Results 231 to 240 of about 221,267 (266)

Expanding Genetic Code to Generate Human Brain Organoids with Both Vasculature and Microglia‐Like Cells

Advanced Science, EarlyView.
Using genetic code expansion, we engineered vascularized human cerebral organoids (vhCOs) with microglia‐like cells and blood‐brain barrier features. vhCOs recapitulate neurovascular interactions, regional identities, and neuronal subtypes resembling the fetal brain.
Haishuang Lin, Yaqing Wang, Hu Du, Yuxin Qin, Haoyu Zhang, Peng Wang, Lin Wei, Jianhua Qin   +7 more
wiley   +1 more source

A benchmark of semi-supervised scRNA-seq integration methods in real-world scenarios. [PDF]

PLoS Comput Biol
Shen X, He C, Guan L.
europepmc   +1 more source

Mapping Embryonic Mouse Lung Development Using Enhanced Spatial Transcriptomics

Advanced Science, EarlyView.
The mammalian lung contains ∼50 cell types arranged along the airway epithelium. Here, deterministic barcoding sequencing (DBiT‐seq) is optimized to nearly double transcript recovery, enabling highly sensitive spatial mapping of the embryonic mouse lung.
Pengfei Zhang, Benjamin K. Law, Niles Huang, Katharine Goodwin, Celeste M. Nelson, Michelle M. Chan   +5 more
wiley   +1 more source

Integrating scRNA-seq and snRNA-seq with spatial transcriptomics to unlock the xylem puzzle. [PDF]

Genome Biol
Wei M, Hsieh JA, Dang JF, Tong B, Li H, Sederoff R, Lin YJ, Li Q.   +7 more
europepmc   +1 more source

Upadacitinib Restrains the Pathogenic Fitness of CD4+ T Cells and Aberrant B Cell Programming in Optic Neuritis

Advanced Science, EarlyView.
Single‐cell profiling and functional perturbation reveal coordinated JAK1‐pSTAT3 downstream programs in optic neuritis, including MCL1‐dependent fitness of pathogenic CD4+ Tem cells and glycolysis‐linked, cholesterol‐sensitive B‐cell responses associated with RORA. Upadacitinib disrupts this reciprocal T‐B‐cell circuit and alleviates neuroinflammation,
Gengchen Jiang, Qi Jiang, Lian Han, Yue Peng, Xingyu Liu, Tong Yang, Hao Huang, Jiaying Liu, Yuan Gao, Hui Yang, Wenjun Zou, Zhaohuai Li, Wenru Su   +12 more
wiley   +1 more source

Immunohistochemical analysis and scRNA-Seq identifies vascular endothelial CXCR4 expression as a predictor of poor prognosis in pancreatic ductal adenocarcinoma. [PDF]

Transl Oncol
Imamura S, Uehara T, Iwaya M, Asaka S, Sugenoya S, Sawaguchi H, Kuraishi Y, Nakajima T, Komamura S, Nakamura A, Kimura T, Iwaya Y, Shimizu A, Soejima Y, Ota H, Nagaya T.   +15 more
europepmc   +1 more source

An Advanced Single-Cell RNA Sequencing (scRNA-seq) Protocol Utilizing Custom-Designed Multiplexing. [PDF]

Bio Protoc
Gao F, Liu X, Sun F, Xiao Y, Xiao G, Qu Z.   +5 more
europepmc   +1 more source

Shared and Divergent Transcriptional Programs of Oligodendrocyte Differentiation Across Vertebrate Species Revealed by scRNA-seq Analysis. [PDF]

Int J Mol Sci
Yun T, Park J, Baek M.
europepmc   +1 more source

CellTypeAI: Automated cell identification for scRNA-seq using local generative-AI

Daw RH, Deijnen HR, Rattray M, Grainger JR.   +3 more
europepmc   +1 more source