Results 91 to 100 of about 15,145,761 (252)

Delivery of DNA‐encoded vaccines and proteins in mice using cutaneous suction‐mediated transfection

FEBS Open Bio, EarlyView.
Cutaneous suction‐mediated transfection in mice for delivery of DNA‐encoded vaccines and proteins. A critical limitation of DNA vaccines and other therapeutics is transfection in vivo to produce the encoded antigens or therapeutic proteins. Cutaneous suction‐based methods have demonstrated effectiveness in many animal models and have been successfully ...
Emran O. Lallow, Isabel Brandtjen, Yaxin Mo, Melissa Gulley, Louis Osorio, Sagar Kudchodkar, Nandita C. Jhumur, Christine C. Roberts, Lisa K. Denzin, David I. Shreiber, Biju Parekkadan, Hao Lin, Joel N. Maslow   +12 more
wiley   +1 more source

AYOUS WOOD: MAIN USES AND SELECTED PHYSICAL AND MECHANICAL PROPERTIES OF AN INCREASINGLY INTERESTING MATERIAL ORIGINATING FROM CAMEROON

Drewno, 2022
Emiliano GENNARI, Rodolfo PICCHIO, Angela LO MONACO   +2 more
doaj   +1 more source

Variations in selected soil physical properties with landforms and slope within an inland valley ecosystem in Ashanti region of Ghana

Soil and Water Research, 2011
One peculiar feature of the inland valleys abundant in West Africa is their site-specific hydrology, underlain mainly by the prevailing landforms and topography.
Sunday E. Obalum, John C. Nwite, James Oppong, Charles A. Igwe, Toshiyuki Wakatsuki   +4 more
doaj   +1 more source

Purification and preparation of Marchantia polymorpha Auxin Response Factor 2 for phase separation studies

FEBS Open Bio, EarlyView.
We describe detailed protocols for the purification and preparation of Marchantia polymorpha Auxin Response Factor 2 (MpARF2). This protein is fused to an MBP solubility tag and an mNG fluorescent tag and is purified from Escherichia coli. The presented procedures make it possible to study MpARF2 assemblies, which could arise from phase separation ...
Bas Janssen, Robin Romein, Willy A. M. van den Berg, Simon Lindhoud, Jan Willem Borst, Carlo P. M. van Mierlo   +5 more
wiley   +1 more source

GelMA‐based 3D spheroids recapitulate transcriptomic and functional hallmarks of myeloid sarcoma

FEBS Open Bio, EarlyView.
GelMA 5% hydrogels support the formation of myeloid leukemia spheroids that recapitulate MS‐specific features, including G1 arrest, apoptosis, and ECM‐driven transcriptomic reprogramming. The 3D model mimicked soft‐tissue‐like stiffness and oxygen conditions, and transcriptomic convergence with primary MS samples confirmed its utility as a preclinical ...
Nicolas Germain, Elise Buret, Le Hoang Thanh Nguyen, Marion Cocheteux, Nicolas Touya, Mélanie Dhayer, Romain Dubois, Frank Lafont, Sebastien Janel, Patrice Maboudou, Steve Lancel, Philippe Marchetti   +11 more
wiley   +1 more source

The impact of fire frequency on selected soil physical properties in a semi-arid savannah Thornveld

Acta Agriculturae Scandinavica. Section B, Soil and Plant Science, 2019
The use of fire in rangeland management is standard practice, but the impact of fire frequency on soil physical properties is still not properly understood. In this study, the effect of fire frequency on selected physical properties was studied in a long-
M. I. Magomani, J. J. van Tol
doaj   +1 more source

Salmonella enterica serovar typhi limits the potency of typhoid toxin and ADP‐ribosylating toxin AB to establish a persistent infection

FEBS Open Bio, EarlyView.
The two catalytic subunits of typhoid toxin dissociate from the holotoxin in the ER of an intoxicated cell, but only CdtB exits the ER to generate immunosuppressive effects. PltA is retained in the ER and sequestered from its cytosolic target, thus allowing the anti‐inflammatory effects of CdtB to promote intestinal colonization.
Maria C. Zabala‐Rodriguez, Juliette K. Tinker, Teresa Frisan, Taj Azarian, Ken Teter   +4 more
wiley   +1 more source

Effects Of Stubble Burning on Selected Physical and Chemical Properties in Alkaline Agricultural Soil

ISPEC Journal of Agricultural Sciences
Stubble burning is a traditional soil management method that affects soil quality and fertility and has often been preferred by farmers. In this study conducted to understand the short-term effects of stubble burning on soil properties in alkaline ...
Mustafa DEMİR, Erdihan TUNÇ , Ömer ÇELİK , Nevzat ASLAN   +3 more
doaj   +1 more source

Using fluorescence fluctuation spectroscopy to quantify molecular dynamics within cells

FEBS Open Bio, EarlyView.
Microscopy techniques like fluorescence correlation spectroscopy (FCS) and number and brightness analysis (N&B) allow to quantify concentrations, diffusion rates, and oligomerization of fluorescently labeled molecules in the living cell. In this protocol the principles of how to set up these types of experiments are described including calibration ...
Mark A. Hink
wiley   +1 more source

Extruded Food Pellets with the Addition of Lucerne Sprouts: Selected Physical and Chemical Properties

Applied Sciences
The study aimed to develop extruded snack pellets enriched with fresh lucerne sprouts and to evaluate the effects of this type of additive and processing parameters on extrusion-cooking efficiency, specific energy consumption, and selected physical and ...
Beata Biernacka, Jakub Soja, Karolina Wojtunik-Kulesza, Marek Gancarz, Mateusz Stasiak, Magdalena Kręcisz, Maciej Combrzyński   +6 more
doaj   +1 more source