillips, Jack, Sobott, F., Stone, Carlanne, Taylor, James E., Vincent, H.   +9 more
core   +1 more source

Proteasomal degradation of intracellularly expressed Amblyomin‐X limits suicide gene therapy potential in melanoma cells

FEBS Open Bio, EarlyView.
This study explores the feasibility of expressing the antitumoral protein Amblyomin‐X through a suicide gene therapy approach and investigates its intracellular fate after gene delivery. Although the gene is efficiently expressed, melanoma cells rapidly degrade the Amblyomin‐X protein via proteasome activity.
Victor Dal Posolo Cinel, Carlos DeOcesano Pereira, Aline Ramos Maia Lobba, Melissa Regina Fessel, Ana Marisa Chudzinski‐Tavassi   +4 more
wiley   +1 more source

Myxoma virus protein M029 is a dual function immunomodulator that inhibits PKR and also conscripts RHA/DHX9 to promote expanded host tropism and viral replication. [PDF]

, 2013
Myxoma virus (MYXV)-encoded protein M029 is a member of the poxvirus E3 family of dsRNA-binding proteins that antagonize the cellular interferon signaling pathways.
Chan, Winnie M, Liu, Jia, McFadden, Grant, Rahman, Masmudur M, Rothenburg, Stefan   +4 more
core   +1 more source

Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system

FEBS Open Bio, EarlyView.
An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková, Veronika Hovanová, Jiri Damborsky, Erik Sedlák   +3 more
wiley   +1 more source

Hfq Globally Binds and Destabilizes sRNAs and mRNAs in Yersinia pestis

mSystems, 2019
Hfq is a ubiquitous Sm-like RNA-binding protein in bacteria involved in physiological fitness and pathogenesis, while its in vivo binding nature remains elusive. Here we reported genome-wide Hfq-bound RNAs in Yersinia pestis, a causative agent of plague,
Yanping Han, Dong Chen, Yanfeng Yan, Xiaofang Gao, Zizhong Liu, Yaqiang Xue, Yi Zhang, Ruifu Yang   +7 more
doaj   +1 more source

Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10

FEBS Open Bio, EarlyView.
Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee, Ga Seul Lee, JinHan Kim, Kwang‐Hyun Park, Seong‐Ryung Go, Jeong Hee Moon, Eui‐Jeon Woo   +6 more
wiley   +1 more source

Attenuation of RNA polymerase II pausing mitigates BRCA1-associated R-loop accumulation and tumorigenesis. [PDF]

, 2017
Most BRCA1-associated breast tumours are basal-like yet originate from luminal progenitors. BRCA1 is best known for its functions in double-strand break repair and resolution of DNA replication stress.
Angel Pujana, Miguel, Brunet, Joan, Chaldekas, Krysta, Chedin, Frederic, Chiang, Huai-Chin, Curiel, Tyler J, Elledge, Richard, Hu, Yanfen, Isaacs, Claudine, Jatoi, Ismail, Jin, Victor X, Lautner, Meeghan, Li, Rong, Mateo, Francesca, Michalek, Joel, Nair, Sreejith J, Ochoa, Oscar, Oliver, Boyce, Peshkin, Beth N, Petit, Anna, Poggi, Elizabeth, Smith, Sabrina, Soler, Teresa, Sun, Lu-Zhe, Theoharis, Constantine, Wang, Howard, Wang, Yao, Zhang, Chi, Zhang, Xiaowen, Zhao, Xiayan   +29 more
core   +2 more sources

Mutant NPM1 in Acute Myeloid Leukemia Initiation and Maintenance

Aging and Cancer, EarlyView.
NPM1 mutations drive acute myeloid leukemia by acting as neomorphic transcriptional regulators that cooperate with Menin–MLL and XPO1 to sustain HOX/MEIS1 expression and block differentiation. Targeting these mutant‐specific transcriptional dependencies provides a rational therapeutic strategy for NPM1‐mutated AML.
Yanan Jiang, Zhong Fan, Runmeng Liu, Xiaotian Zhang   +3 more
wiley   +1 more source

RNA elements directing in vivo assembly of the 7SK/MePCE/Larp7 transcriptional regulatory snRNP [PDF]

, 2013
Through controlling the nuclear level of active positive transcription elongation factor b (P-TEFb), the 7SK small nuclear RNA (snRNA) functions as a key regulator of RNA polymerase II transcription.
Bayfield, Bhattacharya, Biewenga, Blazek, Chambers, Cheng, de Almeida, Diribarne, Egloff, Goodall, Gupta, Gupta, Jeronimo, Jobert, Kiss, Kiss, Kiss, L. Muniz, Markert, Michels, Muniz, Nguyen, O'Keeffe, Peterlin, Price, S. Egloff, Shimba, Shimba, Shumyatsky, Shumyatsky, Singh, Singh, Singh, T. Kiss, Takahasi, Van Herreweghe, Wada, Wassarman, Yamaguchi, Yang, Yik, Yik, Zhou, Zhou   +43 more
core   +3 more sources