Results 51 to 60 of about 274,580 (343)

Saccharomyces cerevisiae: a versatile eukaryotic system in virology

Microbial Cell Factories, 2007
The yeast Saccharomyces cerevisiae is a well-established model system for understanding fundamental cellular processes relevant to higher eukaryotic organisms.
Breinig Tanja, Alves-Rodrigues Isabel, Scheller Nicoletta, Galao Rui P, Meyerhans Andreas, Díez Juana   +5 more
doaj   +1 more source

An outlook to sophisticated technologies and novel developments for metabolic regulation in the Saccharomyces cerevisiae expression system

Frontiers in Bioengineering and Biotechnology, 2023
Saccharomyces cerevisiae is one of the most extensively used biosynthetic systems for the production of diverse bioproducts, especially biotherapeutics and recombinant proteins. Because the expression and insertion of foreign genes are always impaired by
Yijian Wu, Sai Feng, Zeao Sun, Yan Hu, Xiao Jia, Bin Zeng   +5 more
doaj   +1 more source

Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry

Proceedings of the National Academy of Sciences of the United States of America, 2007
We present a strategy for the analysis of the yeast phosphoproteome that uses endo-Lys C as the proteolytic enzyme, immobilized metal affinity chromatography for phosphopeptide enrichment, a 90-min nanoflow-HPLC/electrospray-ionization MS/MS experiment ...
An Chi, C. Huttenhower, Lewis Y. Geer, J. Coon, J. Syka, Dina L. Bai, J. Shabanowitz, D. Burke, O. Troyanskaya, D. Hunt   +9 more
semanticscholar   +1 more source

The Saccharomyces cerevisiae amino acid transporter Lyp1 has a broad substrate spectrum

FEBS Letters, EarlyView.
In Saccharomyces cerevisiae, Yeast Amino acid Transporter family members mediate the import of amino acids, ranging from substrate specialists to generalists. Here, we show that the specialist transporter, Lyp1, has a broader substrate spectrum than previously described, with affinity constants spanning from micromolar to millimolar.
Foteini Karapanagioti, Sebastian Obermaier, Dirk J. Slotboom, Bert Poolman   +3 more
wiley   +1 more source

Applying synthetic biology strategies to bioelectrochemical systems

Electrochemical Science Advances, Volume 2, Issue 6, December 2022., 2022
Abstract Although the past 20 years have seen significant advances in tailoring materials for improving the performance of bioelectrochemical systems, recently, there have been efforts in utilizing the synthetic biology toolkit for engineering organisms for bioelectrochemical systems.
Fangyuan Dong, Olja Simoska, Erin Gaffney, Shelley D. Minteer   +3 more
wiley   +1 more source

KCS1 and VIP1, the genes encoding yeast phosphoinositol pyrophosphate synthases, are required for Ca2+‐mediated response to dimethylsulfoxide (DMSO)

FEBS Open Bio, EarlyView.
Ca2+‐mediated response to DMSO was investigated in Saccharomyces cerevisiae cells expressing Ca2+‐dependent aequorin. Cell exposure to DMSO induced a cytosolic Ca2+ wave dependent on the integrity of the Cch1/Mid1 channel. Deletion of KCS1 or VIP1 genes encoding the phosphoinositol pyrophosphate (PP‐IP) synthases suppressed the DMSO‐induced Ca2 ...
Larisa Ioana Gogianu, Lavinia Liliana Ruta, Claudia Valentina Popa, Simona Ghenea, Ileana Cornelia Farcasanu   +4 more
wiley   +1 more source

Co-evolution of transcriptional silencing proteins and the DNA elements specifying their assembly. [PDF]

PLoS Biology, 2010
Co-evolution of transcriptional regulatory proteins and their sites of action has been often hypothesized but rarely demonstrated. Here we provide experimental evidence of such co-evolution in yeast silent chromatin, a finding that emerged from studies ...
Oliver A Zill, Devin Scannell, Leonid Teytelman, Jasper Rine   +3 more
doaj   +1 more source

Propagation of large concentration changes in reversible protein binding networks [PDF]

Proc Nat Acad Sci U S A 104(34): 13655-13660 (2007), 2007
We study how the dynamic equilibrium of the reversible protein-protein binding network in yeast Saccharomyces cerevisiae responds to large changes in abundances of individual proteins. The magnitude of shifts between free and bound concentrations of their immediate and more distant neighbors in the network is influenced by such factors as the network ...
arxiv   +1 more source

Coordinate control of syntheses of ribosomal ribonucleic acid and ribosomal proteins during nutritional shift-up in Saccharomyces cerevisiae

Molecular and Cellular Biology, 1981
We investigated the regulation of ribosome synthesis in Saccharomyces cerevisiae growing at different rates and in response to a growth stimulus. The ribosome content and the rates of synthesis of ribosomal ribonucleic acid and of ribosomal proteins were
D. R. Kief, J. R. Warner
semanticscholar   +1 more source

Light‐Triggered Protease‐Mediated Release of Actin‐Bound Cargo from Synthetic Cells

Advanced Biology, EarlyView.
TEV Prtoease‐mediated Releasable Actin‐binding Protein (TRAP) is a protein‐based platform consisting of a cargo tightly bound to reconstituted actin networks in synthetic cells which can be proteolyticly released from the bound actin, followed by its secretion through membrane translocation mediated by a cell‐penetrating peptide.
Mousumi Akter, Hossein Moghimianavval, Gary D. Luker, Allen P. Liu   +3 more
wiley   +1 more source