Results 111 to 120 of about 2,100,013 (302)

A light‐triggered Time‐Resolved X‐ray Solution Scattering (TR‐XSS) workflow with application to protein conformational dynamics

open access: yesFEBS Open Bio, EarlyView.
Time‐resolved X‐ray solution scattering captures how proteins change shape in real time under near‐native conditions. This article presents a practical workflow for light‐triggered TR‐XSS experiments, from data collection to structural refinement. Using a calcium‐transporting membrane protein as an example, the approach can be broadly applied to study ...
Fatemeh Sabzian‐Molaei   +3 more
wiley   +1 more source

A branch-and-bound algorithm for stable scheduling in single-machine production systems. [PDF]

open access: yes
Robust scheduling aims at the construction of a schedule that is protected against uncertain events. A stable schedule is a robust schedule that will change little when variations in the input parameters arise.
Herroelen, Willy, Leus, Roel
core  

Multi-Project Staff Scheduling Optimization Considering Employee Welfare in Construction Projects

open access: yesBuildings
Multi-project staff scheduling optimization is a critical challenge in engineering management. However, traditional methods often overlook employee welfare, resulting in scheduling solutions that lack a people-centered approach.
Junlong Peng, Fei Zhou
doaj   +1 more source

Analysing the significance of small conformational changes and low occupancy states in serial crystallographic data

open access: yesFEBS Open Bio, EarlyView.
This protocol paper outlines methods to establish the success of a time‐resolved serial crystallographic experiment, by means of statistical analysis of timepoint data in reciprocal space and models in real space. We show how to amplify the signal from excited states to visualise structural changes in successful experiments.
Jake Hill   +4 more
wiley   +1 more source

A hybrid scatter search. Electromagnetism meta-heuristic for project scheduling. [PDF]

open access: yes
In the last few decades, several effective algorithms for solving the resource-constrained project scheduling problem have been proposed. However, the challenging nature of this problem, summarised in its strongly NP-hard status, restricts the ...
Debels, D   +3 more
core  

Power Battery Scheduling Optimization Based on Deep Deterministic Strategy Gradient Algorithm With Bayesian Optimization

open access: yesIEEE Access
Under the promotion of the national new energy strategy, the utilization rate of renewable energy is steadily increasing, and the application scale of power batteries is growing.
Yue Li   +6 more
doaj   +1 more source

Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens

open access: yesFEBS Open Bio, EarlyView.
Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee   +3 more
wiley   +1 more source

Refinement of amino‐acid conformation vs. difference density maps in time‐resolved serial femtosecond crystallography data analysis

open access: yesFEBS Open Bio, EarlyView.
The dFoCC pipeline starts with observed DED and resting‐state coordinates, which are then used to generate a library of triggered states. Correlation analysis of the calculated DED features of each candidate vs observed DED permits quantitative evaluation of candidate structural quality.
Meng Iao Fong   +3 more
wiley   +1 more source

Versatile vector tools for efficient protein screening across multiple expression systems

open access: yesFEBS Open Bio, EarlyView.
A unified vector toolkit enables rapid protein expression screening across E. coli, insect, and mammalian cells. A single primer pair amplifies the target gene, which is inserted into any vector via a standardized interface. This streamlined workflow eliminates repeated cloning steps, accelerating the identification of optimal expression conditions for
Zhimin Zhu   +5 more
wiley   +1 more source

'Explicit Learning: an Effort towards Human Scheduling Algorithms' [PDF]

open access: yes, 2003
Scheduling problems are generally NP-hard combinatorial problems, and a lot of research has been done to solve these problems heuristically. However, most of the previous approaches are problem-specific and research into the development of a general ...
Aickelin, Uwe   +3 more
core  

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