Results 251 to 260 of about 1,247,639 (287)
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2021
Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
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Amplification of different nucleic acid targets in the same reaction (multiplex polymerase chain reaction) is challenging but an extremely useful tool especially for viroid diagnosis. In the amplification mixtures, several pairs of primers work together in the same conditions to detect different targets.
Francesco, Faggioli, Marta, Luigi
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Current Protocols in Cell Biology, 2001
AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
C P, Paweletz +2 more
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AbstractThis unit presents a collection of protocols for the microisolation, manipulation, and amplification of the RNA content of microdissected cells. Even though emphasis in these protocols is given for microdissected cells, these protocols have successfully been used for bulk tissue (i.e., less than 10 ug).
C P, Paweletz +2 more
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2003
Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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Although reverse transcriptase polymerase chain reaction (RT-PCR) is an extremely sensitive method of mRNA analysis, obtaining quantitative information with this technique can be difficult. This is caused primarily by the fact that there are two sequential enzymatic steps involved: the synthesis of DNA from the RNA template and PCR.
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Automated high throughput RT‐PCR
Laboratory Robotics and Automation, 1996AMGEN's genome project is designed to discover and assess genes that may be of therapeutic value. As an early screen, novel genes of potential importance are investigated by analysis of their in vivo expression pattern using reverse transcriptase-polymerase chain reaction (RT-PCR).
Jason W. Armstrong +4 more
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2010
Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Kelvin, Li, Anushka, Brownley
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Primer design is a crucial initial step in any experiment utilizing PCR to target and amplify a known nucleotide sequence of interest. Properly designed primers will increase PCR amplification efficiency as well as isolate the targeted sequence of interest with higher specificity. Many factors that may limit the success of a primer pair can be detected
Kelvin, Li, Anushka, Brownley
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2011
Reverse transcription PCR (RT-PCR) is one of the most important techniques for analyzing RNA abundance. MicroRNAs (miRNAs) are a group of 20- to 24-nucleotide regulatory small RNAs which play an important role in plants and animals. However, the small size of miRNAs makes them difficult to be detected and quantified by conventional RT-PCR techniques ...
Rui, Shi +3 more
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Reverse transcription PCR (RT-PCR) is one of the most important techniques for analyzing RNA abundance. MicroRNAs (miRNAs) are a group of 20- to 24-nucleotide regulatory small RNAs which play an important role in plants and animals. However, the small size of miRNAs makes them difficult to be detected and quantified by conventional RT-PCR techniques ...
Rui, Shi +3 more
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Real-Time RT-PCR: cDNA Synthesis
Cold Spring Harbor Protocols, 2006INTRODUCTIONThis protocol uses the Superscript II First-Strand Synthesis System for the generation of cDNA from total RNA.
Wolfgang, Kusser +2 more
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Journal of Virological Methods, 2000
Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
D S, Konet +3 more
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Amplification by RT-PCR of the RNA present in foot-and-mouth disease virus particles is inhibited by substances present in the sera of several species. This inhibition appears to be caused by a direct interaction of the substances with the RNA and not the enzymes used for its amplification.
D S, Konet +3 more
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2002
Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Part I. Introduction RT-PCR in Biomedicine: Opportunities Arising from the New Accessibility of mRNA Joe O'Connell The Basics of RT-PCR: Some Practical Considerations Joe O'Connell Part II. Highly Sensitive Detection and Analysis of mRNA Description of Quantitative Competitive RT-PCR Technique to Analyze Minute Amounts of Different mRNAs in Small ...
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Volumetric, Microfluidic Plasmonic RT‐PCR
Small MethodsAbstractDecentralized molecular detection of pathogens remains an important goal for public health. Although polymerase chain reaction (PCR) remains the gold‐standard molecular detection method, thermocycling using Peltier heaters presents challenges in decentralized settings. Recent work has demonstrated plasmonic PCR, where nanomaterials on a surface
Harshit Harpaldas Chellani +15 more
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