Results 31 to 40 of about 289,825 (222)
Integrated LC-MS/MS system for plant metabolomics
Computational and Structural Biotechnology Journal, 2013 Liquid chromatography-tandem mass spectrometry (LC-MS/MS) is highly sensitive, selective, and enables extensive detection of metabolites within a sample.
Yuji Sawada, Masami Yokota Hirai
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MRMaid: The SRM Assay Design Tool for Arabidopsis and Other Species
Frontiers in Plant Science, 2012 Selected reaction monitoring (SRM), sometimes called multiple reaction monitoring (MRM), is becoming the tool of choice for targeted quantitative proteomics in the plant science community. Key to a successful SRM experiment is prior identification of the
Jun eFan +3 more
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Frontiers in Bioengineering and Biotechnology, 2020 Targeted proteomics is a mass spectrometry-based protein quantification technique with high sensitivity, accuracy, and reproducibility. As a key component in the multi-omics toolbox of systems biology, targeted liquid chromatography-selected reaction ...
Yuqian Gao +40 more
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Genes and Environment, 2018 Background Nucleoside triphosphates participate in fundamental cellular processes as building blocks of DNA and RNA, energy carriers, and cofactors in enzymatic reactions, and their balance is tightly regulated.
Shun Matsuda, Toshihiko Kasahara
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Biomedicines, 2020 Selected reaction monitoring (SRM) is a mass spectrometric technique characterized by the exceptionally high selectivity and sensitivity of protein detection.
Sergey Radko +10 more
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Monitoring casbene synthase in Jatropha curcas tissues using targeted proteomics
Plant Methods, 2021 Background Casbene synthase (CS) is responsible for the first committed step in the biosynthesis of phorbol esters (PE) in the Euphorbiaceae. PE are abundant in the seeds of the biofuel crop Jatropha curcas and its toxicity precludes the use of the ...
Natália Pinto de Almeida +6 more
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Selected reaction monitoring for quantitative proteomics: a tutorial
Molecular Systems Biology, 2008 Systems biology relies on data sets in which the same group of proteins is consistently identified and precisely quantified across multiple samples, a requirement that is only partially achieved by current proteomics approaches.
Vinzenz Lange +3 more
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Frontiers in Plant Science, 2013 One major constraint upon the application of molecular crop breeding approaches is the small number of genes linked to agronomically desirable traits through defined biochemical mechanisms.
Richard P. Jacoby +2 more
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Regulation of yeast central metabolism by enzyme phosphorylation
Molecular Systems Biology, 2012 As a frequent post‐translational modification, protein phosphorylation regulates many cellular processes. Although several hundred phosphorylation sites have been mapped to metabolic enzymes in Saccharomyces cerevisiae, functionality was demonstrated for
Ana Paula Oliveira +5 more
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Targeted proteomics reveals compositional dynamics of 60S pre‐ribosomes after nuclear export
Molecular Systems Biology, 2012 Construction and intracellular targeting of eukaryotic pre‐ribosomal particles involve a multitude of diverse transiently associating trans‐acting assembly factors, energy‐consuming enzymes, and transport factors.
Martin Altvater +7 more
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