Results 81 to 90 of about 2,171,089 (338)

Comparative evaluation of conventional RT-PCR and real-time RT-PCR (RRT-PCR) for detection of avian metapneumovirus subtype A Comparação entre as técnicas de RT-PCR convencional e RT-PCR em tempo real para a detecção do metapneumovírus aviários subtipo A

Ciência Rural, 2009
Avian metapneumovirus (AMPV) belongs to Metapneumovirus genus of Paramyxoviridae family. Virus isolation, serology, and detection of genomic RNA are used as diagnostic methods for AMPV.
Helena Lage Ferreira, Fernando Rosado Spilki, Márcia Mercês Aparecida Bianchi dos Santos, Renata Servan de Almeida, Clarice Weis Arns   +4 more
doaj  

Incidence of Viral Infection of Main Garlic Growing Areas of Gangwon Province in Korea

Research in Plant Disease, 2013
This research was conducted to investigate the incidence of viral infection in Samcheok-shi and Jeongseongun, main garlic growing areas of Gangwon province in Korea.
Ji-Eun Shin, Yeong-Gyu Lee, Byung-Sup Kim   +2 more
doaj   +1 more source

Aberrant expression of nuclear prothymosin α contributes to epithelial‐mesenchymal transition in lung cancer

Molecular Oncology, EarlyView.
Nuclear prothymosin α inhibits epithelial‐mesenchymal transition (EMT) in lung cancer by increasing Smad7 acetylation and competing with Smad2 for binding to SNAI1, TWIST1, and ZEB1 promoters. In early‐stage cancer, ProT suppresses TGF‐β‐induced EMT, while its loss in the nucleus in late‐stage cancer leads to enhanced EMT and poor prognosis.
Liyun Chen, Chung‐Teng Wang, Jia‐Ming Chang, Ai‐Li Shiau, Gia‐Shing Shieh, Yau‐Lin Tseng, Yi‐Ting Yen, Tang‐Hsiu Huang, Li‐Hsin Cheng, Yu‐Chih Wu, Chao‐Liang Wu, Bing‐Hua Su, Pensee Wu   +12 more
wiley   +1 more source

The Plant-Growth-Promoting Rhizobacterium Paenibacillus polymyxa Induces Changes in Arabidopsis thaliana Gene Expression: A Possible Connection Between Biotic and Abiotic Stress Responses

Molecular Plant-Microbe Interactions, 1999
This paper addresses changes in plant gene expression induced by inoculation with plant-growth-promoting rhizobacteria (PGPR). A gnotobiotic system was established with Arabidopsis thaliana as model plant, and isolates of Paenibacillus polymyxa as PGPR ...
Salme Timmusk, E. Gerhart H. Wagner
doaj   +1 more source

Application and optimization of RT-PCR in diagnosis of SARS-CoV-2 infection

medRxiv, 2020
Background: Coronavirus Disease 2019 (COVID-19) caused by Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) has become a global threat to public health.
Guanmin Jiang, X. Ren, Yan Liu, Hongtao Chen, Wei Liu, Zhaowang Guo, Yaqin Zhang, Chaoqun Chen, Jianhui Zhou, Q. Xiao, H. Shan   +10 more
semanticscholar   +1 more source

Determination of ADP/ATP translocase isoform ratios in malignancy and cellular senescence

Molecular Oncology, EarlyView.
The individual functions of three isoforms exchanging ADP and ATP (ADP/ATP translocases; ANTs) on the mitochondrial membrane remain unclear. We developed a method for quantitatively differentiating highly similar human ANT1, ANT2, and ANT3 using parallel reaction monitoring. This method allowed us to assess changes in translocase levels during cellular
Zuzana Liblova, Dominika Maurencova, Barbora Salovska, Marek Kratky, Tomas Mracek, Zuzana Korandova, Alena Pecinova, Pavla Vasicova, David Rysanek, Ladislav Andera, Ivo Fabrik, Rudolf Kupcik, Pavel Kashmel, Pinky Sultana, Vojtech Tambor, Jiri Bartek, Josef Novak, Marie Vajrychova, Zdenek Hodny   +18 more
wiley   +1 more source

RT-PCR Detection of HIV in Republic of Macedonia

Biomolecules & Biomedicine, 2008
The aim of the study was to detect HIV RNA in seropositive patients using RT-PCR method and thus, to establish PCR methodology in the routine laboratory works.
Golubinka Bosevska, Nikola Panovski, Eleni Dokić, Violeta Grunevska   +3 more
doaj   +1 more source

Real-life clinical sensitivity of SARS-CoV-2 RT-PCR test in symptomatic patients

medRxiv, 2020
Importance: Understanding the false negative rates of SARS-CoV-2 RT-PCR testing is pivotal for the management of the COVID-19 pandemic and it has practical implications for patient management in healthcare facilities.
E. Kortela, V. Kirjavainen, M. Ahava, S. Jokiranta, A. But, A. Lindahl, A. Jääskeläinen, A. Jääskeläinen, A. Järvinen, P. Jokela, H. Kallio-kokko, R. Loginov, L. Mannonen, E. Ruotsalainen, T. Sironen, O. Vapalahti, M. Lappalainen, Hanna-Riikka Kreivi, H. Jarva, S. Kurkela, E. Kekäläinen   +20 more
semanticscholar   +1 more source

Q-RT-PCR: data analysis software for measurement of gene expression by competitive RT-PCR [PDF]

Bioinformatics, 1997
We have developed software to assist in the computation of gene expression from data obtained in competitive reverse transcription-polymerase chain reaction (RT-PCR). This report describes the mathematical basis of competitive RT-PCR and discusses the criteria which must be met to permit accurate estimations of gene expression to be obtained using this
Jon K. Hays, Amanda Hayward-Lester, Peter A. Doris   +2 more
openaire   +3 more sources

Loss of proton‐sensing GPR4 reduces tumor progression in mouse models of colon cancer

Molecular Oncology, EarlyView.
G protein‐coupled receptor 4 (GPR4) is a pH‐sensing receptor activated by acidic pH. GPR4 expression is increased in patients with inflammatory bowel disease who are at high risk of developing colorectal cancer. In mouse models, loss of GPR4 attenuated tumor progression. This correlated with increased IL2 and natural killer cell activity.
Leonie Perren, Moana Busch, Pedro A. Ruiz, Ermanno Malagola, Valeria Baumeler, Federica Foti, Adelina Gross, Tobias Grütter, Hendrik Edel, Cordelia Schuler, Kristina Handler, Glenn De Lange, Isabelle C. Arnold, Cheryl de Vallière, Klaus Seuwen, Martin Hausmann, Gerhard Rogler   +16 more
wiley   +1 more source