Results 221 to 230 of about 25,478 (242)

Accumulation of S-Adenosylmethionine by Molds

Agricultural and Biological Chemistry, 1974
Aspergillus tamani accumulated about 20 μmoles of S-adenosylmethionine (SAM) in 1 g of dry cells when cultured secondarily in a medium containing more than 10 mm of l- methionine. The accumulation was not so high when l-methionine was replaced by d- methionine. Addition of nucleic acid-related substances was not effective for the accumulation. Addition
Hiroshi Yoshino, Hitoshi Kusakabe, Akira Kuninaka   +2 more
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Streamlined recycling of S-adenosylmethionine [PDF]

Nature Catalysis, 2019
S-adenosylmethionine (SAM)-dependent methyltransferase enzymes have significant synthetic potential, but their utility as biocatalysts has been limited by the availability of SAM. An elegant and simple method addressing this long-standing problem has now been developed using a halide methyltransferase (HMT) enzyme for SAM regeneration in vitro.
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ChemInform Abstract: POTENTIAL INHIBITORS OF S‐ADENOSYLMETHIONINE‐DEPENDENT METHYLTRANSFERASES. 6. TRUCTURAL MODIFICATIONS OF S‐ADENOSYLMETHIONINE

Chemischer Informationsdienst, 1976
AbstractZur pharmakologischen Untersuchung werden verschiedene modifizierte S‐Adenosylmethionine nach üblichen Verfahren hergestellt.
Yih Shiong Wu, R. T. Borchardt, A. F. Wycpalek, J. A. Huber   +3 more
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Assay of S-adenosylmethionine in human blood

Clinica Chimica Acta, 1971
Abstract Blood plasma levels of S -adenosylmethionine have been measured in humans by a recently developed isotope dilution technique. The procedure involves isolation of the sulfonium compound by ion-exchange chromatography after its extraction with perchloric acid. Data are presented on the blood plasma content of S -adenosylmethionine in humans
ILLIANO G, UTILI, Riccardo, CICALA V.
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S-adenosylmethionine: Stability and stabilization

Bioorganic Chemistry, 1987
Abstract With a developed HPLC technique for the separation of both (+)- and (−)- S -adenosylmethionine (AdoMet) and 1 H NMR analysis of the epimeric S-CH 3 chemical shifts, a kinetic study on the stability of (−)-AdoMet in solution to decomposition and epimerization is described.
Jose R. Matos, Chi-Huey Wong
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Stabilization of S-adenosylmethionine decarboxylase by aminoguanidine

Biochemical Pharmacology, 1993
Aminoguanidine, an inhibitor of copper-containing amine oxidases, is often used in cell culture studies to prevent the oxidation of added polyamines or their derivatives by the amine oxidase present in bovine serum. However, in the present study, we demonstrate that aminoguanidine, besides affecting the degradation of polyamines, may affect one of the ...
Louise Stjernborg, Lo Persson
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Quantitation of S-adenosylmethionine decarboxylase protein

Biochemistry, 1985
A method for the specific labeling of the active site of S-adenosylmethionine decarboxylase was developed. The method consisted of incubating cell extracts with 3H-decarboxylated S-adenosylmethionine and sodium cyanoborohydride in the presence of a spermidine synthase inhibitor.
Akira Shirahata, Kathy L. Christman, Anthony E. Pegg   +2 more
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Chemical methylation of phosphatidylethanolamine by S-adenosylmethionine

Biochemical and Biophysical Research Communications, 1985
The chemical methylation of phosphatidylethanolamine (PE) by S-adenosyl methionine (SAM) is most active when carried out at alkaline pH's. Phosphatidylmonomethylethanolamine (PMME) and phosphatidyldimethylethanolamine are less effective reactants. The PE present in the microsomal and myelin membrane can serve as an acceptor in this reaction. Thin layer
Julian N. Kanfer, Vinay S. Bansal
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[A new method for determination of adenosylmethionine, adenosyl-homocysteine and decarboxylated adenosylmethionine].

Bollettino della Societa italiana di biologia sperimentale, 1980
A rapid, sensitive and specific high performance liquid chromatographic method for the simultaneous separation of natural adenosyl sulfur compounds has been developed. The method can be applied to biological samples for the estimation of S-adenosyl-L-methionine, S-adenosyl-L-homocysteine and S-adenosyl-(5')-3-methylthiopropylamine.
DELLA RAGIONE, Fulvio, PORCELLI, Marina, MANNA, Caterina, ROMEO G, GALLETTI P.   +4 more
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Methionine adenosyltransferase (S-adenosylmethionine synthetase) and S-adenosylmethionine decarboxylase.

Advances in enzymology and related areas of molecular biology, 1984
Herbert Tabor, Celia White Tabor
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