Results 41 to 50 of about 20,710 (205)

Quantitative modeling and data analysis of SELEX experiments

, 2005
SELEX (Systematic Evolution of Ligands by Exponential Enrichment) is an experimental procedure that allows extracting, from an initially random pool of DNA, those oligomers with high affinity for a given DNA-binding protein. We address what is a suitable
Djordjevic, Marko, Sengupta, Anirvan M.
core   +1 more source

Combined aptamer and transcriptome sequencing of single cells. [PDF]

, 2018
The transcriptome and proteome encode distinct information that is important for characterizing heterogeneous biological systems. We demonstrate a method to simultaneously characterize the transcriptomes and proteomes of single cells at high throughput ...
Abate, Adam R, Delley, Cyrille L, Liu, Leqian, Sarhan, Maen F   +3 more
core   +2 more sources

Enhancing Small Molecule Sensing With Aptameric Functionalized Nano Devices

Advanced Materials Technologies, EarlyView.
Unveiling an ultra‐sensitive, non‐invasive neurotransmitter sensor. For the first time, a nanoscale sensor for detecting an important neurotransmitter was demonstrated using micro‐electromechanical systems (MEMS) technology. Our approach utilized field‐effect transistor (FET)‐based readout to enable pico‐molar detection of biomarkers in sweat.
Thi Thanh Ha Nguyen, Cong Minh Nguyen, Quang Thang Trinh, Minh Anh Huynh, Khanh Vinh Ho, Dang Co Pham, Dzung Dao, Sven Ingebrandt, Sina Jamali, Xuan Thang Vu, Nam‐Trung Nguyen, Tuan‐Khoa Nguyen   +11 more
wiley   +1 more source

Comparison of Whole-Cell SELEX Methods for the Identification of Staphylococcus Aureus-Specific DNA Aptamers

Sensors, 2015
Whole-cell Systemic Evolution of Ligands by Exponential enrichment (SELEX) is the process by which aptamers specific to target cells are developed. Aptamers selected by whole-cell SELEX have high affinity and specificity for bacterial surface molecules ...
Jihea Moon, Giyoung Kim, Saet Byeol Park, Jongguk Lim, Changyeun Mo   +4 more
doaj   +1 more source

Measurement of the Ds lifetime [PDF]

, 2001
We report precise measurement of the Ds meson lifetime. The data were taken by the SELEX experiment (E781) spectrometer using 600 GeV/c Sigma-, pi- and p beams. The measurement has been done using 918 reconstructed Ds.
A Kushnirenko, A Lamberto, A Morelos, A Ocherashvili, A Penzo, A.A Vorobyov, A.G Atamantchouk, A.G Dolgolenko, A.G Krivshich, A.I Kulyavtsev, A.I Sitnikov, A.M.F Endler, A.N Vasiliev, A.P Kozhevnikov, A.P Nilov, A.V Evdokimov, A.V Nemitkin, Aitala, B.V Razmyslovich, Bigi, Bonvicini, C Mao, C Newsom, C.O Escobar, D Mao, D Skow, D.V Vavilov, E Gülmez, E McCliment, E Ozel, E Ramberg, E.M Leikin, Engelfried, F.G Garcia, Frabetti, Frixione, G Alkhazov, G.B Dzyubenko, G.F Rappazzo, G.P Thomas, G.V Davidenko, Groom, H Krüger, He Kangling, I Eschrich, I Giller, I Konorov, I Larin, I.S Filimonov, J Engelfried, J Kilmer, J Lach, J Russ, J Simon, J You, K Vorwalter, K.D Nelson, L Emediato, L Stutte, L.G Landsberg, L.J Dauwe, L.M Kochenda, L.N Uvarov, M Gaspero, M Iori, M Kaya, M Luksys, M Mattson, M Procario, M Srivastava, M Svoiski, M.A Kubantsev, M.A Moinester, M.Y Balatz, N.F Bondar, N.K Terentyev, N.P Kuropatkin, P Gouffon, P Mathew, P Pogodin, P Schiavon, P.S Cooper, P.V Neoustroev, R Edelstein, R Zukanovich-Funchal, Russ, S Kwan, S Ozkorucuklu, S Zheng, S.B Nurushev, S.V Petrenko, S.Y Jun, T Lungov, U Dersch, V Matveev, V Steiner, V Stepanov, V.A Prutskoi, V.A Victorov, V.E Vishnyakov, V.F Kurshetsov, V.I Rud, V.J Smith, V.L Golovtsov, V.P Kubarovsky, V.P Maleev, V.S Verebryusov, V.T Kim, V.V Molchanov, W Zhaog, Y Li, Y Onel, Z Mao   +112 more
core   +2 more sources

Hijacking the Host Clock: A Nematode Effector Antagonizes Soybean Circadian Defense and Translation Control

Advanced Science, EarlyView.
Soybean employs its circadian clock, governed by GmCCA1, to rhythmically defend against soybean cyst nematodes. The pathogen retaliates by secreting the effector Hg4E02, which hijacks the clock to suppress defense and co‐opt the host's translation machinery for nutrient acquisition.
Xingwei Wang, Yufeng Xu, Yanfei Hu, Lijun Cao, Ru Jiang, Changtian Chen, Rick Masonbrink, Thomas Maier, Yuchen Tu, Yabo Shi, Enhui Liu, Lingan Kong, Chan Guo, Wei Zhao, Peng Shi, Wenzhen Du, Andrew Severin, Thomas Baum, Deliang Peng, Huan Peng, Mian Zhou, Wei Wang   +21 more
wiley   +1 more source

A mathematical analysis of SELEX [PDF]

Computational Biology and Chemistry, 2007
Systematic evolution of ligands by exponential enrichment (SELEX) is a procedure by which a mixture of nucleic acids that vary in sequence can be separated into pure components with the goal of isolating those with specific biochemical activities. The basic idea is to combine the mixture with a specific target molecule and then separate the target-NA ...
Howard A, Levine, Marit, Nilsen-Hamilton
openaire   +2 more sources

Aptamer-Conjugated Gold Nanoparticles Targeting Epidermal Growth Factor Receptor Variant III for the Treatment of Glioblastoma

International Journal of Nanomedicine, 2020
Li Peng,1,2,* Yanling Liang,1,* Xinxin Zhong,1 Zhiman Liang,1,2 Yinghong Tian,3 Shuji Li,1 Jingxue Liang,4 Ransheng Wang,4 Yuqi Zhong,4 Yusheng Shi,5 Xingmei Zhang1 1Key Laboratory of Mental Health of the Ministry of Education, Guangdong-Hong Kong-Macao ...
Peng L, Liang Y, Zhong X, Liang Z, Tian Y, Li S, Liang J, Wang R, Zhong Y, Shi Y, Zhang X   +10 more
doaj  

Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

Molecules, 2017
Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice.
Kamal Rahimizadeh, Hadi AlShamaileh, Milena Fratini, Madhuri Chakravarthy, Michelle Stephen, Sarah Shigdar, Rakesh N. Veedu   +6 more
doaj   +1 more source

High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro

STAR Protocols, 2022
Summary: High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro.
Raphaël Pantier, Kashyap Chhatbar, Grace Alston, Heng Yang Lee, Adrian Bird   +4 more
doaj   +1 more source