Results 211 to 220 of about 22,058 (247)
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SELEX: Just another separation?
The Analyst, 2005The potential for quickly isolating high affinity, highly selective ligands generated much excitement when SELEX was first described. Fifteen years later, SELEX has still not achieved widespread acceptance due to limitations in aptamer affinity, stability and throughput.
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Aptamers: The “evolution” of SELEX
Methods, 2016It has been more than two decades since the first aptamer molecule was discovered. Since then, aptamer molecules have gain much attention in the scientific field. This increasing traction can be attributed to their many desirable traits, such as 1) their potentials to bind a wide range of molecules, 2) their malleability, and 3) their low cost of ...
Yi Xi, Wu, Young Jik, Kwon
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2014
Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
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Systematic evolution of ligands by exponential enrichment (SELEX) protocol is a valuable technique to identify RNA aptamers interacting with RNA structural motifs. RNA aptamers are mainly resolved with affinity column chromatography and electrophoretic mobility shift assay (EMSA).
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Analytical and Bioanalytical Chemistry, 2006
SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification.
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SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification.
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A Computational Study of Alternate SELEX
Bulletin of Mathematical Biology, 2014Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure for identifying nucleic acid (NA) molecules with affinities for specific target species, such as proteins, peptides, or small organic molecules. Here, we extend the work in Seo et al. (Bull Math Biol 72:1623-1665, 2010) (multiple-target SELEX or positive SELEX) and examine
Seo, Yeon-Jung +2 more
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2009 International Symposium on Micro-NanoMechatronics and Human Science, 2009
The pattern of genome transcription in prokaryotes is determined by selective distribution of RNA polymerase within the genome. The gene selectivity of RNA polymerase is modulated after interaction with DNA-binding transcription factors (TFs). Escherichia coli contains a total of about 300 TFs, but the regulatory function has not yet been identified ...
Tomohiro Shimada +3 more
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The pattern of genome transcription in prokaryotes is determined by selective distribution of RNA polymerase within the genome. The gene selectivity of RNA polymerase is modulated after interaction with DNA-binding transcription factors (TFs). Escherichia coli contains a total of about 300 TFs, but the regulatory function has not yet been identified ...
Tomohiro Shimada +3 more
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Proceedings of the 14th Conference on Pattern Languages of Programs, 2007
State machine specifications and their implementations are often complex because they have many responsibilities mixed together. A potential cause for responsibility clutter is message multiplexing, which means that one or more incoming and/or outgoing messages of the state machine contain data that belongs to different concerns.
Frank Roessler, Birgit Geppert
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State machine specifications and their implementations are often complex because they have many responsibilities mixed together. A potential cause for responsibility clutter is message multiplexing, which means that one or more incoming and/or outgoing messages of the state machine contain data that belongs to different concerns.
Frank Roessler, Birgit Geppert
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SELEX experiment (Fermilab E781)
AIP Conference Proceedings, 1998The poster presents the physics goals and experimental status of Selex (Segmented Large X Spectrometer), supported by many international institutions which has been taking data since February 1997. The principal goal of this experiment is to have a high statistics sample of charm baryons from 650 GeV Σ− and π− beams.
Fernanda G. Garcia, Jurgen Simon
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Developing Aptamers by Cell-Based SELEX
2016The reliable targeting of cell surface disease-associated proteins is a major challenge in chemical biology and molecular medicine. In this regard, aptamers represent a very attractive and innovative class of ligand molecules. Aptamers are generated by a reiterated in vitro procedure, named SELEX (Systematic Evolution of Ligands by Exponential ...
Catuogno S, Esposito CL, de Franciscis V
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Combinatorial Chemistry of Nucleic Acids: SELEX
Molecular Biology, 2000A method of irrational oligonucleotide design, SELEX, is considered. Individual SELEX products, aptamers, are small molecules (40–100 nt) that have a unique three-dimensional structure, which provides for their specific and high-affinity binding to targets varying from low-molecular-weight ligands to proteins.
A M, Kopylov, V A, Spiridonova
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