Results 51 to 60 of about 69 (69)

Schizosaccharomyces pombe Isolation Protocol

2018
This chapter describes a methodology to isolate yeast strains from Schizosaccharomyces pombe species. The method is based on a selective-differential medium that notably facilitates the isolation of S. pombe. The main difficulty in isolating microorganisms from this genus is their extremely low incidence in nature when they are compared to other ...
Ángel Benito, Fernando Calderón, Santiago Benito   +2 more
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Overview of Schizosaccharomyces pombe

Current Protocols in Molecular Biology, 2003
AbstractThe fission yeast S. pombe provides an attractive alternative system to budding yeast S. cerevisiae for studies of fundamental cell biology. Fission yeast is a particularly powerful model for studies of cell cycle, chromosome dynamics, and polarity. Other areas of study are also expanding.
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Molecular Genetics of Schizosaccharomyces pombe

2010
In this chapter we present basic protocols for the use of Schizosaccharomyces pombe, commonly known as fission yeast, in molecular biology and genetics research. Fission yeast is an increasingly popular model organism for the study of biological pathways because of its genetic tractability and as a model for metazoan biology. It provides an alternative
Susan L. Forsburg, Sarah A. Sabatinos
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Pac1 Ribonuclease of Schizosaccharomyces pombe

2001
Publisher Summary The Pac1 RNase of the fission yeast Schizosaccharomyces pombe is a doublestrand-specific endoribonuclease (dsRNase) whose structure, biochemical properties, and biological functions place it in the RNase III family. This chapter describes the methods that have been used to define the biological functions and biochemical properties ...
David Frendewey, G. Rotondo
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Cnb1 involved in cytokinesis in Schizosaccharomyces pombe

Hereditas (Beijing), 2013
Serine/Threonine-specific calcineurin (CN) is highly conserved in eukaryotes, which plays an important role in transcriptional regulation. In Schizosaccharomyces pombe, CN exists as a heterodimer composed by catalytic subunit Ppb1 and regulatory subunit Cnb1.
Bi-Wei Feng, Yao Yu, Ji-Feng Wang, Jie-Qiong Fan, Xiao-Long Deng, Hong Lv   +5 more
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Genetic Analysis of Schizosaccharomyces pombe

Cold Spring Harbor Protocols, 2017
In this introduction we discuss some basic genetic tools and techniques that are used with the fission yeast Schizosaccharomyces pombe. Genes commonly used for selection or as reporters are discussed, with an emphasis on genes that permit counterselection, intragenic complementation, or colony-color assays. S. pombe is most stable as a haploid organism.
Ekwall, Karl, Thon, Genevieve
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Selecting Schizosaccharomyces pombe Diploids

Cold Spring Harbor Protocols, 2017
Here we describe procedures for the selection of diploid Schizosaccharomyces pombe. ade6-M210/ade6-M216 heteroallelic complementation is widely used to select for Ade+ diploids. Such diploids will readily sporulate when starved of nitrogen. For some investigations, stable diploids are preferable (e.g., for genetic complementation tests), and in these ...
Ekwall, Karl, Thon, Genevieve
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CSL proteins of Schizosaccharomyces pombe

2009
The CSL family of transcription factors is essential for metazoan development,mostly due to their involvement in the Notch signaling pathway. We identified two novel classes of CSL genes in several fungal species, organisms lacking the Notch pathway. We characterized experimentally cbfl I- and cbfl2*, the two CSL genesof Schizosaccharomyces pombe, in ...
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THE CYTOLOGY OF MEIOSIS IN SCHIZOSACCHAROMYCES OCTOSPORUS

American Journal of Botany, 1955
IN 1894 BEYERINCK discovered the yeast Schizosaccharomyces octosporus and described its optimum growth conditions and gross morphology. He described the nucleus of the young ascus as a small, transparent, undifferentiated body lying in the thick protoplasmic area of the cell, and noted the difficulty of distinguishing it from the small vacuoles and ...
Edward D. DeLamater, Abraham Widra
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Chapter 7 Preparation of Protoplasts of Schizosaccharomyces pombe

1978
Publisher Summary The chapter describes a method by which cells of S. pombe are completely and efficiently converted into protoplasts. The method requires a mixture of α-1,3-glucanase,exo-β-1, 3 glucanase and snail enzymes. The quantitative preparation of protoplasts from two strains of Schizosacchuromyces by the combined action of snail gut ...
J Schwencke, M Nagy
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