Cysteine Methylation Controls Radical Generation in the Cfr Radical AdoMet rRNA Methyltransferase [PDF]
, 2013 The 'radical S-adenosyl-L-methionine (AdoMet)' enzyme Cfr methylates adenosine 2503 of the 23S rRNA in the peptidyltransferase centre (P-site) of the bacterial ribosome.
Driesener, RC +17 more
core +2 more sources
Biphasic kinetics of the human DNA repair protein MED1 (MBD4), a mismatch-specific DNA N-glycosylase
, 2000 The human protein MED1 (also known as MBD4) was previously isolated in a two-hybrid screening using the mismatch repair protein MLH1 as a bait, and shown to have homology to bacterial base excision repair DNA N-glycosylases/lyases.
Genuardi, M, Genuardi, Maurizio
core +1 more source
Rrp47p is an exosome-associated protein required for the 3 ' processing of stable RNAs [PDF]
, 2003 Related exosome complexes of 3'-->5' exonucleases are present in the nucleus and the cytoplasm. Purification of exosome complexes from whole-cell lysates identified a Mg2+-labile factor present in substoichiometric amounts. This protein was identified
Tollervey, D +4 more
core +1 more source
, 2002 The promutagenic and genotoxic exocyclic DNA adduct 1,N2-ethenoguanine (1,N2-εG) is a major product formed in DNA exposed to lipid peroxidation-derived aldehydes in vitro.
Saparbaev, Murat +14 more
core +1 more source
Function of the upstream activating factor in chromatin structure organization and transcriptional regulation at the yeast ribosomal DNA [PDF]
, 2010 Chromatin is the template of all processes involved in DNA metabolism in the eukaryotic cell. Accordingly, chromatin is a dynamic structure which changes in its composition and posttranslational modification correlating with the functional state of a ...
Götze, Hannah
core +1 more source
The kinetics of uracil-N-glycosylase distribution inside replication foci
Mismatched nucleobase uracil is commonly repaired through the base excision repair initiated by DNA uracil glycosylases. The data presented in this study strongly indicate that the nuclear uracil-N-glycosylase activity and nuclear protein content in ...
Ivo Frydrych +4 more
core +1 more source
N-Glycosyl Bond Formation Catalyzed by Human Alkyladenine DNA Glycosylase
, 2016 The removal of damaged bases by DNA glycosylases is thought to be effectively irreversible, because of an overall equilibrium that favors hydrolysis over synthesis of the N-glycosyl bond.
Suzanne J. Admiraal (2257027) +1 more
core +1 more source
DNA Glycosylase Activity Assay Based on Streptavidin Paramagnetic Bead Substrate Capture
, 2001 A method to detect DNA glycosylase activity is described. The substrate used was an oligodeoxyribonucleotide with a unique hypoxanthine base, but has general application to any DNA glycosylase or endonuclease.
O'Connor, Timothy R, Xia, Liqun
core +1 more source
¹⁵N stable isotope probing of pulp and paper wastewaters [PDF]
, 2008 Stable isotope probing (SIP) is an established technique that can be applied to identify the metabolically active micro-organisms within a microbial population.
Addison, Sarah Louise
core
DNA N-glycosylase deficient mice:a tale of redundancy
, 2003 The generation of mouse models of base excision repair deficiency has resulted in a re-examination of the cellular defence mechanisms that exist to counteract oxidative base damage.
Elder, RH +3 more
core +1 more source