Results 81 to 90 of about 47,037 (210)

Enhanced exonuclease–Cas9 systems promote multiple nucleotide deletions with higher efficiency and broader targeting scope in plants

Journal of Integrative Plant Biology, EarlyView.
Cas9 preferentially generates small indels. By tethering an exonuclease and a DNA‐binding domain (DBD) to Cas9, the Exo‐DBD‐Cas9 system efficiently promotes multiple nucleotide deletions. These expanded deletions enable effective disruption of miRNA‐mediated regulation and relieve translational repression through targeted editing of 3′ untranslated ...
Rui Zhang, Xu Tang, Yao He, Wei Wang, Qiurong Ren, Yiping Qi, Yong Zhang   +6 more
wiley   +1 more source

Streptococcus pyogenes Phospholipase A2 Induces the Expression of Adhesion Molecules on Human Umbilical Vein Endothelial Cells and Aorta of Mice

Frontiers in Cellular and Infection Microbiology, 2017
The Streptococcus pyogenes phospholipase A2 (SlaA) gene is highly conserved in the M3 serotype of group A S. pyogenes, which often involves hypervirulent clones. However, the role of SlaA in S. pyogenes pathogenesis is unclear.
Masataka Oda, Masataka Oda, Hisanori Domon, Hisanori Domon, Mie Kurosawa, Toshihito Isono, Tomoki Maekawa, Tomoki Maekawa, Masaya Yamaguchi, Shigetada Kawabata, Yutaka Terao, Yutaka Terao   +11 more
doaj   +1 more source

Synergistic engineering of Casδ nuclease for robust genome editing

Journal of Integrative Plant Biology, EarlyView.
A hierarchical engineering strategy was used to enhance the editing activity of Casδ, yielding an optimized variant termed enCasδ. This variant enables robust genome editing in animal cells and plants, with overall editing performance comparable to that of SpCas9 and other Cas12 nucleases.
Fanghui Ge, Chenchen Peng, Yang Du, Ying Chen, Zilong Zhao, Meixia Yu, Huairu Feng, Yuyang Xie, Siwei Sun, Shengnan Liu, Beibei Xin, Haiming Zhao, Sen Wu, Chao Bian, Zhijia Yang, Jinsheng Lai, Jian Chen   +16 more
wiley   +1 more source

Necrotizing fasciitis following primary peritonitis caused by Streptococcus pyogenes with covS mutation in a healthy woman: a case report

JA Clinical Reports, 2019
Background Primary peritonitis due to Streptococcus pyogenes (S. pyogenes) is uncommon in patients without comorbid conditions such as immunosuppression, nephritic disease, or liver cirrhosis.
Masashi Inoue, Eisuke Kako, Rie Kinugasa, Fumiaki Sano, Hironobu Iguchi, Kazuya Sobue   +5 more
doaj   +1 more source

A Modified Cas9 Scaffold Allows Extension of the Virus‐Induced Gene Editing Technology to the Large Potyvirus Genus

Plant Biotechnology Journal, EarlyView.
ABSTRACT Plant viruses are recognized as rapid and effective vectors to deliver CRISPR‐Cas reaction components into plants, a strategy termed virus‐induced gene editing (VIGE). However, VIGE is limited by the host range of the viral vectors. Development of new viral vectors to target a broad range of plant species will potentially enable the delivery ...
Fernando Merwaiss, Verónica Aragonés, Arcadio García, José‐Antonio Daròs   +3 more
wiley   +1 more source

THE ROLE OF ARGININE DEIMINASE FROM STREPTOCOCCUS PYOGENES IN INHIBITION MACROPHAGES NITROGEN MONOXIDE (NO) SYNTHESIS

Инфекция и иммунитет, 2018
The protective role of macrophages closely related to the production of bactericidal molecules, in which nitrogen monoxide (NO) play an  important role.
E. A. Starikova, A. V. Sokolov, L. A. Burova, A. S. Golovin, A. M. Lebedeva, V. B. Vasilyev, I. S. Freidlin   +6 more
doaj   +1 more source

CRISPR/Cas‐Mediated Gene Editing in Plant Immunity and Its Potential for the Future Development of Fungal, Oomycete, and Bacterial Pathogen‐Resistant Pulse Crops

Plant, Cell &Environment, Volume 49, Issue 7, Page 3573-3585, July 2026.
ABSTRACT Pulses provide myriad health benefits and are advantageous in an environmental context as a result of their leguminous nature. However, phytopathogenic fungi, oomycetes and bacteria pose a substantial threat to pulse production, at times leading to crop failure.
Stacy D. Singer, Mohammed M. Mukthar, Udaya Subedi, Hari Poudel, Guanqun Chen, Nora Foroud, Syama Chatterton   +6 more
wiley   +1 more source

Superantigenic activity of emm3 Streptococcus pyogenes is abrogated by a conserved, naturally occurring smeZ mutation.

PLoS ONE, 2012
Streptococcus pyogenes M/emm3 strains have been epidemiologically linked with enhanced infection severity and risk of streptococcal toxic shock syndrome (STSS), a syndrome triggered by superantigenic stimulation of T cells. Comparison of S.
Claire E Turner, Mary Sommerlad, Karen McGregor, Frances J Davies, Bruno Pichon, Deborah L W Chong, Leili Farzaneh, Matthew T G Holden, Brian G Spratt, Androulla Efstratiou, Shiranee Sriskandan   +10 more
doaj   +1 more source

Novel and emerging antimicrobial strategies in the management of oral infections

Periodontology 2000, EarlyView.
Abstract Background Antibiotics marked a pivotal turning point in human civilization, enhancing social interactions and extending human life expectancy. In addition to their success in treating systemic infectious diseases, they have significantly improved periodontal treatment outcomes as an adjunct therapy.
Ozge Unlu, Nil Yakar, Alpdogan Kantarci
wiley   +1 more source

Studying the Oncolytic Activity of Streptococcus pyogenes Strains Against Hepatoma, Glioma, and Pancreatic Cancer In Vitro and In Vivo

Microorganisms
Background: Cancer remains a leading cause of mortality globally. Conventional treatment modalities, including radiation and chemotherapy, often fall short of achieving complete remission, highlighting the critical need for novel therapeutic strategies ...
Anna N. Tsapieva, Alexander N. Chernov, Nadezhda V. Duplik, Anastasiya O. Morozova, Tatiana A. Filatenkova, Mariia A. Suvorova, Elena Egidarova, Elvira S. Galimova, Kseniya Bogatireva, Alexander N. Suvorov   +9 more
doaj   +1 more source