Sensitive and Quantitative Three-Color Protein Imaging in Fission Yeast Using Spectrally Diverse, Recoded Fluorescent Proteins with Experimentally-Characterized In Vivo Maturation Kinetics. [PDF]
Schizosaccharomyces pombe is an outstanding model organism for cell biological investigations, yet the range of useful and well-characterized fluorescent proteins (XFPs) is limited.
Bassem Al-Sady +4 more
doaj +1 more source
Extensive mass spectrometry-based analysis of the fission yeast proteome: the Schizosaccharomyces pombe PeptideAtlas [PDF]
We report a high quality and system-wide proteome catalogue covering 71% (3,542 proteins) of the predicted genes of fission yeast, Schizosaccharomyces pombe, presenting the largest protein dataset to date for this important model organism.
Schmidt, A +29 more
core +1 more source
Investigation of RNA-induced gene silencing in the fission yeast Schizosaccharomyces pombe [PDF]
PhDRNA interference (RNAi) is a gene regulating system to which can silence genes at both transcriptional and post-transcriptional levels. In this thesis, a ura4-based RNAi selective assay was developed in the fission yeast Schizosaccharomyces pombe ...
Wang, Huan
core +2 more sources
The DNA-binding domain of S. Pombe Mrc1 (Claspin) acts to enhance stalling at replication barriers [PDF]
Funding: Marie Curie Cancer Care (J.Z. and J.Z.D.), Biotechnology and Biological Sciences Research Council (J.Z.D.) and Cancer Research UK (E.H.) supported this work.During S-phase replication forks can stall at specific genetic loci.
Dalgaard, J.Z. +20 more
core +1 more source
The cell cycle-regulated genes of Schizosaccharomyces pombe. [PDF]
Many genes are regulated as an innate part of the eukaryotic cell cycle, and a complex transcriptional network helps enable the cyclic behavior of dividing cells.
Anna Oliva +7 more
doaj +2 more sources
Analysis of Rad3 and Chk1 protein kinases defines different checkpoint responses. [PDF]
Eukaryotic cells respond to DNA damage and S phase replication blocks by arresting cell-cycle progression through the DNA structure checkpoint pathways.
Lindsay, Howard D. +6 more
core +3 more sources
Possible involvement of nitric oxide and reactive oxygen species in glucose deprivation-induced activation of transcription factor rst2. [PDF]
Glucose is one of the most important sources of cellular nutrition and glucose deprivation induces various cellular responses. In Schizosaccharomyces pombe, zinc finger protein Rst2 is activated upon glucose deprivation, and regulates gene expression via
Toshiaki Kato, Xin Zhou, Yan Ma
doaj +1 more source
Genetic engineering of
A commercial use of microbial produced products, like polyhydroxyalkanotes (PHAs), in the sense of an environmental precaution appears meaningful and necessary.
AT Buelhamd +4 more
doaj +1 more source
DNA polymerase α (swi7) and the flap endonuclease fen1 (rad2) act together in the s-phase alkylation damage response in S. pombe [PDF]
Polymerase α is an essential enzyme mainly mediating Okazaki fragment synthesis during lagging strand replication. A specific point mutation in Schizosaccharomyces pombe polymerase α named swi7-1, abolishes imprinting required for mating-type switching ...
Koulintchenko Milana +20 more
core +2 more sources
Biochemical characterization and DNA repair pathway interactions of Mag1-mediated base excision repair in Schizosaccharomyces pombe [PDF]
The Schizosaccharomyces pombe mag1 gene encodes a DNA repair enzyme with sequence similarity to the AlkA family of DNA glycosylases, which are essential for the removal of cytotoxic alkylation products, the premutagenic deamination product hypoxanthine ...
Alseth, I +6 more
core +1 more source

