Results 111 to 120 of about 565,475 (306)

Covalently Linked Peptides and Membrane Potential Enable CyaA Segment Translocation

Advanced Science, EarlyView.
Using a newly developed Droplet Interface Bilayer‐Pipette assay, the authors show that two segments of the CyaA toxin cross membranes by distinct mechanisms. One segment translocates spontaneously, whereas the translocation of the other depends on membrane potential.
Gaia Scilironi, Nicolas Carvalho, Jacinthe Frangieh, Corentin Léger, Dorothée Raoux‐Barbot, J. Iñaki Guijarro, Daniel Ladant, Sophie Cribier, Nicolas Rodriguez, Alexandre Chenal   +9 more
wiley   +1 more source

Development and validation of direct RT-LAMP for SARS-CoV-2 [PDF]

, 2020
Abstract We have developed a reverse-transcriptase loop mediated amplification (RT-LAMP) method targeting genes encoding the Spike (S) protein and RNA-dependent RNA polymerase (RdRP) of SARS-CoV-2. The LAMP assay achieves comparable limit of detection as commonly used RT-PCR protocols based on artificial targets, recombinant Sindbis ...
Mohon, Abu Naser, Hundt, Jana, van Marle, Guido, Pabbaraju, Kanti, Berenger, Byron, Griener, Thomas, Lisboa, Luiz, Church, Deirdre, Czub, Markus, Greninger, Alexander, Jerome, Keith, Doolan, Cody, Pillai, Dylan R   +12 more
openaire   +1 more source

Validation and optimization of the loop-mediated isothermal amplification (LAMP) technique for rapid detection of wheat stripe mosaic virus, a wheat-infecting pathogen

Journal of Genetic Engineering and Biotechnology
Background: Wheat stripe mosaic virus (WhSMV) is a significant wheat pathogen that causes substantial yield losses in Brazil and other countries. Although several detection methods are available, reliable and efficient tools for on-site WhSMV detection ...
Anderson Varela de Andrade, Fernando Sartori Pereira, Fabio Nascimento da Silva, Gustavo Felippe da Silva, Maria de Lourdes Borba Magalhães   +4 more
doaj   +1 more source

The multiplex SFTSV/OT/IC RT-LAMP primer sets.

, 2022
The multiplex SFTSV/OT/IC RT-LAMP primer sets.
Chae Seung Lim (1888042), Woong Sik Jang (9367435), Jeonghun Nam (813361), Dae Won Park (10681659), Young Lan Choe (12096064), Da Hye Lim (12096061), Kyung Chul Moon (7887080), Chaewon Kim (5522381), Minkyeong Choi (12096067), Insu Park (9257728)   +9 more
core   +1 more source

Photobiomodulation‑Engineered Extracellular Vesicles Enhance Neural Differentiation via UFL1‑Mediated UFMylation in Spinal Cord Injury

Advanced Science, EarlyView.
Photobiomodulated microglia release engineered extracellular vesicles that deliver UFL1 to the injured spinal cord. UFL1 competitively binds p53 with MDM2, inhibiting p53 ubiquitination and stabilizing p53 signaling. This dual mechanism simultaneously promotes anti‐inflammatory microglial polarization and directs neural stem cell differentiation toward
Yunxiao Fang, Zuomeng Wu, Ruocheng Guo, Cancan Wang, Yue Qin, Yixiang Dong, Aoyun Li, Ao Liu, Chongyu Jia, Tianyu Han, Peiwen Song, Lei Chen, Cailiang Shen   +12 more
wiley   +1 more source

Noroviruses Surrogate Detection Using Loop-Mediated Isothermal Amplification (LAMP), Conventional RT-PCR and Quantitative RT-PCR in Sg. Tekala and Sg. Gabai Stream Water

MATEC Web of Conferences, 2017
A preliminary study was carried out in order to evaluate a reverse transcriptase loop-mediated isothermal amplification (RT-LAMP) assay for detecting Noroviruses (NoV) RNA surrogates as external standard for NoV.The detection limit of the NoV RT-LAMP ...
Khairuddin Dzulaikha, Mohd Yusof Nurul Yuziana, Jani Jan Maizatulriah, Ab. Wahid Marfiah   +3 more
doaj   +1 more source

Primers designed for the RT-LAMP.

, 2018
Primers designed for the RT-LAMP.
Oumar Faye (32488), Michaël Bekaert (121005), Stefanie Wehner (615655), Mohammed Bakheit (4960669), Manfred Weidmann (448254), Benjamin Lopez-Jimena (4854436), Graham Harold (73273), Sieghard Frischmann (710564), Amadou Alpha Sall (169451)   +8 more
core   +1 more source

Primer sets that were optimized for RT-LAMP.

, 2022
Primer sets that were optimized for RT-LAMP.
Camila González (254578), David Duplat (12530750), Patricio Ponce (7221929), Joel D. Pearson (10813079), Marcela Guevara-Suarez (12530768), Rod Bremner (9239), Tony Mazzulli (138922), Keith Pardee (603874), Jim Woodgett (10813097), Adriana Bernal (208832), Katariina Jaenes (12530762), Lucas Robinson (8631705), Silvia Restrepo (204944), Philippos Peidis (10813091), Yuxiu Guo (669665), Eliana Veloz-Villavicencio (12530756), Suying Lu (4369963), Stephany D. Villota (11453611), John M. González (7006130), Cielo León (3128919), Seray Cicek (12530765), Paula Benitez-Bolivar (12530753), Valentina Arévalo (12530759), Varsovia E. Cevallos (12530771)   +23 more
core   +1 more source

Mesoporous PU/PEDOT:PSS Electrodes Reveal Population‐Level Stochastic Bioelectrical Dynamics in Marine Diatoms

Advanced Science, EarlyView.
Mesoporous mixed ionic‐electronic PU/PEDOT: PSS electrodes enable label‐free detection of stochastic extracellular bioelectrical dynamics in living Phaeodactylum tricornutum. Living diatoms actively modulate interfacial ionic dynamics, establishing stochastic electrochemical noise as a sensitive probe of microbial bioelectrochemical activity and a new ...
David M. S. Silva, Felipe L. Bacellar, Raquel Amaral, Louis‐Josselin Lavier‐Aydat, Luísa Cortes, Kamal Asadi, Leïla Tirichine, Paulo R. F. Rocha   +7 more
wiley   +1 more source

Specificity of the RT-LAMP assay.

, 2018
Specificity of the RT-LAMP assay.
Oumar Faye (32488), Michaël Bekaert (121005), Stefanie Wehner (615655), Mohammed Bakheit (4960669), Manfred Weidmann (448254), Benjamin Lopez-Jimena (4854436), Graham Harold (73273), Sieghard Frischmann (710564), Amadou Alpha Sall (169451)   +8 more
core   +1 more source