Results 51 to 60 of about 19,390 (205)
Use of cell-SELEX to generate DNA aptamers as molecular probes of HPV-associated cervical cancer cells. [PDF]
PLoS ONE, 2012 Disease-specific biomarkers are an important tool for the timely and effective management of pathological conditions, including determination of susceptibility, diagnosis, and monitoring efficacy of preventive or therapeutic strategies.
Jessica C Graham, Helmut Zarbl
doaj +1 more source
Development of Cell-Specific Aptamers: Recent Advances and Insight into the Selection Procedures
Molecules, 2017 Systematic evolution of ligands by exponential enrichment (SELEX) is an established procedure for developing short single-stranded nucleic acid ligands called aptamers against a target of choice.
Kamal Rahimizadeh +6 more
doaj +1 more source
International Journal of Molecular Sciences, 2022 Brucellae are Gram-negative, aerobic, non-motile coccobacilli causing brucellosis in man and animals. The disease is one of the most significant yet neglected global zoonoses.
Dalia M. El-Husseini +5 more
semanticscholar +1 more source
Research in Pharmaceutical SciencesBackground and purpose: Aptamers, a new category of molecular probes, are overthrowing antibodies in molecular diagnostics. However, there are serious problems with using aptamers for this application including poor or non-specific binding in vivo ...
Hadi Bakhtiari +7 more
doaj +1 more source
Non-SELEX Selection of Aptamers
, 2016 Aptamers are typically selected from libraries of random DNA (or RNA) sequences by SELEX, which involves multiple rounds of alternating steps of partitioning and PCR amplification.
Michael Musheev (1469377) +3 more
core +1 more source
Aptamer Cell-Based Selection: Overview and Advances
Biomedicines, 2017 Aptamers are high affinity single-stranded DNA/RNA molecules, produced by a combinatorial procedure named SELEX (Systematic Evolution of Ligands by Exponential enrichment), that are emerging as promising diagnostic and therapeutic tools.
Silvia Catuogno, Carla Lucia Esposito
doaj +1 more source
Cornea‐SELEX for aptamers targeting the surface of eyes and liposomal drug delivery
ExplorationCornea is the major barrier to drug delivery to the eye, which results in low bioavailability and poor efficacy of topical eye treatment. In this work, we first select cornea‐binding aptamers using tissue‐SELEX on pig cornea.
Ka-Ying Wong +3 more
semanticscholar +1 more source
Implantable electrochemical sensors for continuous blood monitoring
FlexMat, EarlyView.Implantable electrochemical sensors enable direct, in situ, and continuous blood monitoring, shifting diagnosis from population‐based averages to dynamic, individualized analysis. This review systematically examines the influence of the unique blood properties on sensor design.
Kuangyi Zou +3 more
wiley +1 more source
Schematic of Adherent Cell-SELEX (AC-SELEX) Procedure.
, 2013 A starting library of >1015 aptamers was utilized and eighteen rounds of AC-SELEX were carried out to purify and amplify the aptamers which recognized epitopes present on HF cells and not present on HeLa cells.
Jessica C. Graham (325676) +1 more
core +1 more source
Journal of Biological Engineering, 2020 Aptamers, single-stranded DNAs or RNAs, can be selected from a library containing random sequences using a method called Systematic Evolution of Ligands by EXponential Enrichment (SELEX).
Qing-Xiu Liu +6 more
semanticscholar +1 more source