Results 81 to 90 of about 1,811,216 (344)

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

open access: yesMolecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra   +10 more
wiley   +1 more source

HSP70 governs permeability and mechanotransduction in primary human endothelial cells

open access: yesFEBS Open Bio, EarlyView.
HSP70 chemical inhibition reduces endothelial cell proliferation and increases permeability, the latter supported by normal interendothelial junctional protein distribution. HSP70 also plays a role in shear stress response, a hemodynamic force naturally present in blood vessels and correlated with vessel protection.
Andrea Pinto‐Martinez   +5 more
wiley   +1 more source

Upside-Down Rotation of a Breast Implant with Double Capsule Formation after Aesthetic Breast Augmentation: A Case Report [PDF]

open access: yesArchives of Aesthetic Plastic Surgery, 2018
Some complications can occur after augmentation mammoplasty with a prosthesis. Double capsule formation is an uncommon complication, and it is especially rare for it to be accompanied by malrotation of a round implant.
Wang Seok Lee, Sang Gue Kang
doaj   +1 more source

Homologous expression and purification of human HAX‐1 for structural studies

open access: yesFEBS Open Bio, EarlyView.
This research protocol provides detailed instructions for cloning, expressing, and purifying large quantities of the intrinsically disordered human HAX‐1 protein, N‐terminally fused to a cleavable superfolder GFP, from mammalian cells. HAX‐1 is predicted to undergo posttranslational modifications and to interact with membranes, various cellular ...
Mariana Grieben
wiley   +1 more source

ROTATING GLOBULAR CLUSTERS [PDF]

open access: yesThe Astrophysical Journal, 2013
Internal rotation is considered to play a major role in the dynamics of some globular clusters. However, in only few cases it has been studied by quantitative application of realistic and physically justified global models. Here we present a dynamical analysis of the photometry and three-dimensional kinematics of omega Cen, 47 Tuc, and M15, by means of
P. Bianchini   +3 more
openaire   +2 more sources

Day/night variations of myeloid and lymphoid cell subsets in the murine inguinal lymph node

open access: yesFEBS Open Bio, EarlyView.
The circadian system is involved in the temporal regulation of the immune system. Our study reveals that two innate immune populations, NKT cells and neutrophils, predominate at the beginning of the day in healthy mice, highlighting how the time of day influences immune responses.
Paula M. Wagner   +6 more
wiley   +1 more source

Long-Term Tillage and Crop Rotation Regimes Reshape Soil-Borne Oomycete Communities in Soybean, Corn, and Wheat Production Systems [PDF]

open access: gold, 2023
Alison Claire Gahagan   +6 more
openalex   +1 more source

SmallTalk: a novel small‐sized fusion tag for peptide expression and purification

open access: yesFEBS Open Bio, EarlyView.
The SmallTalk fusion tag allows for the efficient expression and purification of soluble recombinant proteins or peptides in Escherichia coli. Testing with SmallTalk‐GFP confirmed that the proteins were soluble and folded correctly, while SmallTalk‐Bin1b maintained its antimicrobial activity against various bacterial isolates. This streamlined workflow
Atika Tariq   +3 more
wiley   +1 more source

Unmanned Aerial Vehicle-Based Techniques for Monitoring and Prevention of Invasive Apple Snails (Pomacea canaliculata) in Rice Paddy Fields

open access: yesAgriculture
The destructive impact of invasive apple snail (Pomacea canaliculata) on young rice seedlings has garnered global attention, particularly in warm regions where rice production occurs.
Senlin Guan   +3 more
doaj   +1 more source

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