Results 181 to 190 of about 875,650 (289)

Advancing Broad Bean True Mosaic Virus Detection Using Rt-Pcr and Real-Time Rt-Pcr with Novel Primer Set Design

Fumino Nito, Hitoshi Oya, Takayuki Matsuura, Hironobu Yanagisawa   +3 more
openalex   +1 more source

Absolute estimation of initial concentrations of amplicon in a real-time RT-PCR process [PDF]

, 2007
Marjo V. Smith, Chris R. Miller, Michael C. Kohn, Nigel J. Walker, Christopher J. Portier   +4 more
openalex   +1 more source

Spherical Skin Model: Stratified Co‐Culture of Fibroblasts and Keratinocytes on Spherical Beads Toward Compound Screening

Advanced Healthcare Materials, EarlyView.
Models of the human skin must combine the relevant biological contents and suitable biomaterials with the correct spatial organization. Performing compound screening on such in vitro models also requires fast and reproducible production methods of the models.
Elisa Lenzi, Nadia Vertti‐Quintero, Julien Husson, Anne‐Laure Bulteau, Carine Nizard, Karl Pays, Sébastien Sart, Charles N. Baroud   +7 more
wiley   +1 more source

Modification and Validation of a Reference Real-Time RT-PCR Method for the Detection of a New African Horse Sickness Virus Variant. [PDF]

Microorganisms
Morales J, Ruano MJ, Tena-Tomás C, van Schalkwyk A, Loundras EA, Valero-Lorenzo M, López-Herranz A, Romito M, Batten C, Villalba R, Agüero M.   +10 more
europepmc   +1 more source

Development of a Synthetic 3D Platform for Compartmentalized Kidney In Vitro Disease Modeling

Advanced Healthcare Materials, EarlyView.
A fully synthetic, compartmentalized 3D kidney disease model is introduced. The kidney model combines a PEG‐based hydrogel matrix with anisotropic, enzymatically degradable rod‐shaped microgels to spatially arrange a triple co‐culture of key renal epithelial, endothelial, and fibroblast cells.
Ninon Möhl, Daphne Bouwens, Johanna Abele, Aline Hans, Tanja Topic, Daniel Günther, Jitske Jansen, Rafael Kramann, Laura De Laporte   +8 more
wiley   +1 more source

Bioengineered Lymphatic Vessels in Synthetic Matrices to Study Breast Cancer Cell Functions

Advanced Healthcare Materials, EarlyView.
Lymphatic vessels are involved in cancer metastasis. To study the interplay between metastasizing cancer cells and lymphatic vessels under highly reproducible conditions, advanced in vitro models are required. In this work, 3D lymphatic networks are formed in biomimetic hydrogels and their interactions with invasive and non‐invasive cancer cell‐lines ...
Rodi Odabasi, Lisa A. Krattiger, Magdalini Kanari, Mira A. Jacobs, Lukas O. Moser, Mark W. Tibbitt, Cornelia Halin, Martin Ehrbar   +7 more
wiley   +1 more source

New Approach to mRNA Quantification: Additive RT-PCR

BioTechniques, 1996
A. Reyes-Engel, J. García-Villanova, J.L. Dieguez-Lucena, N. Fernández-Arcás, M. Ruiz-Galdón   +4 more
doaj   +1 more source

Development of a TaqMan-Based Duplex Real-Time Quantitative RT-PCR for Detection and Differentiation of Muscovy Duck Reovirus and Novel Duck Reovirus. [PDF]

Pathogens
Liu L, Fu J, Lin M, Wang A, Wu S, Liu C.
europepmc   +1 more source

Plasma‐Polymerized Nanoparticles Presenting Fibrillin‐1 Drive Rapid Re‐Endothelialization of Vascular Grafts

Advanced Healthcare Materials, EarlyView.
Commercial vascular grafts are made from ePTFE, a highly hydrophobic, foreign material that fails at a high rate in small‐diameter applications. Plasma polymer nanoparticles (PPN) are a versatile material functionalisation tool, used here to present fibrillin‐1 fragment PF8 on the graft surface.
Bob S. L. Lee, Yuen Ting Lam, Alex H. P. Chan, Praveesuda L. Michael, Timothy C. Mitchell, Jui Chien Hung, Miguel Santos, Khoon S. Lim, Richard P. Tan, Steven G. Wise   +9 more
wiley   +1 more source

Importance of a heat snap in RT-PCR quantification of rotavirus double-stranded RNA in wastewater.

Environ Sci (Camb)
Kang S, Wettlauffer A, de Korne-Elenbaas J, Niwagaba CB, Strande L, Duong D, Shelden B, Julian TR, Boehm AB.   +8 more
europepmc   +1 more source