Results 91 to 100 of about 274,580 (343)

Engineered Genetic Circuits Activated by Bezafibrate Improve ESC‐Based TAA Cancer Vaccine Efficacy and PD‐L1 Nanobody Therapy

Advanced Science, EarlyView.
Synthetic gene circuits regulated by bezafibrate are developed to express tumor antigens and PD‐L1 nanobody (PD‐L1nb), enhancing cancer immunotherapy. Delivered via HEK293T cells, ESCs, or directly injected the plasmids containing the circuit into the tumor, the circuits reduce tumor growth, boost CD8+ T cells, and minimize T cell exhaustion.
Meiling Jin, Shuzhen Liu, Mingshuo Zhan, Jian‐Dong Huang   +3 more
wiley   +1 more source

High-resolution yeast actin structures indicate the molecular mechanism of actin filament stiffening by cations

Communications Chemistry
Actin filament assembly and the regulation of its mechanical properties are fundamental processes essential for eukaryotic cell function. Residue E167 in vertebrate actins forms an inter-subunit salt bridge with residue K61 of the adjacent subunit ...
Xiao-Ping Xu, Wenxiang Cao, Mark F. Swift, Nandan G. Pandit, Andrew E. Huehn, Charles V. Sindelar, Enrique M. De La Cruz, Dorit Hanein, Niels Volkmann   +8 more
doaj   +1 more source

Changes in trehalose content of baker's yeast as affected by octanoic acid Alterações no teor de trealose de levedura de panificação provocadas por ácido octanóico

Scientia Agricola, 1993
Octanoic acid inhibited ethanolic fermentation by Saccharomyces cerevisiae (bakers yeast) and the trehalose accumulation, however did not affect the endogenous degradation of trehalose.
L.E. Gutierrez
doaj   +1 more source

Optimisation of recombinant production of active human cardiac SERCA2a ATPase. [PDF]

PLoS ONE, 2013
Methods for recombinant production of eukaryotic membrane proteins, yielding sufficient quantity and quality of protein for structural biology, remain a challenge.
Ana V Antaloae, Cédric Montigny, Marc le Maire, Kimberly A Watson, Thomas L-M Sørensen   +4 more
doaj   +1 more source

Redundancies in Large-scale Protein Interaction Networks [PDF]

arXiv, 2003
Understanding functional associations among genes discovered in sequencing projects is a key issue in post-genomic biology. However, reliable interpretation of the protein interaction data has been difficult. In this work, we show that if two proteins share significantly larger number of common interaction partners than random, they have close ...
arxiv  

Reproducible and Consistent Quantification of the Saccharomyces cerevisiae Proteome by SWATH-mass spectrometry*

Molecular & Cellular Proteomics, 2015
Targeted mass spectrometry by selected reaction monitoring (S/MRM) has proven to be a suitable technique for the consistent and reproducible quantification of proteins across multiple biological samples and a wide dynamic range.
N. Selevsek, Ching-Yun Chang, Ludovic C. Gillet, Pedro Navarro, Oliver M. Bernhardt, L. Reiter, Lin-Yang Cheng, O. Vitek, R. Aebersold   +8 more
semanticscholar   +1 more source

A Unique THN Motif Is Critical for Enabling Efficient C‐Terminal Traceless Cleavage

Advanced Science, EarlyView.
THN is identified as the key motif to facilitate the rapid traceless cleavage of gp41‐1. The deflection of THN motif not only avoids the interaction between C‐exteins and active sites, but also separates Asp107 away from Asn125. In addition, a flexible block F contributes to faster C‐cleavage.
Ruocheng Gu, Yunuo Lin, Rouyu Di, Tongtong Zhou, Tingwen Fan, Wei Li, Lili Miao, Huaiyi Yang   +7 more
wiley   +1 more source

Computer‐aided Evaluation of Polyvalent Medications’ Pharmacological Potential. Multiphytoadaptogen as a Case Study

Molecular Informatics, Volume 42, Issue 1, January 2023., 2023
Abstract Many human diseases including cancer, degenerative and autoimmune disorders, diabetes and others are multifactorial. Pharmaceutical agents acting on a single target do not provide their efficient curation. Multitargeted drugs exhibiting pleiotropic pharmacological effects have certain advantages due to the normalization of the complex ...
O. A. Bocharova, N. S. Ionov, I. V. Kazeev, V. E. Shevchenko, E. V. Bocharov, R. V. Karpova, O. P. Sheychenko, A. A. Aksyonov, S. V. Chulkova, V. G. Kucheryanu, A. V. Revishchin, G. V. Pavlova, V. S. Kosorukov, D. A. Filimonov, A. A. Lagunin, V. B. Matveev, N. V. Pyatigorskaya, I. S. Stilidi, V. V. Poroikov   +18 more
wiley   +1 more source

The Species-Specific Acquisition and Diversification of a K1-like Family of Killer Toxins in Budding Yeasts of the Saccharomycotina.

PLoS Genetics, 2021
Killer toxins are extracellular antifungal proteins that are produced by a wide variety of fungi, including Saccharomyces yeasts. Although many Saccharomyces killer toxins have been previously identified, their evolutionary origins remain uncertain given
Lance R Fredericks, Mark D Lee, Angela M Crabtree, Josephine M Boyer, Emily A Kizer, Nathan T Taggart, Cooper R Roslund, Samuel S Hunter, Courtney B Kennedy, Cody G Willmore, Nova M Tebbe, Jade S Harris, Sarah N Brocke, Paul A Rowley   +13 more
doaj   +1 more source

ZRT1 harbors an excess of nonsynonymous polymorphism and shows evidence of balancing selection in Saccharomyces cerevisiae [PDF]

arXiv, 2012
Estimates of the fraction of nucleotide substitutions driven by positive selection vary widely across different species. Accounting for different estimates of positive selection has been difficult, in part because selection on polymorphism within a species is known to obscure a signal of positive selection between species.
arxiv