Results 51 to 60 of about 10,552 (197)

Laminar fluid ejection device enables high yield and preservation of mRNA and SaRNA LNP formulations

Scientific Reports
The development of messenger RNA (mRNA) and self-amplifying RNA (saRNA) vaccines has revolutionized modern vaccinology, particularly with the success of lipid nanoparticle (LNP)-based SARS-CoV-2 vaccines.
Chiao-Hao Ho, Irafasha C. Casmil, Manu Sharma, Tim Rees, Kenza Enright, Nick Allan, A. Blakney   +6 more
semanticscholar   +1 more source

Infestação por ácaros da espécie Chirodiscoides caviae em porquinho-da-Índia (Cavia porcellus)

Medicina Veterinária, 2023
A espécie Cavia porcellus (Rodentia, Caviidae), conhecida popularmente como cobaia ou porquinho-da-Índia, é um roedor utilizado como animal de laboratório e, devido ao seu tamanho e fácil adaptação, vem ganhando maior espaço nos lares brasileiros como ...
Rayssa Dourado Fontenele, Luiz Fernando Wolpert de Gois, Clara Cecília Azevedo Santana, Marcos Renan Barbosa Reis, Raíssa Esthephane Torres do Nascimento, Águida Teresa Rabelo da Silva, Mariana Sousa Ribeiro, Luanna Soares de Melo Evangelista   +7 more
doaj   +4 more sources

Impact of B18R-Encoding Messenger Ribonucleic Acid Co-Delivery on Neutralizing Antibody Production in Self-Amplifying Messenger Ribonucleic Acid Vaccines

Vaccines
Objectives: The COVID-19 pandemic has brought mRNA vaccines to the forefront due to their widespread use. In this study, we explored the potential advantages of the self-amplifying mRNA (saRNA) vaccine over conventional mRNA vaccines. Methods: Initially,
Yutao Wang, Lei Li, Min Liang, Gan Liu, Yinying Lu   +4 more
doaj   +1 more source

An Update on Self-Amplifying mRNA Vaccine Development

Vaccines, 2021
This review will explore the four major pillars required for design and development of an saRNA vaccine: Antigen design, vector design, non-viral delivery systems, and manufacturing (both saRNA and lipid nanoparticles (LNP)).
Anna K. Blakney, Shell Ip, Andrew J. Geall   +2 more
doaj   +1 more source

Concerted Action of Targeted Nucleic Acid Therapeutics as Flexible, Precision and Personalized Cancer Treatment

Advanced Science, EarlyView.
Combinations of small activating RNAs and small interfering RNAs were developed as personalized precision therapies that simultaneously activate tumor suppressors and silence oncogenes according to the molecular signatures of patient tumors. This combination strategy demonstrated superior anticancer efficacy, highlighting the promise of precision ...
Jing Wu, Jiaxuan Chen, Abdechakour Elkihel, Wenjun Lan, Nicolas Fraunhoffer, Dandan Zhu, Baoping Lian, Christina Galanakou, Zhancun Bian, Xi Liu, Odile Gayet, Loic Moubri, Julie Aglietti Roques, Vikash Reebye, Nagy Habib, Xiaoxuan Liu, Nelson Dusetti, Juan Lucio Iovanna, Ling Peng   +18 more
wiley   +1 more source

A Novel Self‐Amplifying mRNA with Decreased Cytotoxicity and Enhanced Protein Expression by Macrodomain Mutations

Advanced Science
The efficacy and safety of self‐amplifying mRNA (saRNA) have been demonstrated in COVID‐19 vaccine applications. Unlike conventional non‐replicating mRNA (nrmRNA), saRNA offers a key advantage: its self‐replication mechanism fosters efficient expression ...
Yue Gong, Danni Yong, Gensheng Liu, Jiang Xu, Jun Ding, William Jia   +5 more
doaj   +1 more source

Dysregulation of the MACF1‐Rab14/KIF16B‐FGFR Vesicular Trafficking Axis Skews MSC Lineage Commitment in Glucocorticoid‐Induced Osteoporosis

Advanced Science, EarlyView.
Under normal conditions, MACF1 interacts with Rab14 and facilitates KIF16B‐mediated FGFR vesicle trafficking along microtubules to the plasma membrane, thereby supporting BMSC differentiation. In contrast, chronic GC exposure suppresses MACF1 expression, disrupting this transport and causing intracellular FGFR retention, which blunts osteogenic ...
Peihong Su, Ye Tian, Fan Zhao, Jing Li, Suryaji Patil, Chunyu Zhu, Yujia Tian, Junrong Wang, Jiating Liu, Aoqi Xiang, Hua Guan, Lusha Zhang, Xiaochang Chen, Airong Qian, Qi Yu   +14 more
wiley   +1 more source

Co‐expression of HSV‐1 ICP34.5 enhances the expression of gene delivered by self‐amplifying RNA and mitigates its immunogenicity

FEBS Open Bio
Self‐amplifying RNA (saRNA) vectors have garnered significant attention for their potential in transient recombinant protein expression and vaccination strategies.
Xuemin Lu, Yabin Wu, Chunye Zhao, Jie Zheng, Shangwu Chen, Yigang Wang, Yulong Xia   +6 more
doaj   +1 more source

A review on Gaucher disease: therapeutic potential of β-glucocerebrosidase-targeted mRNA/saRNA approach

International Journal on Biological Sciences
Gaucher disease (GD), a rare hereditary lysosomal storage disorder, occurs due to a deficiency in the enzyme β-glucocerebrosidase (GCase). This deficiency leads to the buildup of substrate glucosylceramide (GlcCer) in macrophages, eventually resulting in
Shunping Feng, N. Rcheulishvili, Xiaoming Jiang, Pan Zhu, Xuehua Pan, Meilan Wei, P. Wang, Yang Ji, Dimitri Papukashvili   +8 more
semanticscholar   +1 more source

Continuous Purification of mRNA Produced by In Vitro Transcription Using High Performance Countercurrent Membrane Purification

Biotechnology and Bioengineering, EarlyView.
ABSTRACT Messenger ribonucleic acid (mRNA) therapeutics produced by in vitro transcription must be purified to remove residual enzymes and free nucleotides. This study examines the use of high‐performance countercurrent membrane purification (HPCMP) for the purification of mRNA therapeutics based on differences in the rate of diffusion across ...
Ziqiao Wang, Amin Javidanbardan, Ali Behboudi, Andrew L. Zydney   +3 more
wiley   +1 more source