Results 1 to 10 of about 20,710 (205)

Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]

PLoS ONE, 2010
BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann, Tanja Gesell, Doris Chen, Christina Lorenz, Renée Schroeder   +4 more
doaj   +6 more sources

Inside the Black Box: What Makes SELEX Better?

Molecules, 2019
Aptamers are small oligonucleotides that are capable of binding specifically to a target, with impressive potential for analysis, diagnostics, and therapeutics applications.
Natalia Komarova, Alexander E Kuznetsov
exaly   +3 more sources

Recent Advances in the Optimization of Nucleic Acid Aptamers and Aptasensors [PDF]

Biosensors
Nucleic acid aptamers are single-stranded DNA or RNA molecules that can bind to a target with high specificity and affinity, as screened by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX).
Yuan Wang, Mengyan Nie
doaj   +2 more sources

The Potential Use of Aptamers in The Process of Drug Development [PDF]

Pharmaceutical Sciences, 2021
Single-stranded nucleic acids can fold and create unique 3-dimensional structures when interacting with other molecules. The unique structure can achieve high specificity and affinity for the particular target.
Tooba Gholikhani, Balam Jimenez Brito, Frey Livingston, Shalen Kumar   +3 more
doaj   +1 more source

Development of DNA aptamer selection approach based on membrane ultrafiltration of aptamer/target complex

Acta Biomedica Scientifica, 2022
Background. Aptamers are small single-stranded DNA or RNA molecules that have an affinity for a specific target molecule. The main method of aptamers construction is the technology of systematic evolution of ligands with exponential enrichment (SELEX ...
I. S. Solovarov, M. A. Khasnatinov, N. A. Liapunova, I. G. Kondratov, G. A. Danchinova   +4 more
doaj   +1 more source

Engineering Novel Aptameric Fluorescent Biosensors for Analysis of the Neurotoxic Environmental Contaminant Insecticide Diazinon from Real Vegetable and Fruit Samples

Frontiers in Bioscience-Landmark, 2022
Background: Diazinon is a widely used organophosphorus neurotoxic insecticide. It is a common environmental contaminant and a hazardous agri-waste. Its detection is critical to control entry into food systems and protect the environment. Methods: In this
Mai-Huong Thi Can, Ulhas Sopanrao Kadam, Kien Hong Trinh, Yuhan Cho, Hyebi Lee, Yujeong Kim, Sundong Kim, Chang Ho Kang, Sang Hee Kim, Woo Sik Chung, Sang Yeol Lee, Jong Chan Hong   +11 more
doaj   +1 more source

Surveillance Unattended Foliage Penetrating Radar for Border Control and Homeland Protection [PDF]

TransNav, 2013
The increasing request for safety, security and environment protection at local and national level reveal the deficiency of the traditional surveillance and control centers to satisfy the needs and requirements of modern border control systems for ...
Felicia Amato, Alfonso Farina, Michele Fiorini, Sergio Gallone   +3 more
doaj   +1 more source

Complementary chain competition and fluorescence quenching detection of Deoxynivalenol and analytical applications using a novel aptamer

CyTA - Journal of Food, 2021
Deoxynivalenol (DON) is a mycotoxin commonly found in cereals and animal feeds, which also is a carcinogen easily soluble in water. So it has drawn considerable interest in food safety.
Mingwei Qin, Xiaomeng Zhang, Xinyue Zhao, Yuzhu Song, Jinyang Zhang, Xueshan Xia, Qinqin Han   +6 more
doaj   +1 more source

Imaging the coupling of terahertz radiation to a high electron mobility transistor in the near-field [PDF]

Journal of the European Optical Society-Rapid Publications, 2009
We used AlGaN/GaN high electron mobility transistors as room-temperature direct detectors of radiation at 0.15 THz from a free electron laser, hence 5 times higher than their cutoff frequency of 30 GHz.
Ortolani M., Di Gaspare A., Giovine E., Evangelisti F., Foglietti V., Doria A., Gallerano G. P., Giovenale E., Messina G., Spassovsky I., Lanzieri C., Peroni M., Cetronio A.   +12 more
doaj   +1 more source

Aptamers and antibodies: rivals or allies in cancer targeted therapy?

Exploration of Targeted Anti-tumor Therapy, 2021
The goal of an efficacious cancer therapy is to specifically target diseased cells at high accuracy while sparing normal, healthy cells. Over the past three decades, immunotherapy, based on the use of monoclonal antibodies (mAbs) directed against tumor ...
Lisa Agnello, Simona Camorani, Monica Fedele, Laura Cerchia   +3 more
doaj   +1 more source