Results 51 to 60 of about 12,731 (219)

Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures

Molecules, 2017
Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice.
Kamal Rahimizadeh, Hadi AlShamaileh, Milena Fratini, Madhuri Chakravarthy, Michelle Stephen, Sarah Shigdar, Rakesh N. Veedu   +6 more
doaj   +1 more source

The SELEX phototube RICH detector [PDF]

Nuclear Instruments and Methods in Physics Research Section A: Accelerators, Spectrometers, Detectors and Associated Equipment, 1999
25 pages with 14 figures included.
Engelfried, J., Filimonov, I., Kilmer, J., Kozhevnikov, A., Kubarovsky, V., Molchanov, V., Nemitkin, A., Ramberg, E., Rud, V., Stutte, L.   +9 more
openaire   +2 more sources

Aptamer‐Directed Porous DNA Nanocomposite Hydrogel for Active Pulp Preservation: Immunomodulation, Stem Cell Recruitment and Reparative Dentinogenesis

Advanced Science, EarlyView.
This study presents an injectable DNA‐based porous hydrogel integrating catechol motifs and targeting aptamers for pulpitis management. Upon in situ crosslinking, the scaffold actively recruits endogenous dental pulp stem cells, restores redox homeostasis, and modulates immune responses.
Luhui Cai, Huan Yu, Zihan Ni, Kan Chen, I‐Chen Tsai, Zeyu Bao, Shuo Sun, Weichang Li, Yunhua Chen, Qiong Xu   +9 more
wiley   +1 more source

The SELEX and Neutral SELEX experiments.

, 2013
The normal SELEX experiment (shown with black arrows) begins with transcription of a DNA pool into RNA using T7 RNA polymerase. The RNAs then undergo counter selection against sequences binding non-specifically to the filter, followed by binding to the ...
Doris Chen (367236), Christina Lorenz (13577), Renée Schroeder (9110), Bob Zimmermann (367235), Tanja Gesell (51149)   +4 more
core   +1 more source

Aptamer‐Targeted PrPC Drives Colorectal Cancer Metastasis via a LYN‐STAT3 Complex and Enables Liquid Biopsy Detection

Advanced Science, EarlyView.
The aptamer WHY‐3E identifies PrPC as a CRC driver. Stabilized by USP18, endocytosed PrPC forms a LYN/STAT3 complex, upregulating MSN transcription to promote metastasis. Crucially, WHY‐3E sensitively detects PrPC‐positive circulating exosomes, establishing a robust theoretical foundation for non‐invasive clinical diagnostics.
Chunlin Wang, Hongyu Wu, Chaojing Zheng, Hao Zhang, Xiaoqiu Wu, Jiaqi Wang, Zewen Chang, Jun Xiang, Yunxiao Liu, Chenkai Zhang, Yuliuming Wang, Hao Jiang, Yuchen Zhong, Jun Luo, Ying Chen, NaNa Zhang, Weiyuan Zhang, Ziming Yuan, ChaoXia Zou, Weihong Tan, Meng Wang, Hanqing Hu, Tao Bing, Guiyu Wang   +23 more
wiley   +1 more source

A novel hybrid approach to overcome defects of CE-SELEX and cell-SELEX in developing aptamers against aspartate β-hydroxylase

Research in Pharmaceutical Sciences
Background and purpose: Aptamers, a new category of molecular probes, are overthrowing antibodies in molecular diagnostics. However, there are serious problems with using aptamers for this application including poor or non-specific binding in vivo ...
Hadi Bakhtiari, Hamed Naghoosi, Sina Sattari, Mahmoud Vahidi, Mehdi Shakouri Khomartash, Ali Faridfar, Mohsen Rajaeinejad, Mohsen Nikandish   +7 more
doaj   +1 more source

Multi-Output Power Converter, Operated from a Regulated Input Bus, for the Sireus Rate Sensor

E3S Web of Conferences, 2017
This paper describes a DC to DC converter designed to meet the power supply requirements of the SiREUS Coarse Rate Sensor (CRS) which is a 3-axis MEMS Rate Sensor (MRS) that uses a resonating ring gyro and will be used in different ESA missions.
Torrecilla Marcos Compadre, Weinberg Alan H, Hutton Patrick, Clark Craig   +3 more
doaj   +1 more source

Nucleic Acid Therapeutics for “Undruggable” Cancer Targets: Mechanisms, Challenges, and Prospects

Advanced Science, EarlyView.
Nucleic acid therapeutics bypass the structural limitations of conventional drugs by targeting mRNA rather than proteins. This review examines how antisense oligonucleotides, siRNAs, miRNAs, aptamers, and mRNA vaccines intervene against historically undruggable oncoproteins including Ras, MYC, and p53, highlighting mechanistic advances, delivery ...
Feng Xu, Ke Wang, Kaizhong Lu, Tianlun Hou, Yuan Chen, Xian Wang, Hongchuan Jin   +6 more
wiley   +1 more source

Aptamer Cell-Based Selection: Overview and Advances

Biomedicines, 2017
Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools.
Silvia Catuogno, Carla Lucia Esposito
doaj   +1 more source

Non-SELEX Selection of Aptamers

, 2016
Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification.
Michael Musheev (1469377), Andrei Drabovich (2058694), Maxim Berezovski (2488750), Sergey N. Krylov (314119)   +3 more
core   +1 more source