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Overview of Schizosaccharomyces pombe
Current Protocols in Molecular Biology, 2003AbstractThe fission yeast S. pombe provides an attractive alternative system to budding yeast S. cerevisiae for studies of fundamental cell biology. Fission yeast is a particularly powerful model for studies of cell cycle, chromosome dynamics, and polarity. Other areas of study are also expanding.
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Molecular Genetics of Schizosaccharomyces pombe
2010In this chapter we present basic protocols for the use of Schizosaccharomyces pombe, commonly known as fission yeast, in molecular biology and genetics research. Fission yeast is an increasingly popular model organism for the study of biological pathways because of its genetic tractability and as a model for metazoan biology. It provides an alternative
Sarah A, Sabatinos, Susan L, Forsburg
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Pac1 Ribonuclease of Schizosaccharomyces pombe
2001Publisher Summary The Pac1 RNase of the fission yeast Schizosaccharomyces pombe is a doublestrand-specific endoribonuclease (dsRNase) whose structure, biochemical properties, and biological functions place it in the RNase III family. This chapter describes the methods that have been used to define the biological functions and biochemical properties ...
G, Rotondo, D, Frendewey
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The Nutrition of Schizosaccharomyces Pombe
Mycologia, 1955Yeasts have been, for almost a century, the bases of investigations leading to fundamental discoveries which have become the foundation upon which much of modern biological science has been built. Accord? ing to R. J. Williams (1941), "Some of the most far-reaching dis?
Ilda McVeigh, Everett Bracken
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X-ray tomography of Schizosaccharomyces pombe
Differentiation, 2007The genetic tractability of the unicellular yeast Schizosaccharomyces pombe has resulted in it becoming an important model organism for the study of many eukaryotic cellular processes, in particular cell division. Over the past few years much progress has been made toward understanding the mechanisms that regulate eukaryotic cell division and the ...
Weiwei, Gu +3 more
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[RNAi and the formation of heterochromatin in Schizosaccharomyces pombe.].
Journal de la Societe de biologie, 2007In the fission yeast Schizosaccharomyces pombe, formation of pericentromeric heterochromatin involves RNA interference (RNAi). Recent data indicate that two RNAi complexes, RITS (RNA-induced transcriptional silencing complex) and RDRC (RNA-directed RNA polymerase complex), their respective enzymatic activity, and RNA polymerase II are essential for ...
Barral, Sophie +2 more
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Recovery, Repair, and Mutagenesis in Schizosaccharomyces pombe
1985Publisher Summary This chapter presents an account of recovery, repair, and mutation processes in the fission yeast, Schizosaccharomyces pombe . Some of the aspects such as the cell cycle, macromolecular synthesis, and cell synchrony are documented to give an idea of the potential and experimental usefulness of S. pombe .
Phipps, Jenny, Nasim, A., Miller, D.
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The ypt proteins of Schizosaccharomyces pombe
Biochemical Society Transactions, 199465 66 Mayer, B. J., Ren, K., Clark, K. I,. and Baltimore, D. (1993) Cell 73,629-630 Haslarn, K. J., Koide, H. B. and Hemmings, B. A. (1993) Nature (London) 363,309-310 Musacchio, A., Gibson, T., Price, P., Thompson, J. and Saraste, M. (1993) Trends Biochem. Sci. 18, 343-348 Lefkowitz, R. J. (1993) Cell 74,409-412 Bourne, H. K., Sanders, D. A.
J, Armstrong +5 more
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Cell cycle regulation in Schizosaccharomyces pombe
Current Opinion in Microbiology, 2000Cdc2, a cyclin-dependent kinase, controls cell cycle progression in fission yeast. New details of Cdc2 regulation and function have been uncovered in recent studies. These studies involve cyclins that associate with Cdc2 in G1-phase and the proteins that regulate inhibitory phosphorylation of Cdc2 during S-phase and G2-phase. Recent investigations have
B A, Moser, P, Russell
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A study of integrative transformation in Schizosaccharomyces pombe
Molecular and General Genetics MGG, 1993Using the one-step gene disruption technique, we studied the effect of various parameters on the disruption frequency (percentage of homologous integrants) and transformation efficiency (number of transformants per microgram of input DNA) of integrative transformation in Schizosaccharomyces pombe. We used suc1 as the target gene for disruption and ura4
B, Grallert, P, Nurse, T E, Patterson
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