Results 1 to 10 of about 289,825 (222)

Development of Mass Spectrometry Selected Reaction Monitoring Method for Quantitation and Pharmacokinetic Study of Stepharine in Rabbit Plasma [PDF]

Advances in Pharmacological Sciences, 2014
Highly sensitive liquid chromatography mass spectrometry method on triple quadrupole (QQQ) mass spectrometer was successfully applied for pharmacokinetic study of stepharine in rabbit plasma.
Arthur T. Kopylov, Ksenia G. Kuznetsova, Olga M. Mikhailova, Andrey G. Moshkin, Vladimir V. Turkin, Andrei A. Alimov   +5 more
doaj   +4 more sources

Alterations in Whey Protein Abundance Correlated with the Somatic Cell Count Identified via Label-Free and Selected Reaction Monitoring Proteomic Approaches [PDF]

Animals
The somatic cell count (SCC) is widely used to assess milk quality and diagnose intramammary infections. Several whey proteins have been shown to correlate significantly with SCC and are considered potential indicators of udder health.
Jing Li, Kaixu Chen, Changjiang Zang, Xiaowei Zhao, Zhiqiang Cheng, Xiaobin Li, Caidie Wang, Yong Chen, Kailun Yang   +8 more
doaj   +3 more sources

Rapid identification of plasmalogen molecular species using targeted multiplexed selected reaction monitoring mass spectrometry [PDF]

Journal of Mass Spectrometry and Advances in the Clinical Lab, 2021
Plasmalogens (Pls) levels are reported to be altered in several neurological and metabolic diseases. Identification of sn-1 fatty alcohols and sn-2 fatty acids of different Pls species is necessary to determine the roles and mechanisms of action of Pls ...
Abul Kalam Azad, Hironori Kobayashi, Abdullah Md. Sheikh, Harumi Osago, Hiromichi Sakai, Md. Ahsanul Haque, Shozo Yano, Atsushi Nagai   +7 more
doaj   +2 more sources

Selected reaction monitoring for the quantification of Escherichia coli ribosomal proteins. [PDF]

PLoS ONE, 2020
Ribosomes are the sophisticated machinery that is responsible for protein synthesis in a cell. Recently, quantitative mass spectrometry (qMS) have been successfully applied for understanding the dynamics of protein complexes.
Yuishin Kosaka, Wataru Aoki, Megumi Mori, Shunsuke Aburaya, Yuta Ohtani, Hiroyoshi Minakuchi, Mitsuyoshi Ueda   +6 more
doaj   +2 more sources

Absolute quantification of transcription factors in human erythropoiesis using selected reaction monitoring mass spectrometry [PDF]

STAR Protocols, 2020
Summary: Quantitative changes in transcription factor (TF) abundance regulate dynamic cellular processes, including cell fate decisions. Protein copy number provides information about the relative stoichiometry of TFs that can be used to determine how ...
Mark A. Gillespie, Carmen G. Palii, Daniel Sanchez-Taltavull, Theodore J. Perkins, Marjorie Brand, Jeffrey A. Ranish   +5 more
doaj   +2 more sources

Plasma preparation to measure FDA‐approved protein markers by selected reaction monitoring [PDF]

Clinical and Translational Medicine, 2015
BackgroundThe development of commercially available panels for human blood plasma screening via selected reaction monitoring (SRM) offers reliable, cost‐efficient and highly‐standardized discovery and validation of protein biomarkers.
Olga I. Kiseleva, Yulia A. Romashova, Natalia E. Moskaleva, Natalia A. Petushkova, Nadezhda B. Teryaeva, Artem Yu. Belyaev, Andrey V. Lisitsa   +6 more
doaj   +2 more sources

Variance component analysis to assess protein quantification in biomarker validation: application to selected reaction monitoring-mass spectrometry [PDF]

BMC Bioinformatics, 2018
Background In the field of biomarker validation with mass spectrometry, controlling the technical variability is a critical issue. In selected reaction monitoring (SRM) measurements, this issue provides the opportunity of using variance component ...
Amna Klich, Catherine Mercier, Laurent Gerfault, Pierre Grangeat, Corinne Beaulieu, Elodie Degout-Charmette, Tanguy Fortin, Pierre Mahé, Jean-François Giovannelli, Jean-Philippe Charrier, Audrey Giremus, Delphine Maucort-Boulch, Pascal Roy   +12 more
doaj   +2 more sources

Quantitative assay of targeted proteome in tomato trichome glandular cells using a large-scale selected reaction monitoring strategy [PDF]

Plant Methods, 2019
Background Glandular trichomes found in vascular plants are called natural cell factories because they synthesize and store secondary metabolites in glandular cells.
Ayako Takemori, Taiken Nakashima, Hisashi Ômura, Yuki Tanaka, Keisuke Nakata, Hiroshi Nonami, Nobuaki Takemori   +6 more
doaj   +2 more sources

Targeted selected reaction monitoring mass spectrometric immunoassay for insulin-like growth factor 1. [PDF]

PLoS ONE, 2013
Insulin-like growth factor 1 (IGF1) is an important biomarker of human growth disorders that is routinely analyzed in clinical laboratories. Mass spectrometry-based workflows offer a viable alternative to standard IGF1 immunoassays, which utilize various
Eric E Niederkofler, David A Phillips, Bryan Krastins, Vathany Kulasingam, Urban A Kiernan, Kemmons A Tubbs, Scott M Peterman, Amol Prakash, Eleftherios P Diamandis, Mary F Lopez, Dobrin Nedelkov   +10 more
doaj   +2 more sources

Targeted Phosphoinositides Analysis Using High-Performance Ion Chromatography-Coupled Selected Reaction Monitoring Mass Spectrometry. [PDF]

J Proteome Res, 2021
Cheung HYF, Coman C, Westhoff P, Manke M, Sickmann A, Borst O, Gawaz M, Watson SP, Heemskerk JWM, Ahrends R.   +9 more
europepmc   +3 more sources