In Situ Hybridization and RT-PCR Detection of Nervous Necrosis Virus in Fourfinger Threadfin, Eleutheronema tetradactylum, in Taiwan. [PDF]
ABSTRACT Between April and July 2020, suspected outbreaks of nervous necrosis virus (NNV) infection were observed in fourfinger threadfin (Eleutheronema tetradactylum) fingerling hatcheries in Pingtung County, southern Taiwan. Affected fish exhibited spiral swimming behaviour and abdominal distension, resulting in mortality rates between 50% and 100 ...
Hsieh CY, Wu ST, Lin CY.
europepmc +2 more sources
Genotypic Characterization of <i>Infectious Spleen and Kidney Necrosis Virus</i> (ISKNV) in Southeast Asian Aquaculture. [PDF]
Infectious spleen and kidney necrosis virus (ISKNV) is a species within the genus Megalocytivirus (family Iridoviridae), which causes high mortality disease in many freshwater and marine fish species. ISKNV was first reported in Asia and is an emerging threat to aquaculture with increasing global distribution, in part due to its presence in ornamental ...
Fusianto CK +7 more
europepmc +2 more sources
Risk of Spread of Megalocytivirus pagrus1 (Infectious Spleen and Kidney Necrosis Virus) From Frozen Fillets. [PDF]
ABSTRACT Infectious spleen and kidney necrosis virus (ISKNV) is a significant threat to global aquatic food security by causing large‐scale mortality in the aquaculture of tilapia (Oreochromis niloticus) and mandarin fish (Siniperca chuatsi). ISKNV is a genogroup of Megalocytivirus pagrus1, along with RSIV and TRBIV, and their recent listing as WOAH ...
Becker JA +6 more
europepmc +2 more sources
Development and Validation of a Viability RT-qPCR Assay for Detecting Infectious Spring Viraemia of Carp Virus (SVCV). [PDF]
ABSTRACT Spring viremia of carp virus (SVCV) is a contagious pathogen associated with significant mortality and economic losses in freshwater aquaculture. Although reverse transcription quantitative PCR (RT‐qPCR) enables rapid detection, it does not distinguish between infectious and non‐infectious viral particles, which may lead to overestimation of ...
Son HJ +9 more
europepmc +2 more sources
The red sea bream iridovirus (RSIV) belonging to genus Megalocytivirus is responsible for red sea bream iridoviral disease (RSIVD) in marine and freshwater fishes. Although several diagnostic
Guk Hyun Kim +6 more
doaj +1 more source
Development of a Propidium Monoazide-Based Viability Quantitative PCR Assay for Red Sea Bream Iridovirus Detection. [PDF]
Red sea bream iridovirus (RSIV) is an important aquatic virus that causes high mortality in marine fish. RSIV infection mainly spreads through horizontal transmission via seawater, and its early detection could help prevent disease outbreaks.
Kim KH +5 more
europepmc +2 more sources
Development of a High-Hydrostatic-Pressure-Treated Recombinant Vaccine Targeting the Major Capsid Protein of Red Sea Bream Iridovirus. [PDF]
Red sea bream (Pagrus major) aquaculture represents one of the most economically important marine aquaculture industries in Japan and East Asia. However, viral diseases, particularly those caused by red sea bream iridovirus (RSIV), pose a serious threat ...
Sawasaki Y +15 more
europepmc +2 more sources
Multiplex PCR method for distinguishing two genotypes of Megalocytivirus isolates in Korea
Megalocytivirus is a genus of piscine viruses that belongs to the Iridoviridae family, and this family includes red seabream iridovirus (RSIV), turbot reddish body iridovirus (TRBIV), and infectious spleen and kidney necrosis virus.
Hanchang Sohn +6 more
doaj +1 more source
The analytical and diagnostic performances of methods for detecting red sea bream iridovirus (RSIV), which infects marine fish, have not been evaluated. As disease management and transmission control depend on early and reliable pathogen detection, rapid
Kyung-Ho Kim +8 more
doaj +1 more source
Emergence of Decapod iridescent virus 1 in cultured shrimp from Taiwan in 2020
Maximum likelihood tree constructed in MEGA X, assessed by a neighbour‐joining bootstrap analysis (1000 replications), based on 1123‐bp nucleotide sequences of major capsid protein of DIV1s from Taiwan and reference sequences of the genera, Iridovirus, Chloriridovirus, Lymphocystivirus, Megalocytivirus and Ranavirus from GenBank database (Table 1). The
Lu Yiping +4 more
wiley +1 more source

