Results 61 to 70 of about 254,282 (203)

Studying Spatial Protein Quality Control, Proteopathies, and Aging Using Different Model Misfolding Proteins in S. cerevisiae

Frontiers in Molecular Neuroscience, 2018
Protein quality control (PQC) is critical to maintain a functioning proteome. Misfolded or toxic proteins are either refolded or degraded by a system of temporal quality control and can also be sequestered into aggregates or inclusions by a system of ...
Kara L. Schneider, Thomas Nyström, Per O. Widlund   +2 more
doaj   +1 more source

The PKC, HOG and Ca2+ signalling pathways co-ordinately regulate chitin synthesis in Candida albicans [PDF]

, 2007
Open Access via PMC2649417Peer reviewedPublisher ...
Alistair J. P. Brown, Alonso-Monge R., Bertus Gerssen, Cabib E., Carol A. Munro, Chattaway F.W., De Nobel H., Fonzi W.A., Gooday G.W., Irene de Bruijn, Louise Walker, Megan D. Lenardon, Millar J.B., Mio T., Munro C.A., Munro C.A., Munro C.A., Navarro-Garcia F., Navarro-Garcia F., Neil A. R. Gow, Popolo L., Roncero C., Roncero C., Rose M., Santos B., Sarah Milne, Serena Selvaggini, Sietsma J.H., Sullivan P.A., Uhl M.A., Wang Q., Wessels J.G.H., Ziman M., Ziman M.   +33 more
core   +1 more source

Global Analysis of Protein Sumoylation in Saccharomyces cerevisiae [PDF]

Journal of Biological Chemistry, 2004
Although the modification of cellular factors by SUMO is an essential process in Saccharomyces cerevisiae, the identities of the substrates remain largely unknown. Using a mass spectrometry-based approach, we have identified 271 new SUMO targets. These substrates play roles in a diverse set of biological processes and greatly expand the scope of SUMO ...
James A, Wohlschlegel, Erica S, Johnson, Steven I, Reed, John R, Yates   +3 more
openaire   +2 more sources

Macromolecular protein crystallisation with biotemplate of live cells

Scientific Reports, 2022
Macromolecular protein crystallisation was one of the potential tools to accelerate the biomanufacturing of biopharmaceuticals. In this work, it was the first time to investigate the roles of biotemplates, Saccharomyces cerevisiae live cells, in the ...
Mubai Sun, Huaiyu Yang, Xinyu Miao, Weixian Wang, Jinghui Wang   +4 more
doaj   +1 more source

Regulating repression : roles for the Sir4 N-terminus in linker DNA protection and stabilization of epigenetic states [PDF]

, 2012
The Gasser laboratory is supported by the Novartis Research Foundation and the EU training network Nucleosome 4D. SK was supported by an EMBO long-term fellowship, a Schrodinger fellowship from the FWF, and the Swiss SystemsX.ch initiative/C-CINA; HCF by
Ferreira, Helder C., Gasser, Susan M., Kueng, Stephanie, Oppikofer, Mariano, Roberts, Emma, Roloff, Tim-Christoph, Sack, Ragna, Tsai, Chinyen, Tsai-Pflugfelder, Monika   +8 more
core   +4 more sources

Kcc4 associates with septin proteins of Saccharomyces cerevisiae [PDF]

FEBS Letters, 2001
Kcc4, a kinase of the budding yeast Saccharomyces cerevisiae, is homologous to the bud neck protein kinases Hsl1/Nik1 and Gin4. We report here that a GFP‐Kcc4 fusion protein is localized at the bud neck and that the non‐kinase domain is required for this localization. We also demonstrate that Kcc4 associates with septin proteins in vitro and in vivo by
Okuzaki, Daisuke, Nojima, Hiroshi
openaire   +2 more sources

Saccharomyces cerevisiae as a superior host for overproduction of prokaryotic integral membrane proteins

Current Research in Structural Biology, 2021
Integral membrane proteins (IMPs) constitute ~30% of all proteins encoded by the genome of any organism and Escherichia coli remains the first-choice host for recombinant production of prokaryotic IMPs.
Sarah Spruce Preisler, Anders Drabaek Wiuf, Marc Friis, Lasse Kjaergaard, Molly Hurd, Eva Ramos Becares, Casper Normann Nurup, Frederik Bühring Bjoerkskov, Zsófia Szathmáry, Pontus Emanuel Gourdon, Kirstine Calloe, Dan A. Klaerke, Kamil Gotfryd, Per Amstrup Pedersen   +13 more
doaj   +1 more source

The proteasome lid triggers COP9 signalosome activity during the transition of Saccharomyces cerevisiae cells into quiescence. [PDF]

, 2019
The class of Cullin–RING E3 ligases (CRLs) selectively ubiquitinate a large portion of proteins targeted for proteolysis by the 26S proteasome. Before degradation, ubiquitin molecules are removed from their conjugated proteins by deubiquitinating enzymes,
Bramasole, Lylan, Cirigliano, Angela, Glickman, Michael H., Gurevich, Sylvia, Harshuk, Dana, Pick, Elah, Rinaldi, Teresa, Sinha, Abhishek, Yu, Zanlin   +8 more
core  

Requirement of the FATC domain of protein kinase Tel1 for localization to DNA ends and target protein recognition [PDF]

, 2015
Two large phosphatidylinositol 3-kinase-related protein kinases (PIKKs), ATM and ATR, play a central role in the DNA damage response pathway. PIKKs contain a highly conserved extreme C-terminus called the FRAP-ATM-TRRAP-C-terminal (FATC) domain.
Ghosh, A., Goto, G.H., Henry, E., Ogi, H., Sugimoto, K., Zencir, Sevil   +5 more
core   +1 more source

Characterising Maturation of GFP and mCherry of Genomically Integrated Fusions in Saccharomyces cerevisiae

Bio-Protocol, 2018
Single-molecule fluorescence microscopy enables unrivaled sub-cellular quantitation of genomically encoded fusions of native proteins with fluorescent protein reporters.
Sviatlana Shashkova, Adam Wollman, Stefan Hohmann, Mark C Leake   +3 more
doaj   +1 more source