Frontiers in Microbiology, 2015 Quinolone resistance is usually due to mutations in the genes encoding bacterial topoisomerases. However different reports have shown that neither clinical quinolone resistant isolates nor in vitro obtained S.
Alejandra eBernardini +4 more
doaj +1 more source
Molecular evolution of candidate male reproductive genes in the brown algal model Ectocarpus [PDF]
, 2016 Background: Evolutionary studies of genes that mediate recognition between sperm and egg contribute to our understanding of reproductive isolation and speciation. Surface receptors involved in fertilization are targets of sexual selection, reinforcement,
De Clerck, Olivier +2 more
core +6 more sources
Rapid screening of staphylokinase protein variants using an unpurified cell‐free expression system
FEBS Open Bio, EarlyView.An unpurified cell‐free protein synthesis (CFPS) platform enables rapid functional screening of staphylokinase variants. Direct plasminogen‐activation assays performed in microplate format provide real‐time activity readouts, allowing rapid identification and ranking of variants with improved or reduced fibrinolytic activity without protein ...
Maria Tomková +3 more
wiley +1 more source
Molecular and physiological identification of new S-alleles associated with self-(in)compatiblity in local Spanish almond cultivars [PDF]
, 2010 The S-allele characterisation of ‘Alzina’ and ‘Garonde` s’, two local almond cultivars from the island of Majorca, by a multidimensional approach has allowed the confirmation of the presence of the Sf-allele and the identification of a new allele not ...
Alonso Segura, José Manuel +4 more
core
An unfolded protein-induced conformational switch activates mammalian IRE1. [PDF]
, 2017 The unfolded protein response (UPR) adjusts the cell's protein folding capacity in the endoplasmic reticulum (ER) according to need. IRE1 is the most conserved UPR sensor in eukaryotic cells.
Acosta-Alvear, Diego +5 more
core +3 more sources
Mass spectrometry based identification of AMP‐O‐Tris generated by Thermococcus onnurineus Cas10
FEBS Open Bio, EarlyView.Isolated Thermococcus onnurineus Cas10 generates the noncanonical ATP‐derived product AMP‐O‐Tris while in Tris‐containing buffer as identified via mass spectrometry, revealing relaxed nucleophile selectivity under isolated conditions. These findings suggest that multiprotein Csm complex assembly restricts Cas10 reactivity toward canonical cyclic ...
Su‐Jin Lee +6 more
wiley +1 more source
A rapid culture independent methodology to quantitatively detect and identify common human bacterial pathogens associated with contaminated high purity water [PDF]
, 2015 Background: Water and High Purity Water (HPW) distribution systems can be contaminated with human pathogenic microorganisms. This biocontamination may pose a risk to human health as HPW is commonly used in the industrial, pharmaceutical and clinical ...
Barry, Thomas +4 more
core +3 more sources
Advanced Functional Materials, EarlyView.A mechanically tunable hydrogel composed of gelatin, chondroitin sulfate and laminin promotes angiogenesis in vitro without the supplement of growth factors. Endothelial cells morphogenesis was further enhanced by medium conditioned with bioactive glass 58S‐released ions (Ca and Si), thus offering a promising strategy to vascularize 3D tissue ...
Marco Piazzoni +13 more
wiley +1 more source
Other self-compatible sweet cherries varieties from Spain [PDF]
, 2014 Food and Agriculture (FA COST Action FA1104: Sustainable production of high-quality cherries for the European ...
Cachi, Ariana M., Wünsch, Ana
core
Investment in plant research and development bears fruit in China [PDF]
, 2014 Recent rapid progress in plant science and biotechnology in China demonstrates that China’s stronger support for funding in plant research and development (R&D) has borne fruit. Chinese groups have contributed major advances in a range of fields, such as
Kang Chong, Zhihong Xu
core +1 more source