Results 61 to 70 of about 137,997 (266)
Analyzing the Functionality of Non-native Hsp70 Proteins in Saccharomyces cerevisiae
Bio-Protocol, 2019 Yeast are an ideal system to study Heat Shock Protein 70 (Hsp70) function in a cellular context. This protocol was generated to analyze the function of non-native Hsp70 proteins by expressing them as the sole cytosolic Hsp70 in yeast. As an initial step,
Laura Knighton +3 more
doaj +1 more source
Engineering antibodies with cancer‐associated binding sites
BMEMat, EarlyView.This review describes antibodies that perform direct/indirect roles in cancer treatment by binding to effective targets and discusses how antibodies kill tumors and modulate tumor microenvironment through these targets. The classification of expression systems for antibody production is summarized to guide appropriate selection based on different ...
Yinqi Tian +4 more
wiley +1 more source
Impact of bioreactor process parameters and yeast biomass on Raman spectra
Biotechnology Progress, EarlyView.Abstract In‐line Raman spectroscopy combined with chemometric modeling is a valuable process analytical technology (PAT) providing real‐time quantitative information on cell culture compounds. Considering that compound quantification through chemometric models depends on pre‐processing to maintain consistent changes in intensity at certain wavenumbers,
Maarten Klaverdijk +3 more
wiley +1 more source
Identification of Antibacterial Peptide Candidates Encrypted in Stress-Related and Metabolic Saccharomyces cerevisiae Proteins. [PDF]
Pharmaceuticals (Basel), 2022 Santos MFDS +4 more
europepmc +1 more source
Microbial Biotechnology, 2019 Summary Heterologous protein expression in yeast, mostly in Saccharomyces cerevisiae and Pichia pastoris, is a well‐established and widely used technique.
Mario González +3 more
doaj +1 more source
Promising Prodiginins Biological Activities
Chemistry &Biodiversity, EarlyView.ABSTRACT Prodiginins are a large family of at least 34 pyrrolic compounds, including the well‐studied red pigment prodigiosin. Prodiginins are produced by several microorganisms displaying broad biological activities, including antimicrobial, antiviral, antiparasitic, antiproliferative, and immunosuppressive activities.
María F. Ladetto +6 more
wiley +1 more source
Chemistry &Biodiversity, EarlyView.ABSTRACT The subfamily Gomphrenoideae is composed of about 480 accepted species, many of which have been historically used as medicinal plants, reason why they have been studied in terms of chemical profile, biological activity, and safety. This review consolidates the advances in research on this subfamily over the past 47 years, emphasizing its ...
Dayanna Isabel Araque Gelves +3 more
wiley +1 more source
Heterologous prion-forming proteins interact to cross-seed aggregation in Saccharomyces cerevisiae
Scientific Reports, 2017 The early stages of protein misfolding remain incompletely understood, as most mammalian proteinopathies are only detected after irreversible protein aggregates have formed.
Kathryn M. Keefer +2 more
doaj +1 more source
FMN reduces Amyloid-β toxicity in yeast by regulating redox status and cellular metabolism
Nature Communications, 2020 Saccharomyces cerevisiae is a model organism to study proteins involved in neurodegeneration. Here, the authors performed a yeast genome-wide synthetic genetic interaction array (SGA) to screen for toxicity modifiers of Aβ42 and identify riboflavin ...
Xin Chen +6 more
doaj +1 more source
Characterization of the Cubamyces Menziesii Terpenome
ChemBioChem, EarlyView.The genome of Cubamyces menziesii reveals 18 putative sesquiterpene cyclase genes. These genes are cloned and expressed in Escherichia coli, yielding 10 active enzymes. Using farnesyl diphosphate as substrate, the enzymes are analyzed, and the products characterized after bioconversion, uncovering diverse sesquiterpene structures. This study highlights
Létitia Leydet +10 more
wiley +1 more source