Results 61 to 70 of about 55,120 (213)

A eubacterial origin for the human tRNA nucleotidyltransferase? [PDF]

, 2001
tRNA CCA-termini are generated and maintained by tRNA nucleotidyltransferases. Together with poly(A) polymerases and other enzymes they belong to the nucleotidyltransferase superfamily.
Aebi M., Altschul S.F., Chen J.Y., Cudny H., Deutscher M.P., Deutscher M.P., Deutscher M.P., Deutscher M.P., Martin G., Raynal L.C., Saitou N., Yue D., Zhu L.   +12 more
core   +1 more source

A new branch of mammalian vitamin B6 metabolism: AKR1C‐mediated conversion of pyridoxal to pyridoxine and 4‐pyridoxolactone

The FEBS Journal, EarlyView.
Pyridoxal 5′‐phosphate (PLP) homeostasis relies on salvage enzymes, yet key metabolic branches remain undefined. We identify AKR1C isozymes as previously undescribed contributors that convert pyridoxal into pyridoxine or 4‐pyridoxolactone through reductase and dehydrogenase activities.
Nayu Kito, Yasuyuki Kitaura, Kyoka Iino, Kazuya Toriumi, Hyunah Noh, Naoya Ogawa, Makoto Arai, Hisashi Hemmi, Tomokazu Ito   +8 more
wiley   +1 more source

Identification and functional analysis of the erh1(+) gene encoding enhancer of rudimentary homolog from the fission yeast Schizosaccharomyces pombe. [PDF]

PLoS ONE, 2012
The ERH gene encodes a highly conserved small nuclear protein with a unique amino acid sequence and three-dimensional structure but unknown function. The gene is present in animals, plants, and protists but to date has only been found in few fungi.
Marek K Krzyzanowski, Ewa Kozlowska, Piotr Kozlowski   +2 more
doaj   +1 more source

Silver nanoparticles elevate mutagenesis of eukaryotic genomes

G3: Genes, Genomes, Genetics, 2023
Metal nanoparticles, especially silver, have been used in various medical scenarios, due to their excellent antimicrobial effects. Recent studies have shown that AgNPs do not exert mutagenic effects on target bacteria, but the degree to which they ...
Kun Wu, Haichao Li, Yaohai Wang, Dan Liu, Hui Li, Yu Zhang, Michael Lynch, Hongan Long   +7 more
doaj   +1 more source

Quantification of DNA-associated proteins inside eukaryotic cells using single-molecule localization microscopy [PDF]

, 2014
Development of single-molecule localization microscopy techniques has allowed nanometre scale localization accuracy inside cells, permitting the resolution of ultra-fine cell structure and the elucidation of crucial molecular mechanisms.
Adam T. Watson, Alex Herbert, Antony M. Carr, Betzig, Bähler, Culbertson, David Klenerman, David Lando, Dion, Elf, Ernest Laue, Forsburg, Heilemann, Henteges, Hess, Horrocks, Jem Tucker, Kearsey, Kim, Krichevsky, Kubota, Lando, Manley, Mark A. Osborne, Masai, Matsuyama, Matthieu Palayret, Meister, Michalet, Mortensen, Patterson, Peter Jönsson, Reyes-Lamothe, Rust, Rémi L. Boulineau, Shiomi, Sophie George, Spendier, Steven F. Lee, Stracy, Su'etsugu, Szymborska, Thomas J. Etheridge, Tokunaga, Uphoff, Watson, Yasukazu Daigaku   +46 more
core   +1 more source

Homologous recombination mutants cause differing lethality between h− and h+ Schizosaccharomyces pombe strains due to mat1 heterochromatin

The FEBS Journal, EarlyView.
Homologous recombination (HR) in Schizosaccharomyces pombe is essential when mating‐type switching–induced double‐strand breaks (DSBs) form at the mat1 locus. In h90 and h− strains, efficient mat1 DSB formation renders HR indispensable unless suppressors block these breaks.
Peter Kolesar, Siarhei Paliavoi, Barbora Stefanovie, Jan Josef Palecek   +3 more
wiley   +1 more source

Phosphorylation-dependent assembly and coordination of the DNA damage checkpoint apparatus by Rad4TopBP1 [PDF]

, 2013
The BRCT-domain protein Rad4(TopBP1) facilitates activation of the DNA damage checkpoint in Schizosaccharomyces pombe by physically coupling the Rad9-Rad1-Hus1 clamp, the Rad3(ATR) -Rad26(ATRIP) kinase complex, and the Crb2(53BP1) mediator.
Alcasabas, Antony M. Carr, Antony W. Oliver, Boos, Cescutti, Christopher P. Wardlaw, Du, Du, Du, Esashi, Fenech, Fukuura, Furuya, Garcia, Gong, Jing-Yi Ren, Kilkenny, Kumagai, Kumagai, Kumagai, Laurence H. Pearl, Lee, Lee, Li-Lin Du, Lin, Mathieu Rappas, Matthew Day, Meng Qu, Qu, Rappas, Rothbauer, Saka, Shiozaki, Smits, Stucki, Takeishi, Tanaka, Taylor, Valerie Garcia, Williams, Willson   +40 more
core   +1 more source

Genetic dissection reveals distinct contributions of the eS31 N‐terminal domain to translational accuracy in Saccharomyces cerevisiae

The FEBS Journal, EarlyView.
The eukaryote‐specific N‐terminal domain (NTD) of eS31 uses two distinct strategies to maintain translation fidelity. During elongation, a positively charged “hotspot” fine‐tunes the selection of incoming aa‐tRNA. During termination, the entire NTD acts as a structural scaffold to ensure the correct positioning of the release factor eRF1.
Qingxuan Gao, Hiroshi Otsuka, Kei Endo, Koichi Ito   +3 more
wiley   +1 more source

Extensive mass spectrometry-based analysis of the fission yeast proteome: the Schizosaccharomyces pombe PeptideAtlas [PDF]

, 2013
We report a high quality and system-wide proteome catalogue covering 71% (3,542 proteins) of the predicted genes of fission yeast, Schizosaccharomyces pombe, presenting the largest protein dataset to date for this important model organism.
Aebersold, Alexa, Anderson, Baerenfaller, Beck, Benjamini, Beyer, Bitton, Brockmann, Brunner, Chen, Craig, Davis, de Godoy, de Graaf, Deutsch, Deutsch, Elias, Geer, Ghaemmaghami, Greenbaum, Gygi, Humphrey, Huttlin, Ishihama, Ishihama, Keller, Kuster, Lackner, Lackner, Lam, Lam, Lam, Lam, Marguerat, Mata, Nagaraj, Nesvizhskii, Perkins, Reiter, Remacle, Sakharkar, Savas, Schmidt, Schmidt, Schrimpf, Sherwood, Shevchenko, Shteynberg, Sipiczki, Vizcaíno, Warringer, Wilhelm, Wood, Wood, Wood, Wu, Zhang   +57 more
core   +1 more source

Plants synthesize ergothioneine, showing a link to abiotic stress

Plant Biology, EarlyView.
Various plants possess ergothioneine biosynthetic genes and can synthesize this antioxidant, where increased EGT levels under abiotic stress conditions indicate protective functions. Abstract Ergothioneine (EGT) is a sulphur‐containing histidine derivative and a potent antioxidant that exhibits beneficial effects on human health.
C. Kock, N. Gutsche, S. Walter, S. Zachgo   +3 more
wiley   +1 more source