Results 41 to 50 of about 10,552 (197)

A duplex droplet digital PCR assay for absolute quantification and characterization of long self-amplifying RNA

Scientific Reports, 2023
Self-amplifying messenger ribonucleic acid (saRNA) provides extended expression of genes of interest by encoding an alphavirus-derived RNA replicase and thus is 2–3 times larger than conventional messenger RNA.
Irafasha C. Casmil, Cynthia Huang, Anna K. Blakney   +2 more
doaj   +1 more source

Biophysical characterization of the structure of a SARS-CoV-2 self-amplifying RNA (saRNA) vaccine

Biology Methods and Protocols, 2023
The current SARS-Covid-2 (SARS-CoV-2) pandemic has led to an acceleration of messenger ribonucleic acid (mRNA) vaccine technology. The development of production processes for these large mRNA molecules, especially self-amplifying mRNA (saRNA), has ...
Daniel P Myatt, Lewis Wharram, C. Graham, J. Liddell, H. Branton, C. Pizzey, N. Cowieson, R. Rambo, R. Shattock   +8 more
semanticscholar   +1 more source

p21CIP/WAF1 saRNA inhibits proliferative vitreoretinopathy in a rabbit model

PLoS ONE, 2023
Purpose Proliferative vitreoretinopathy (PVR) is a disease process resulting from proliferation of retinal pigment epithelial (RPE) cells in the vitreous and periretinal area, leading to periretinal membrane formation and traction and eventually to ...
Qi Zhang, Yangchen Guo, Moorim Kang, Wei-Hsiang Lin, Jian-Cheng Wu, Yi Yu, Long-Cheng Li, Aimin Sang   +7 more
semanticscholar   +1 more source

A self-amplifying RNA vaccine against COVID-19 with long-term room-temperature stability

npj Vaccines, 2022
mRNA vaccines were the first to be authorized for use against SARS-CoV-2 and have since demonstrated high efficacy against serious illness and death. However, limitations in these vaccines have been recognized due to their requirement for cold storage ...
Emily A. Voigt, Alana Gerhardt, Derek Hanson, Madeleine F. Jennewein, Peter Battisti, Sierra Reed, Jasneet Singh, Raodoh Mohamath, Julie Bakken, Samuel Beaver, Christopher Press, Patrick Soon-Shiong, Christopher J. Paddon, Christopher B. Fox, Corey Casper   +14 more
doaj   +1 more source

Heterologous saRNA Prime, DNA Dual-Antigen Boost SARS-CoV-2 Vaccination Elicits Robust Cellular Immunogenicity and Cross-Variant Neutralizing Antibodies

bioRxiv, 2022
We assessed if immune responses are enhanced in CD-1 mice by heterologous vaccination with two different nucleic acid-based COVID-19 vaccines: a next-generation human adenovirus serotype 5 (hAd5)-vectored dual-antigen spike (S) and nucleocapsid (N ...
A. Rice, M. Verma, Emily A. Voigt, Peter Battisti, Sam Beaver, Sierra Reed, K. Dinkins, Shivani Mody, L. Zakin, Shiho Tanaka, B. Morimoto, C. A. Olson, E. Gabitzsch, J. Safrit, P. Spilman, C. Casper, P. Soon-Shiong   +16 more
semanticscholar   +1 more source

OX40 agonist stimulation increases and sustains humoral and cell-mediated responses to SARS-CoV-2 protein and saRNA vaccines

Frontiers in Immunology, 2022
To prevent SARS-CoV-2 infections and generate long-lasting immunity, vaccines need to generate strong viral-specific B and T cell responses. Previous results from our lab and others have shown that immunizations in the presence of an OX40 agonist ...
Rebekka Duhen, Michael Beymer, S. Jensen, Srinivas Abbina, Suraj Abraham, Nikita Jain, A. Thomas, A. Geall, Hong-Ming Hu, B. Fox, A. Weinberg   +10 more
semanticscholar   +1 more source

Nazwy apelatywne dotyczące sarny w polskim i rosyjskim socjolekcie łowieckim – nazwy gatunkowe, nazwy określające płeć oraz nazwy istot młodych

Annales Universitatis Paedagogicae Cracoviensis. Studia Linguistica, 2023
The aim of the article is to present the terms concerning a roe‑deer used by hunters in the 21st century. There were discussed the names of spices living in Poland and living in Russia, names specifying the sex of the animal and names of its offspring ...
Beata Malczewska, Joanna Smoła
doaj   +1 more source

Reverse-phase chromatography removes double-stranded RNA, fragments, and residual template to decrease immunogenicity and increase cell potency of mRNA and saRNA

Molecular Therapy: Nucleic Acids
mRNA is produced by in vitro transcription reaction, which also leads to formation of immuno-stimulatory impurities, such as double-stranded RNA (dsRNA). dsRNA leads to activation of innate immune response linked to inhibition of protein synthesis.
Andreja Krušič, Nina Mencin, Marta Leban, Evelin Nett, Mario Perković, Uğur Şahin, Polona Megušar, A. Štrancar, Rok Sekirnik   +8 more
semanticscholar   +1 more source

Situación actual de la sarna e infecciones parasitarias en vicuñas (Vicugna vicugna) de la Región Cusco, Perú

Revista de Investigaciones Veterinarias del Perú, 2021
El objetivo del presente estudio fue identificar y evaluar los casos de sarna causada por Sarcoptes scabiei y de endoparásitos en vicuñas silvestres y en cautiverio (Vicugna vicugna) de la Región Cusco, Perú.
José M. Angulo-Tisoc, J. Pacheco, Víctor Vélez, Wilber García, Henry Castelo, Luis A. Gómez-Puerta   +5 more
semanticscholar   +1 more source

Tetrahedral Framework Nucleic Acid‐Based Delivery of DJ‐1‐saRNA Prevent Retinal Ischaemia–Reperfusion Injury via Inhibiting Ferroptosis

Cell Proliferation
Retinal ischaemia/reperfusion injury (RI/RI) is the primary pathophysiological mechanism underlying retinal ischaemic diseases, potentially resulting in significant and irreversible visual impairment.
Xianggui Zhang, Zhen-De Deng, Xiaoxiao Xu, Jingyi Zhu, Zhen Huang, Ya Ye, Jingying Liu, Delun Luo, Jinnan Liu, Ming Yan, Yanping Song   +10 more
semanticscholar   +1 more source