Results 71 to 80 of about 99,185 (278)

Spatiotemporal and quantitative analyses of phosphoinositides – fluorescent probe—and mass spectrometry‐based approaches

FEBS Letters, EarlyView.
Fluorescent probes allow dynamic visualization of phosphoinositides in living cells (left), whereas mass spectrometry provides high‐sensitivity, isomer‐resolved quantitation (right). Their synergistic use captures complementary aspects of lipid signaling. This review illustrates how these approaches reveal the spatiotemporal regulation and quantitative
Hiroaki Kajiho, Shin Morioka, Junko Sasaki, Takehiko Sasaki   +3 more
wiley   +1 more source

Epigenetics decouples mutational from environmental robustness. Did it also facilitate multicellularity? [PDF]

PLoS Computational Biology, 2014
The evolution of ever increasing complex life forms has required innovations at the molecular level in order to overcome existing barriers. For example, evolving processes for cell differentiation, such as epigenetic mechanisms, facilitated the ...
Saurabh Gombar, Thomas MacCarthy, Aviv Bergman   +2 more
doaj   +1 more source

On the possible role of robustness in the evolution of infectious diseases [PDF]

Chaos: An Interdisciplinary Journal of Nonlinear Science, 2010
Robustness describes the capacity for a biological system to remain canalized despite perturbation. Genetic robustness affords maintenance of phenotype despite mutational input, necessarily involving the role of epistasis. Environmental robustness is phenotypic constancy in the face of environmental variation, where epistasis may be uninvolved. Here we
James B. Pease, C. Brandon Ogbunugafor, Paul E. Turner   +2 more
openaire   +3 more sources

A Cre‐dependent lentiviral vector for neuron subtype‐specific expression of large proteins

FEBS Letters, EarlyView.
We designed a versatile and modular lentivector comprising a Cre‐dependent switch and self‐cleaving 2A peptide and tested it for co‐expression of GFP and a 2.8 kb gene of interest (GOI) in mouse cortical parvalbumin (PV+) interneurons and midbrain dopamine (TH+) neurons.
Weixuan Xue, Sandrine Picaud, Régine Hepp, Stéphanie Pons, Uwe Maskos, Bertrand Lambolez, Ludovic Tricoire   +6 more
wiley   +1 more source

On the temporal evolution of backbone topological robustness [PDF]

Proceedings of the 2013 18th European Conference on Network and Optical Communications & 2013 8th Conference on Optical Cabling and Infrastructure (NOC-OC&I), 2013
As society progressively depends on large-scale telecommunications networks and their connectivity rapidly rises over time, it becomes of vital importance to study their topological features. On the backbone level uncertain disturbances of its constituent parts may affect a sizable proportion of the population, thus this can well explain the recent ...
Athanasios Balmpakakis, Dimitris Maniadakis, Dimitris Varoutas   +2 more
openaire   +2 more sources

The IQ‐compete assay for measuring mitochondrial protein import efficiencies in living yeast cells

FEBS Letters, EarlyView.
The efficiency of mitochondrial protein import depends on the properties of the newly synthesized precursor proteins. The Import and de‐Quenching Competition (IQ‐compete) assay is a novel method to monitor the import efficiency of different proteins by fluorescence in living yeast cells.
Yasmin Hoffman, Annika Egeler, Saskia Rödl, Johannes M. Herrmann   +3 more
wiley   +1 more source

Parasites lead to evolution of robustness against gene loss in host signaling networks

Molecular Systems Biology, 2008
Many biological networks can maintain their function against single gene loss. However, the evolutionary mechanisms responsible for such robustness remain unclear.
Marcel Salathé, Orkun S Soyer
doaj   +1 more source

Targeting EZH2 reverses thyroid cell dedifferentiation and enhances iodide uptake in anaplastic thyroid cancer

FEBS Letters, EarlyView.
Anaplastic thyroid cancer (ATC) lacks iodide uptake ability due to MAPK activation increasing the expression of the histone methyltransferase EZH2, which represses thyroid differentiation genes (TDGs) such as the sodium iodide symporter (NIS). Dual inhibition of MAPK (U0126) and EZH2 (EPZ6438/Tazemetostat) reverses this mechanism, thus restoring TDG ...
Diego Claro de Mello, Marcella Maringolo Cristovão, Guilherme Henrique, Vinicius Gonçalves Rodrigues, Caroline Serrano‐Nascimento, Edna Teruko Kimura, Cesar Seigi Fuziwara   +6 more
wiley   +1 more source

Comparing self‐reported race and genetic ancestry for identifying potential differentially methylated sites in endometrial cancer: insights from African ancestry proportions using machine learning models

Molecular Oncology, EarlyView.
Integrating ancestry, differential methylation analysis, and machine learning, we identified robust epigenetic signature genes (ESGs) and Core‐ESGs in Black and White women with endometrial cancer. Core‐ESGs (namely APOBEC1 and PLEKHG5) methylation levels were significantly associated with survival, with tumors from high African ancestry (THA) showing ...
Huma Asif, J. Julie Kim
wiley   +1 more source

Violations of robustness trade‐offs

Molecular Systems Biology, 2010
Biological robustness is a principle that may shed light on system‐level characteristics of biological systems. One intriguing aspect of the concept of biological robustness is the possible existence of intrinsic trade‐offs among robustness, fragility ...
Hiroaki Kitano
doaj   +1 more source