Results 91 to 100 of about 93,670 (312)

Complexities of gammaherpesvirus transcription revealed by microarrays and RNAseq [PDF]

Current Opinion in Virology, 2013
Technological advances in genome-wide transcript analysis, referred to as the transcriptome, using microarrays and deep RNA sequencing methodologies are rapidly extending our understanding of the genetic content of the gammaherpesviruses (γHVs). These vast transcript analyses continue to uncover the complexity of coding transcripts due to alternative ...
openaire   +2 more sources

Single‐Cell Transcriptomic Profiling Identifies B Cell‐Intrinsic Dysregulation of Sphingolipid Biosynthesis and Could Be Improved by Inhibiting UGCG in Primary Immune Thrombocytopenia

Advanced Science, EarlyView.
This study generates the single‐cell atlas of pathogenic B‐cell subset Bc‐5 in ITP. It identifies RUNX3 as a key survival regulator and uncovers sphingolipid‐driven metabolic reprogramming, validating UGCG inhibition as a therapeutic candidate and building a valuable resource for ITP mechanistic and therapeutic exploration.
Dongmei Luo, Yanxia Zhan, Lili Ji, Pengcheng Xu, Pu Chen, Hao Pang, Yunan Ling, Fanli Hua, Xibing Zhuang, Hao Chen, Yunfeng Cheng   +10 more
wiley   +1 more source

The Avian RNAseq Consortium: a community effort to annotate the chicken genome [PDF]

, 2014
Here we describe how members of the chicken research community have come together as the “Avian RNAseq Consortium” to provide chicken RNAseq data with a view to improving the annotation of the chicken genome. The data was used by Ensembl in their release
Avian RNAseq Consortium, Burt, David, Smith, Jacqueline, Lamont, Susan   +3 more
core  

Online FDR Control for RNAseq Data

, 2022
Motivation: While the analysis of a single RNA sequencing (RNAseq) dataset has been well described in the literature, modern research workflows often have additional complexity in that related RNAseq experiments are performed sequentially over time.
Liou, Lathan, Hornburg, Milena, Robertson, David S.   +2 more
openaire   +2 more sources

Long‐Term Human Skin Platform for Modeling Chronic Inflammation, Environmental Stress, and Therapeutic Intervention

Advanced Science, EarlyView.
An advanced, long‐term human skin platform maintains native skin structure and key tissue functions for up to four weeks. The platform models chronic inflammation, aging‐related skin changes, and UVB damage, enabling drug and topical interventions—including anti‐inflammatory therapy, senolytic treatment, and sunscreen—to be evaluated in intact human ...
Purnendu Kumar Sharma, Evelien Schaafsma, Elysia Anderson, Jocelyn Chiari, Paige Thompson, Erin Galt, Sunghoon Lee, Jason Holsapple, Sai Hein, Bailey L. Coates, Jade Michaud, Sabrina Zuccaro, Lia Kent, Chris Hinojosa, Kyung‐Jin Jang   +14 more
wiley   +1 more source

Cell Surface Markers Identify Astrocyte Subpopulations in the Adult Hippocampus With a Heterogeneous Response to Aging. [PDF]

Glia
ATP1B2 and GLAST identify two astrocyte subpopulations in the hippocampus with distinct molecular signatures. Transcriptomic shifts during aging differ between both subpopulations. Aged ATP1B2+ astrocytes rewire their metabolism while double‐positive aged astrocytes display a reduced synaptogenic capability.
Casares-Crespo L, Cano-Jaimez M, Anta R, Ripollés-Boix A, Robledo-Yagüe F, Zúñiga-Trejos S, Mira H.   +6 more
europepmc   +2 more sources

Proteomic Profiling of Nodal T‐Follicular Helper Cell Lymphoma Reveals Distinct Biological Signatures and Improves Prognostic Stratification Beyond the IPI

American Journal of Hematology, EarlyView.
ABSTRACT Nodal T‐follicular helper cell lymphoma (nTFHL), most commonly of angioimmunoblastic type, exhibits heterogeneous clinical outcomes that are not fully explained by current clinical and biological variables. While genetic alterations have been extensively investigated, its proteomic landscape remains largely unexplored.
David Beauvais, Yanis Zirem, Tristan Cardon, Soulaimane Aboulouard, Romain Dubois, Isabelle Fournier, Franck Morschhauser, Michel Salzet, Ibrahim Yakoub‐Agha, Marie Duhamel   +9 more
wiley   +1 more source

Evaluation of commercially available small RNASeq library preparation kits using low input RNA

BMC Genomics, 2018
Background Evolving interest in comprehensively profiling the full range of small RNAs present in small tissue biopsies and in circulating biofluids, and how the profile differs with disease, has launched small RNA sequencing (RNASeq) into more frequent ...
Ashish Yeri, Amanda Courtright, Kirsty Danielson, Elizabeth Hutchins, Eric Alsop, Elizabeth Carlson, Michael Hsieh, Olivia Ziegler, Avash Das, Ravi V. Shah, Joel Rozowsky, Saumya Das, Kendall Van Keuren-Jensen   +12 more
doaj   +1 more source

Nasal Tuft Cell Markers Are Predictive of Overall Survival in Sinonasal Neuroendocrine and Sinonasal Undifferentiated Carcinomas

International Forum of Allergy &Rhinology, EarlyView.
ABSTRACT Background Sinonasal neuroendocrine carcinoma (SNEC) and sinonasal undifferentiated carcinoma (SNUC) are rare aggressive malignancies with no established prognostic markers. Because features of POU2F3+ small cell lung carcinomas have been identified in both tumors, we evaluated immunostaining for POU2F3, a tuft cell transcription factor, and ...
Nikolina Dioufa, Ricardo V. Bruneto, Jennifer E. Douglas, Nithin D. Adappa, James N. Palmer, Noam A. Cohen, Michael A. Kohanski   +6 more
wiley   +1 more source

A host subtraction database for virus discovery in human cell line sequencing data [version 3; peer review: 2 approved]

F1000Research, 2019
The human cell lines HepG2, HuH-7, and Jurkat are commonly used for amplification of the RNA viruses present in environmental samples. To assist with assays by RNAseq, we sequenced these cell lines and developed a subtraction database that contains ...
Jason R. Miller, Kari A. Dilley, Derek M. Harkins, Timothy B. Stockwell, Reed S. Shabman, Granger G. Sutton   +5 more
doaj   +1 more source