Rapid identification of plasmalogen molecular species using targeted multiplexed selected reaction monitoring mass spectrometry [PDF]
Journal of Mass Spectrometry and Advances in the Clinical Lab, 2021 Plasmalogens (Pls) levels are reported to be altered in several neurological and metabolic diseases. Identification of sn-1 fatty alcohols and sn-2 fatty acids of different Pls species is necessary to determine the roles and mechanisms of action of Pls ...
Abul Kalam Azad +7 more
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Bayesian Classification of Proteomics Biomarkers from Selected Reaction Monitoring Data using an Approximate Bayesian Computation-Markov Chain Monte Carlo Approach [PDF]
Cancer Informatics, 2018 Selected reaction monitoring (SRM) has become one of the main methods for low-mass-range–targeted proteomics by mass spectrometry (MS). However, in most SRM-MS biomarker validation studies, the sample size is very small, and in particular smaller than ...
Kashyap Nagaraja, Ulisses Braga-Neto
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Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins. [PDF]
PLoS ONE, 2020 Ribosomes are the sophisticated machinery that is responsible for protein synthesis in a cell. Recently, quantitative mass spectrometry (qMS) have been successfully applied for understanding the dynamics of protein complexes.
Yuishin Kosaka +6 more
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Absolute quantification of transcription factors in human erythropoiesis using selected reaction monitoring mass spectrometry [PDF]
STAR Protocols, 2020 Summary: Quantitative changes in transcription factor (TF) abundance regulate dynamic cellular processes, including cell fate decisions. Protein copy number provides information about the relative stoichiometry of TFs that can be used to determine how ...
Mark A. Gillespie +5 more
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Plasma preparation to measure FDA‐approved protein markers by selected reaction monitoring [PDF]
Clinical and Translational Medicine, 2015 BackgroundThe development of commercially available panels for human blood plasma screening via selected reaction monitoring (SRM) offers reliable, cost‐efficient and highly‐standardized discovery and validation of protein biomarkers.
Olga I. Kiseleva +6 more
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Selected Reaction Monitoring (SRM) Analysis of Epidermal Growth Factor Receptor (EGFR) in Formalin Fixed Tumor Tissue [PDF]
Clinical Proteomics, 2012 Background Analysis of key therapeutic targets such as epidermal growth factor receptor (EGFR) in clinical tissue samples is typically done by immunohistochemistry (IHC) and is only subjectively quantitative through a narrow dynamic range.
Hembrough Todd +13 more
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Variance component analysis to assess protein quantification in biomarker validation: application to selected reaction monitoring-mass spectrometry [PDF]
BMC Bioinformatics, 2018 Background In the field of biomarker validation with mass spectrometry, controlling the technical variability is a critical issue. In selected reaction monitoring (SRM) measurements, this issue provides the opportunity of using variance component ...
Amna Klich +12 more
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Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy [PDF]
Plant Methods, 2019 Background Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in glandular cells.
Ayako Takemori +6 more
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Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry. [PDF]
J Proteome Res, 2021 Cheung HYF +9 more
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Targeted selected reaction monitoring mass spectrometric immunoassay for insulin-like growth factor 1. [PDF]
PLoS ONE, 2013 Insulin-like growth factor 1 (IGF1) is an important biomarker of human growth disorders that is routinely analyzed in clinical laboratories. Mass spectrometry-based workflows offer a viable alternative to standard IGF1 immunoassays, which utilize various
Eric E Niederkofler +10 more
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