Results 211 to 220 of about 1,155,913 (246)
Utilization of RT-PCR and Optical Genome Mapping in Acute Promyelocytic Leukemia with Cryptic <i>PML::RARA</i> Rearrangement: A Case Discussion and Systemic Literature Review. [PDF]
Genes (Basel)George GV +5 more
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Correlation of COVID-19 vaccination and RT-PCR ct value among cases in Addis Ababa, Ethiopia: implication for future preparedness. [PDF]
BMC Infect DisAga AM +11 more
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Use of a diagnostic Puumala virus real-time RT-PCR in an orthohantavirus endemic region in the Netherlands. [PDF]
Microbiol SpectrGeeraedts F +9 more
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ƩS COVID-19 is a rapid high throughput and sensitive one-step quadruplex real-time RT-PCR assay. [PDF]
Sci RepKowitdamrong E +4 more
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Detection of Viroids by RT-PCR [PDF]
, 2021 Reverse transcription-polymerase chain reaction (RT-PCR) is an effective method for detecting the presence of viroids in plant tissue. Viroid RNA is converted to cDNA and amplified to detectable levels, making it a fast and useful detection tool, even when the viroid is present at low levels.
Mengji Cao +2 more
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco Faggioli, Marta Luigi
openaire +3 more sources
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco Faggioli, Marta Luigi
openaire +3 more sources
Current Protocols in Cell Biology, 2001
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
Lu Charboneau +2 more
openaire +3 more sources
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
Lu Charboneau +2 more
openaire +3 more sources
Journal of Virological Methods, 2000
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
Fred Brown +3 more
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Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
Fred Brown +3 more
openaire +3 more sources
2010
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Anushka Brownley, Kelvin Li
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Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Anushka Brownley, Kelvin Li
openaire +3 more sources