Results 61 to 70 of about 28,351 (178)
Systematic analysis reveals the prevalence and principles of bypassable gene essentiality
Nature Communications, 2019 An essential gene may become non-essential when another gene is mutated. Here, the authors investigate this type of digenic interaction, termed ‘bypass of essentiality’, in the fission yeast Schizosaccharomyces pombe, and show that bypassable essential ...
Jun Li +10 more
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Transposons as Tools for Future Genome Engineering in Yeasts and Filamentous Fungi
Yeast, EarlyView.Applications of transposon‐based tools for genome engineering in fungi include transposon‐mediated mutagenesis and targeted genomic integration. ABSTRACT Transposons are fundamental genetic elements that have profoundly shaped the architecture of eukaryotic genomes.
Bingyin Peng +4 more
wiley +1 more source
Growth and the Environment of Schizosaccharomyces pombe [PDF]
Cold Spring Harbor Protocols, 2016 Here, we summarize the composition and uses of Schizosaccharomyces pombe media and discuss key issues for consideration in the generation of S. pombe cultures. We discuss the concept of “culture memory,” in which the growth state and stress experienced by a strain during storage, propagation, and starter culture preparation can alter experimental ...
Janni, Petersen, Paul, Russell
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CRL4Wdr70 regulates H2B monoubiquitination and facilitates Exo1-dependent resection
Nature Communications, 2016 The repair of double-strand DNA breaks by homologous recombination requires resection of the DNA ends. Here the authors show that in Schizosaccharomyces pombe and human cells, Wdr70 is recruited as part of the CRL4 complex to promote ubiquitination of ...
Ming Zeng +12 more
doaj +1 more source
Population genomics of the fission yeast Schizosaccharomyces pombe. [PDF]
PLoS ONE, 2014 The fission yeast Schizosaccharomyces pombe has been widely used as a model eukaryote to study a diverse range of biological processes. However, population genetic studies of this species have been limited to date, and we know very little about the ...
Jeffrey A Fawcett +10 more
doaj +1 more source
The FEBS Journal, EarlyView.Pyridoxal 5′‐phosphate (PLP) homeostasis relies on salvage enzymes, yet key metabolic branches remain undefined. We identify AKR1C isozymes as previously undescribed contributors that convert pyridoxal into pyridoxine or 4‐pyridoxolactone through reductase and dehydrogenase activities.
Nayu Kito +8 more
wiley +1 more source
Studies on the Transglucosidation of Schizosaccharomyces pombe
Journal of the agricultural chemical society of Japan, 1955 グルコース及び1,4-α-結合のpolymerからなる水飴にSchizosaccharomyces Pombeを作用させ転移生成するsakebiose, kojibiose, isomaltose, panose, isomaltotriose, kojitriose及び残存maltose, maltotriose等を主にcarbon column chromatographyで分離,遊離糖或いはアセテートとして結晶にとり確認した.
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Optimisation of the Schizosaccharomyces pombe urg1 expression system. [PDF]
PLoS ONE, 2013 The ability to study protein function in vivo often relies on systems that regulate the presence and absence of the protein of interest. Two limitations for previously described transcriptional control systems that are used to regulate protein expression
Adam T Watson +6 more
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The FEBS Journal, EarlyView.Homologous recombination (HR) in Schizosaccharomyces pombe is essential when mating‐type switching–induced double‐strand breaks (DSBs) form at the mat1 locus. In h90 and h− strains, efficient mat1 DSB formation renders HR indispensable unless suppressors block these breaks.
Peter Kolesar +3 more
wiley +1 more source
Mobile DNA, 2020 Genomics and other large-scale analyses have drawn increasing attention to the potential impacts of transposable elements (TEs) on their host genomes. However, it remains challenging to transition from identifying potential roles to clearly demonstrating
Patrick H. Maxwell
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