Results 91 to 100 of about 565,475 (306)

Development of multiplexed reverse-transcription loop-mediated isothermal amplification for detection of SARS-CoV-2 and influenza viral RNA

open access: yesBioTechniques, 2021
The ongoing pandemic has demonstrated the utility of widespread surveillance and diagnostic detection of the novel SARS-CoV-2. Reverse-transcription loop-mediated isothermal amplification (RT-LAMP) has enabled broader testing, but current LAMP tests only 
Yinhua Zhang, Nathan A Tanner
doaj   +1 more source

Direct comparison of SARS-CoV-2 detection using RT-qPCR and extraction-free RT-LAMP.

open access: yes, 2022
Direct comparison of SARS-CoV-2 detection using RT-qPCR and extraction-free RT-LAMP.
Prem K. Premsrirut (12646751)   +15 more
core   +1 more source

Vitamin C‐Induced Photo‐Redox Threshold Enables High‐Fidelity Volumetric Printing of Collagen

open access: yesAdvanced Materials, EarlyView.
Tomographic volumetric printing (TVP) of native proteins is limited by uncontrolled background crosslinking. Here, vitamin C is introduced as a biocompatible redox regulator to establish a tunable nonlinear polymerization threshold response for TVP.
Bin Wang   +5 more
wiley   +1 more source

Visual detection of the human metapneumovirus using reverse transcription loop-mediated isothermal amplification with hydroxynaphthol blue dye

open access: yesVirology Journal, 2012
Background Human metapneumovirus (hMPV) is a major cause of acute respiratory infections ranging from wheezing to bronchiolitis and pneumonia in children worldwide.
Wang Xiang   +7 more
doaj   +1 more source

Self‐Sealing Microneedles Improve Precision and Reduce Reflux in Subretinal Cell Transplantation

open access: yesAdvanced Materials Technologies, EarlyView.
Ultrafine 49G microneedles enable subretinal delivery of induced pluripotent stem cell‐derived retinal pigment epithelial cells through a 50 µm outer diameter tip. Compared with conventional 38G microcannulas, these microneedles reduce cell suspension reflux in a rabbit model, highlighting the potential of microinvasive delivery instruments to improve ...
Jared Ching   +9 more
wiley   +1 more source

Detection of SARS-CoV-2 RNA in Urine by RT-LAMP: A Very Rare Finding

open access: yes, 2021
Detection of SARS-CoV-2 is routinely performed in naso/oropharyngeal swabs samples from patients via RT-qPCR. The RT-LAMP technology has also been used for viral RNA detection in respiratory specimens with both high sensitivity and specificity. Recently,
Juan García-Bernalt Diego   +9 more
core   +1 more source

Mitochondria and Neuromast Tagging With Fluorescent Gallium‐Triapine Analogues: In Cellulo MP FLIM and Zebrafish Live Imaging

open access: yesAdvanced Science, EarlyView.
Fluorescent BODIPY‐conjugated thiosemicarbazone ligands and their Ga(III), In(III), and Fe(III) complexes, inspired by Triapine, are developed as theranostic agents. Multiphoton FLIM and confocal microscopy in cancer cells and zebrafish reveal real‐time uptake, mitochondrial localisation, and whilst spectroscopic assays indicated preserved complex ...
Megan J. Green   +15 more
wiley   +1 more source

RT-LAMP for BYV detection v1

open access: yes
This protocol describes the process of detecting beet yellows virus (BYV) in plant material utilising a quick and easy colorimetric assay. The starting point is total RNA extracted from the material. The positive results are shown in yellow color, while negative samples stay bright pink - an example of these results is provided at the end of the ...
Marta Niedzicka, Stephen Byrne
openaire   +1 more source

Atomic Tuning of Metal‐Support Interactions for Pathway‐Selective CO2 Photoreduction on TiO2

open access: yesAdvanced Science, EarlyView.
Single‐atom Fe and Cu catalysts anchored on TiO2 steer photocatalytic CO2 reduction toward distinct pathways. Fe sites favor rapid *CO desorption and selective CO formation, whereas Cu sites stabilize *CHO intermediates, enabling deep reduction and C─C coupling. Combined spectroscopy and DFT calculations reveal how metal–support interactions and oxygen
Dongyun Kim   +13 more
wiley   +1 more source

GHRHR Deficiency Enhances Retinal Ganglion Cell Survival and Visual Functions in Experimental Glaucoma by Inhibiting Ferroptosis

open access: yesAdvanced Science, EarlyView.
Glaucoma, a major cause of blindness, involves retinal ganglion cell (RGC) degeneration. This study shows growth hormone‐releasing hormone receptor (GHRHR) deficiency preserves RGC survival and restores vision, unlike activation which only aids survival.
Yan Tong   +24 more
wiley   +1 more source

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