Results 51 to 60 of about 608 (160)

RsiV directly binds lysozyme.

, 2014
A. Co-purification experiment showing 6×His-2×Flag-RsiV59–285 and HEW lysozyme elute from a Ni affinity column in the same fractions. A mock lysate from BL21 cells containing an empty vector control plasmid did not show this same ability to pull down HEW
Jon C. Houtman (638203), Craig D. Ellermeier (94495), Jessica L. Hastie (638200), Carolina Sepúlveda (638202), Katrina T. Forest (266995), Kyle B. Williams (638201)   +5 more
core   +1 more source

Megalocytiviruses

Viruses, 2012
The genus Megalocytivirus, represented by red sea bream iridovirus (RSIV), the first identified and one of the best characterized megalocytiviruses, Infectious spleen and kidney necrosis virus (ISKNV), the type species of the genus, and numerous other ...
Kazuhiro Nakajima, Jun Kurita
doaj   +1 more source

Outbreak investigation attributes Infectious spleen and kidney necrosis virus as a necessary cause of a mortality epidemic in farmed grouper (Epinephelus spp.) in Bali, Indonesia

Aquaculture Reports, 2021
In 2016, famers reported mass mortality events in hybrid grouper within 2–4 weeks following transfer to sea cages on the northern coast of Bali. The objective was to obtain an aetiological diagnosis using a broad range of traditional and emerging ...
Cahya Fusianto, Paul M. Hick, Murwantoko, Afri Herlambang, Richard J. Whittington, Joy A. Becker   +5 more
doaj   +1 more source

Extracellular domain of RsiV from C. difficile and E. faecalis RsiV bind lysozyme.

, 2014
Samples from a lysozyme pull-down assay were separated on a 15% SDS-PAGE gel and stained with Coomassie brilliant blue. The pull down assay was performed as described in Materials and Methods. Recombinant 6×His-RsiVC-term from both E.
Jon C. Houtman (638203), Craig D. Ellermeier (94495), Jessica L. Hastie (638200), Carolina Sepúlveda (638202), Katrina T. Forest (266995), Kyle B. Williams (638201)   +5 more
core   +1 more source

Characterization of Red Sea Bream (Pagrus major) Interferon Regulatory Factor 5 and 6 Genes and Their Expression in Response to RSIV Infection

Fishes, 2023
Interferon regulatory factors (IRFs) play crucial roles in antiviral processes, such as in the transcriptional induction of interferon (IFN) and IFN-stimulated genes (ISGs). In this study, the genes encoding IRF5 and IRF6 were identified in Pagrus major,
Kyung-Ho Kim, Min-Soo Joo, Gyoungsik Kang, Won-Sik Woo, Min-Young Sohn, Ha-Jeong Son, Chan-Il Park   +6 more
doaj   +1 more source

RsiV binding to lysozyme is required for site-1 cleavage of RsiV.

, 2016
Site-1 cleavage of RsiV mutants in response to increasing concentration of lysozyme. Overnight B. subtilis strains were grown to OD600 of 1 in LB IPTG. Strains were divided into 1.5 ml aliquots and incubated with increasing concentrations of lysozyme (0,
Jon C. Houtman (638203), Craig D. Ellermeier (94495), Jessica L. Hastie (638200), Lindsey L. Bohr (3108141), Lokesh Gakhar (367312), Kyle B. Williams (638201)   +5 more
core   +1 more source

Site-1 cleavage of RsiV is required for σ^V activation.

, 2014
A. Model showing location of site-1 cleavage RsiV. Cells producing RsiV-6×His, which is under control of the IPTG inducible promoter Phyper-spank (Phs) (Phs-rsiV-6×his ΔsigVrsiV::kan; JLH548) were protoplasted with HEW lysozyme.
Jon C. Houtman (638203), Craig D. Ellermeier (94495), Jessica L. Hastie (638200), Carolina Sepúlveda (638202), Katrina T. Forest (266995), Kyle B. Williams (638201)   +5 more
core   +1 more source

RsiV provides lysozyme resistance in vivo.

, 2016
RsiV provides lysozyme resistance in vivo.
Jon C. Houtman (638203), Craig D. Ellermeier (94495), Jessica L. Hastie (638200), Lindsey L. Bohr (3108141), Lokesh Gakhar (367312), Kyle B. Williams (638201)   +5 more
core   +1 more source

ULTRASTRUCTURAL ANALYSIS OF GRUNT FIN (GF) CELLS TREATED WITH RED SEA BREAM IRIDOVIRUS (RSIV; family Iridoviridae, genus Megalocytivirus) IN COMBINATIONS WITH INTERFERONS AND SPLENIC SUBSTANCES [PDF]

, 2010
The genus Megalocytivirus in the family of Iridoviridae encompasses isolate of red sea bream iridovirus (RSIV). In the present study, grunt fin (GF) cells were treated with red sea bream iridovirus (RSIV) in combinations with interferons (IFNs) and ...
Mahardika, Ketut, Miyazaki, Teruo
core   +1 more source

Data on molecular characterization and expression profiles of the NF-κB repressing factor gene in red sea bream (Pagrus major)

Data in Brief, 2019
Nuclear factor-kappaB (NF-κB) repressing factor (NKRF) specifically inhibits the transcriptional activity of NF-κB protein. The PmNKRF cDNA is composed of 757 amino acid residues. Alignment analysis revealed that the G-patch and R3H domains are conserved
Kwang-Min Choi, Jee Youn Hwang, Mun-Gyeong Kwon, Ji-Min Jeong, Jung Soo Seo, Seong Don Hwang, Bo-Yeong Jee, Mu-Chan Kim, Chan-Il Park   +8 more
doaj   +1 more source