Results 11 to 20 of about 19,390 (205)

Capture-SELEX: Selection Strategy, Aptamer Identification, and Biosensing Application

Biosensors, 2022
Small-molecule contaminants, such as antibiotics, pesticides, and plasticizers, have emerged as one of the substances most detrimental to human health and the environment.
Sin Yu Lam, Hill Lam Lau, Chun Kit Kwok
doaj   +3 more sources

Monitoring genomic sequences during SELEX using high-throughput sequencing: neutral SELEX. [PDF]

PLoS ONE, 2010
BACKGROUND: SELEX is a well established in vitro selection tool to analyze the structure of ligand-binding nucleic acid sequences called aptamers. Genomic SELEX transforms SELEX into a tool to discover novel, genomically encoded RNA or DNA sequences ...
Bob Zimmermann, Tanja Gesell, Doris Chen, Christina Lorenz, Renée Schroeder   +4 more
doaj   +2 more sources

RAPID-SELEX for RNA aptamers. [PDF]

PLoS ONE, 2013
Aptamers are high-affinity ligands selected from DNA or RNA libraries via SELEX, a repetitive in vitro process of sequential selection and amplification steps.
Kylan Szeto, David R Latulippe, Abdullah Ozer, John M Pagano, Brian S White, David Shalloway, John T Lis, Harold G Craighead   +7 more
doaj   +3 more sources

Quantitative Comparison of Capture-SELEX, GO-SELEX, and Gold-SELEX for Enrichment of Aptamers.

Analytical Chemistry, 2023
Since 1990, numerous methods for aptamer selection have been developed, although a quantitative comparison of their sequence enrichment is lacking. In this study, we compared the enrichment factors of three library-immobilization SELEX methods (capture ...
Yuzhe Ding, Jue-Wen Liu
semanticscholar   +3 more sources

SELEX against Whole-Cell Bacteria Resulted in Lipopolysaccharide Binding Aptamers.

Journal of Biotechnology, 2022
Nucleic acid aptamers are target-specific oligonucleotides selected from combinatorial libraries through an iterative in vitro screening process known as Systemic Evolution of Ligands by Exponential Enrichment (SELEX).
D. Yılmaz, Tugdem Muslu, A. Parlar, Hasan Kurt, Meral Yüce   +4 more
semanticscholar   +2 more sources

Advances in Aptamer-Based Biosensors and Cell-Internalizing SELEX Technology for Diagnostic and Therapeutic Application

Biosensors, 2022
Aptamers are a group of synthetic single-stranded nucleic acids. They are generated from a random library of single-stranded DNA or RNA by a technology named systematic evolution of ligands by exponential enrichment (SELEX). SELEX is a repetitive process
Zixuen Gan, Muhamad Aidilfitri Mohamad Roslan, Mohd Yunus Abd Shukor, Murni Halim, Nur Adeela Yasid, Jaafar Abdullah, Ina Salwany Md Yasin, Helmi Wasoh   +7 more
doaj   +2 more sources

Aptamer Screening: Current Methods and Future Trend towards Non-SELEX Approach

Biosensors
Aptamers are nucleic acid sequences that specifically bind with target molecules and are vital to applications such as biosensing, drug development, disease diagnostics, etc.
Zhihui Fang, Xiaorui Feng, Fan Tang, Han Jiang, Shuyuan Han, Ran Tao, Chenze Lu   +6 more
doaj   +2 more sources

IMPATIENT-qPCR: monitoring SELEX success during in vitro aptamer evolution

Applied Microbiology and Biotechnology
SELEX (Systematic Evolution of Ligands by Exponential enrichment) processes aim on the evolution of high-affinity aptamers as binding entities in diagnostics and biosensing.
Ann-Kathrin Kissmann, G. Bolotnikov, Runliu Li, Franziska Müller, Hu Xing, Markus Krämer, K. Gottschalk, Jakob Andersson, Tanja Weil, F. Rosenau   +9 more
semanticscholar   +2 more sources

High-throughput sequencing SELEX for the determination of DNA-binding protein specificities in vitro

STAR Protocols, 2022
Summary: High-throughput sequencing SELEX (HT-SELEX) is a powerful technique for unbiased determination of preferred target motifs of DNA-binding proteins in vitro.
Raphaël Pantier, Kashyap Chhatbar, Grace Alston, Heng Yang Lee, Adrian Bird   +4 more
doaj   +2 more sources

Capture-SELEX: Selection of DNA Aptamers for Aminoglycoside Antibiotics [PDF]

Journal of Analytical Methods in Chemistry, 2012
Small organic molecules are challenging targets for an aptamer selection using the SELEX technology (SELEX—Systematic Evolution of Ligans by EXponential enrichment).
Regina Stoltenburg, Nadia Nikolaus, Beate Strehlitz   +2 more
doaj   +2 more sources