The Iranian Journal of Veterinary Science and Technology, 2021 Aptamers are oligonucleotides that can be easily synthesized and bind to their targets with high affinity and specificity. Several aptamers specific to soluble factors of coagulation cascade have been produced, however, aptamers specific to platelet cell membrane molecules have not been reported yet.
Fatemeh Alemi +4 more
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SELEX-based DNA Aptamer Selection: A Perspective from the Advancement of Separation Techniques
Analytical Sciences, 2020 DNA aptamers, which are short, single-stranded DNA sequences that selectively bind to target substances (proteins, cells, small molecules, metal ions), can be acquired by means of the systematic evolution of ligands by exponential enrichment (SELEX) methodology.
Shingo Saito
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Oligonucleotide Functionalised Microbeads: Indispensable Tools for High-Throughput Aptamer Selection [PDF]
Molecules, 2015 The functionalisation of microbeads with oligonucleotides has become an indispensable technique for high-throughput aptamer selection in SELEX protocols.
Lewis A. Fraser +7 more
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Computational Design of a New Aflatoxin B1 Aptamerin lieuof SELEX Technique [PDF]
, 2022 AbstractMycotoxins are extremely dangerous, and their detection in our environment, food and feed is becoming increasingly important. Biosensors are being implemented heavily in mycotoxin detection along with other significant applications. Aptamers have numerous beneficial advantages as biorecognition molecules and are being used as the biorecognition
Mohamad Yasser Ahmad Ghazy
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RSC Advances, 2020 Highly specific and selective aptamers for Lucentis were selected using 10 rounds of a modified and highly stringent SELEX process.
Tanu Bhardwaj +2 more
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Rapid one-step selection method for generating nucleic acid aptamers: development of a DNA aptamer against α-bungarotoxin. [PDF]
PLoS ONE, 2012 BACKGROUND: Nucleic acids based therapeutic approaches have gained significant interest in recent years towards the development of therapeutics against many diseases.
Lasse H Lauridsen +4 more
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Development of a QCM-D-Based Aptasensor for the Real-Time Detection of β-Lactoglobulin [PDF]
BiosensorsThe prevalence of food allergies has been steadily increasing in recent years. β-lactoglobulin (β-LG), the main allergenic protein of milk and dairy allergies, is more commonly observed in infants and children.
Tuba Çanak-Ipek +5 more
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Selection of binding targets in parasites using phage-display and aptamer libraries in vivo and in vitro [PDF]
Frontiers in Immunology, 2013 Parasite infections are largely dependent on interactions between pathogen and different host cell populations to guarantee a successful infectious process.
Renata Rosito Tonelli +2 more
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Advantages, applications, and future directions of in vivo aptamer SELEX: A review [PDF]
Molecular Therapy: Nucleic AcidsIn vivo systematic evolution of ligands by exponential enrichment (SELEX) emerged as a transformative technique for directly identifying aptamers within living organisms.
Minchuan Lyu +4 more
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Bioinformatical Prediction of G-quadruplex Aptamer for Detection of a Ligand in Practice [PDF]
Journal of Applied Sciences and Nanotechnology, 2023 Considering the introduction of aptamers as a new generation of analyte identifiers, this class of materials can be used in diagnostic systems because aptamers are easier to produce, more sensitive, higher accuracy, less sensitive to environmental ...
Mohammad Reza Hosseini VeleshKolaei +3 more
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