Results 201 to 210 of about 890,017 (258)
Prolonged SARS-CoV-2 RNA detection by RT-PCR: variability in cycle threshold values over time. [PDF]
Microbiol SpectrMuñoz-Gallego I +4 more
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Development of multiplex RT-PCR assays containing an internal amplification control for the detection of dicistro-, iflaviruses and CBPV in honey bees. Part 1 - assays design and optimization. [PDF]
Vet Res CommunZdańska D, Rzeżutka A, Pohorecka K.
europepmc +1 more source
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
openaire +2 more sources
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
openaire +2 more sources
Current Protocols in Cell Biology, 2001
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
C P, Paweletz +2 more
openaire +2 more sources
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
C P, Paweletz +2 more
openaire +2 more sources
Detection of Viroids by RT-PCR
2021Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels.
Nerida J, Donovan +2 more
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Journal of Virological Methods, 2000
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
D S, Konet +3 more
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Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
D S, Konet +3 more
openaire +2 more sources
2003
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
openaire +3 more sources
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
openaire +3 more sources
2010
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Kelvin, Li, Anushka, Brownley
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Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Kelvin, Li, Anushka, Brownley
openaire +2 more sources